cDNA Clone KCC1

• Synonyms: SLC12A4
• Plasmid: pCMV5 FLAG KCC1 T983E
• Parent Plasmid: pCMV5D FLAG
• Expressed: FLAG-KCC1 T983E
• Genbank: NM_005072
• Tag: FLAG
• Species: Human

Sequence of Insert

ATGCCTCACTTCACCGTGGTGCCAGTGGACGGGCCGAGGCGCGGCGACTA
TGACAACCTCGAGGGGCTCAGTTGGGTGGACTACGGGGAGCGCGCCGAGC
TGGATGACTCGGACGGACATGGCAACCACAGAGAGAGCAGCCCTTTTCTT
TCCCCCTTGGAGGCTTCCAGAGGAATTGACTACTATGACAGGAACCTGGC
ACTGTTTGAGGAAGAGCTGGACATCCGCCCAAAGGTATCGTCTCTTCTGG
GAAAGCTCGTCAGCTACACCAACCTCACCCAGGGCGCCAAAGAGCATGAG
GAGGCCGAGAGTGGGGAGGGCACCCGCCGGAGGGCAGCCGAGGCACCCAG
CATGGGCACCCTCATGGGGGTGTACCTGCCCTGCCTGCAGAATATCTTTG
GGGTTATCCTCTTCCTGCGGCTGACCTGGATGGTGGGCACAGCAGGTGTG
CTACAGGCCCTCCTCATCGTGCTTATCTGCTGCTGTTGTACCCTGCTGAC
GGCCATCTCCATGAGTGCCATCGCCACCAACGGTGTGGTTCCAGCTGGGG
GCTCCTATTTCATGATCTCTCGTTCACTGGGGCCAGAATTTGGAGGTGCT
GTGGGCCTGTGCTTCTACCTGGGAACAACATTCGCAGCAGCCATGTACAT
CCTGGGGGCCATCGAGATCTTGCTGACCTACATTGCCCCACCAGCTGCCA
TTTTTTACCCATCGGGTGCTCATGACACGTCGAATGCCACTTTGAACAAT
ATGCGTGTGTATGGGACCATTTTCCTGACCTTCATGACCCTGGTGGTGTT
TGTGGGGGTCAAGTATGTGAACAAATTTGCCTCGCTCTTCCTGGCCTGTG
TGATCATCTCCATCCTCTCCATCTATGCTGGGGGCATAAAGTCTATATTT
GACCCTCCCGTGTTTCCGGTATGCATGCTGGGCAACAGGACCCTGTCCCG
GGACCAGTTTGACATCTGTGCCAAGACAGCTGTAGTGGACAATGAGACAG
TGGCCACCCAGCTATGGAGTTTCTTCTGCCACAGCCCCAACCTTACGACC
GACTCCTGTGACCCCTACTTCATGCTCAACAATGTGACCGAGATCCCTGG
CATCCCCGGGGCAGCTGCTGGTGTGCTCCAGGAAAACCTGTGGAGCGCCT
ACCTGGAGAAGGGTGACATCGTGGAGAAGCATGGGCTGCCCTCCGCAGAT
GCCCCGAGCCTGAAGGAGAGCCTGCCTCTGTACGTGGTCGCTGACATCGC
CACATCCTTCACCGTGCTGGTCGGCATCTTCTTCCCTTCTGTAACAGGCA
TCATGGCTGGCTCAAACCGCTCTGGGGACCTTCGTGACGCCCAGAAGTCT
ATCCCTGTGGGGACCATTCTGGCCATCATTACAACTTCCCTCGTGTACTT
CAGCAGTGTGGTTCTCTTTGGTGCCTGCATTGAGGGTGTGGTTCTCCGGG
ACAAGTATGGCGATGGTGTCAGCAGGAACTTGGTGGTGGGCACACTGGCC
TGGCCTTCACCCTGGGTCATCGTCATCGGCTCCTTCTTTTCAACGTGTGG
CGCTGGCCTCCAGAGCCTCACAGGGGCACCACGCCTATTGCAGGCCATTG
CCAAGGACAACATCATCCCCTTCCTCCGGGTGTTTGGCCACGGGAAGGTG
AATGGTGAACCCACATGGGCACTCCTCCTGACGGCACTCATCGCCGAGCT
GGGCATCCTCATCGCCTCCCTCGACATGGTGGCCCCCATCTTATCCATGT
TCTTTCTGATGTGCTACCTGTTCGTGAACCTCGCCTGTGCGGTGCAGACA
CTCCTGAGGACCCCCAACTGGCGGCCCCGGTTCAAGTACTATCACTGGGC
GCTGTCCTTCCTGGGCATGAGTCTCTGCCTGGCCCTTATGTTTGTCTCCT
CCTGGTACTATGCCCTGGTGGCCATGCTCATCGCCGGCATGATCTACAAA
TACATCGAGTACCAAGGGGCTGAGAAGGAGTGGGGTGACGGGATCCGAGG
CCTGTCCCTGAGCGCTGCCCGCTACGCGCTGTTGCGGCTGGAGGAGGGGC
CTCCTCACACCAAGAACTGGCGGCCGCAGCTGCTGGTGCTGCTGAAGCTG
GACGAGGACCTCCACGTGAAGTACCCGCGGCTCCTCACCTTCGCCTCCCA
GCTCAAGGCTGGCAAGGGCCTGACCATTGTTGGTTCTGTCATCCAGGGGA
GCTTCTTGGAGAGCTATGGCGAGGCTCAGGCCGCCGAGCAGACCATCAAG
AACATGATGGAAATTGAGAAGGTGAAGGGCTTCTGCCAGGTGGTGGTGGC
CAGCAAGGTGCGGGAGGGGCTGGCCCACCTCATCCAGTCCTGTGGCCTGG
GAGGCATGCGGCATAACTCCGTGGTGCTGGGCTGGCCCTACGGCTGGCGA
CAGAGCGAGGACCCCCGTGCCTGGAAGACCTTCATTGACACCGTGCGCTG
CACTACGGCTGCCCACCTGGCCCTGCTCGTGCCCAAGAACATCGCCTTCT
ACCCCAGCAACCACGAGCGCTACCTGGAGGGCCACATAGACGTGTGGTGG
ATCGTGCACGATGGTGGCATGCTCATGCTTCTGCCCTTCCTGCTGCGCCA
GCATAAGGTCTGGAGGAAGTGCCGGATGCGCATCTTCACAGTGGCCCAGA
TGGATGACAACAGCATCCAGATGAAGAAGGACCTGGCTGTCTTTCTGTAC
CATCTGCGCCTTGAGGCCGAGGTGGAGGTGGTGGAGATGCATAACAGTGA
CATCTCTGCATACACCTACGAGCGGACGCTGATGATGGAGCAGCGGTCGC
AGATGCTGCGGCAGATGAGACTGACCAAGACTGAGCGGGAGCGAGAAGCC
CAGCTGGTCAAGGATCGGCACTCGGCCCTGAGGCTGGAGAGCCTGTACTC
GGACGAGGAAGATGAGTCTGCAGTGGGGGCTGACAAGATCCAGATGGAGT
GGACCAGGGACAAGTACATGACTGAGACCTGGGACCCCAGCCATGCCCCT
GACAATTTCCGGGAGCTGGTGCACATTAAGCCGGACCAATCCAATGTGCG
GCGCATGCACACTGCTGTGAAGCTCAATGAAGTCATTGTCACGCGCTCCC
ACGACGCCCGCCTGGTTCTCCTAAACATGCCTGGCCCACCCAGGAACAGT
GAGGGCGACGAGAACTACATGGAGTTCCTCGAGGTGCTGACCGAGGGCCT
TGAGCGGGTGCTGTTGGTGCGCGGTGGTGGCCGTGAAGTCATCACCATCT
ACTCCTGAgtcgac

Amino Acid Sequence

MDYKDDDDKGSFEEFAMPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAEL
DDSDGHGNHRESSPFLSPLEASRGIDYYDRNLALFEEELDIRPKVSSLLG
KLVSYTNLTQGAKEHEEAESGEGTRRRAAEAPSMGTLMGVYLPCLQNIFG
VILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGG
SYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAI
FYPSGAHDTSNATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACV
IISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTAVVDNETV
ATQLWSFFCHSPNLTTDSCDPYFMLNNVTEIPGIPGAAAGVLQENLWSAY
LEKGDIVEKHGLPSADAPSLKESLPLYVVADIATSFTVLVGIFFPSVTGI
MAGSNRSGDLRDAQKSIPVGTILAIITTSLVYFSSVVLFGACIEGVVLRD
KYGDGVSRNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIA
KDNIIPFLRVFGHGKVNGEPTWALLLTALIAELGILIASLDMVAPILSMF
FLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSS
WYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGP
PHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGS
FLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHLIQSCGLG
GMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFY
PSNHERYLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQM
DDNSIQMKKDLAVFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQ
MLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGADKIQMEW
TRDKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSH
DARLVLLNMPGPPRNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIY
S*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44351
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: T983E