cDNA Clone MO25 beta

• Synonyms: CAB39L, MO2L
• Plasmid: pGEX6P-1 MYC MO25b K300M
• Parent Plasmid: pGEX6P1
• Expressed: GST-MYC-MO25b K300M
• Genbank: NM_001079670.1
• Tag: GST
• Tag: Myc
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGAGCAGAAGCTGATCTCTGAAGAGGACTTGCCTTTGTTTAGTAAATCACACAAAAATCCAGCAGAAATTGTGAAAATCCTGAAAGACAATTTGGCCATTTTGGAAAAGCAAGACAAAAAGACAGACAAGGCTTCAGAAGAAGTGTCTAAATCACTGCAAGCAATGAAAGAAATTCTGTGTGGTACAAACGAGAAAGAACCCCCGACAGAAGCAGTGGCTCAGCTAGCACAAGAACTCTACAGCAGTGGCCTGCTGGTGACACTGATAGCTGACCTGCAGCTGATAGACTTTGAGGGAAAAAAAGATGTGACCCAGATATTTAACAACATCTTGAGAAGACAGATAGGCACTCGGAGTCCTACTGTGGAGTATATTAGTGCTCATCCTCATATCCTGTTTATGCTCCTCAAAGGATATGAAGCCCCACAGATTGCCTTACGTTGTGGGATTATGCTGAGAGAATGTATTCGACATGAACCACTTGCCAAAATCATCCTCTTTTCTAATCAATTCAGAGATTTCTTTAAGTACGTGGAGTTGTCAACATTTGATATTGCTTCAGATGCCTTTGCTACTTTCAAGGATTTACTAACCAGACATAAAGTGTTGGTAGCAGACTTCTTAGAACAAAATTACGACACTATTTTTGAAGACTATGAGAAATTGCTTCAGTCTGAGAATTATGTTACTAAGAGACAGTCTTTAAAGCTGCTAGGGGAGCTGATCCTGGACCGTCACAACTTTGCCATCATGACAAAGTATATCAGCAAGCCGGAGAACCTGAAACTCATGATGAACCTCCTTCGGGATAAAAGTCCCAACATCCAGTTTGAAGCCTTTCATGTTTTTAAGGTGTTTGTGGCCAGTCCTCACAAAACACAGCCTATTGTGGAGATCCTGTTAAAAAATCAGCCCATGCTCATTGAGTTTCTGAGCAGCTTCCAAAAAGAAAGGACGGATGATGAGCAGTTCGCTGACGAGAAGAACTACTTGATTAAACAGATCCGAGACTTGAAGAAAACGGCCCCTTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMEQKLISEEDLPLFSKS
HKNPAEIVKILKDNLAILEKQDKKTDKASEEVSKSLQAMKEILCGTNEKE
PPTEAVAQLAQELYSSGLLVTLIADLQLIDFEGKKDVTQIFNNILRRQIG
TRSPTVEYISAHPHILFMLLKGYEAPQIALRCGIMLRECIRHEPLAKIIL
FSNQFRDFFKYVELSTFDIASDAFATFKDLLTRHKVLVADFLEQNYDTIF
EDYEKLLQSENYVTKRQSLKLLGELILDRHNFAIMTKYISKPENLKLMMN
LLRDKSPNIQFEAFHVFKVFVASPHKTQPIVEILLKNQPMLIEFLSSFQK
ERTDDEQFADEKNYLIKQIRDLKKTAP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44410
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Gene product differs at n-terminus as compared to reference, starting MLPLFSK rather than MKKMPLFSK. Contains K300M mutation.