cDNA Clone IsoT (USP5)

• Synonyms: USP5
• Plasmid: pGEX6P2 MCS-Linker1-IsoT UBP domain (V173-T289)
• Parent Plasmid: pGEX6P2
• Expressed: GST-MCS-Linker1-IsoT UBP domain (V173-T289)
• Genbank: NM_001098536.1 GI:148727330
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGGCTAGTGAAAACTTCGCGAGTCGCGCCGTAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAACAACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGAGCTGACGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGCATCGACATGCTGAAGATGCAGAAGACATAAGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAASENFASR
AVVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCG
RRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDP
SLAEHLSHFGIDMLKMQKT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45359
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mutagenesis to kill 3' Not1 so Not1 in MCS is unique