cDNA Clone CISD1

• Synonyms: mitoNEET, ZCD1, C10orf70, MDS029
• Plasmid: pET15b SUMO TEVb CISD1
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6xHIS-SUMO-TEVb-CISD1
• Genbank: NM_018464.4
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCCGAAAACCTGTATTTTCAGATGAGTCTGACTTCCAGTTCCAGCGTACGAGTTGAATGGATCGCAGCAGTTACCATTGCTGCTGGGACAGCTGCAATTGGTTATCTAGCTTACAAAAGATTTTATGTTAAAGATCATCGAAATAAAGCTATGATAAACCTTCACATCCAGAAAGACAACCCCAAGATAGTACATGCTTTTGACATGGAGGATTTGGGAGATAAAGCTGTGTACTGCCGTTGTTGGAGGTCCAAAAAGTTCCCATTCTGTGATGGGGCTCACACAAAACATAACGAAGAGACTGGAGACAATGTGGGCCCTCTGATCATCAAGAAAAAAGAAACTTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSENLYFQMSLTSSSSVRVEWIAAVTIAAGTAAIGYLAYKRFYVKDH
RNKAMINLHIQKDNPKIVHAFDMEDLGDKAVYCRCWRSKKFPFCDGAHTK
HNEETGDNVGPLIIKKKET*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44400
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cleavage with TEV releases protein at M1 start.