cDNA Clone TRAF6

• Synonyms: MGC:3310; RNF85
• Plasmid: pGEX6P TRAF6 M1-D285 (has S12F* from DU1758)
• Parent Plasmid: pGEX6P1
• Expressed: GST-TRAF6 M1-D285
• Genbank: NM_145803.2 GI:332000008 with change c35t (S12F*) and a456g (silent)
• Tag: GST
• Species: Human

Sequence of Insert

AGATCTATGAGTCTGCTAAACTGTGAAAACAGCTGTGGATTCAGCCAGTCTGAAAGTGACTGCTGTGTGGCCATGGCCAGCTCCTGTAGCGCTGTAACAAAAGATGATAGTGTGGGTGGAACTGCCAGCACGGGGAACCTCTCCAGCTCATTTATGGAGGAGATCCAGGGATATGATGTAGAGTTTGACCCACCCCTGGAAAGCAAGTATGAATGCCCCATCTGCTTGATGGCATTACGAGAAGCAGTGCAAACGCCATGCGGCCATAGGTTCTGCAAAGCCTGCATCATAAAATCAATAAGGGATGCAGGTCACAAATGTCCAGTTGACAATGAAATACTGCTGGAAAATCAACTATTTCCAGACAATTTTGCAAAACGTGAGATTCTTTCTCTGATGGTGAAATGTCCAAATGAAGGTTGTTTGCACAAGATGGAACTGAGACATCTTGAGGATCATCAGGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCAATGCCAGCGTCCCTTCCAAAAATTCCATATTAATATTCACATTCTGAAGGATTGTCCAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCATTTGAAGATAAAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCATCTGTGAATACTGCAATACTATACTCATCAGAGAACAGATGCCTAATCATTATGATCTAGACTGCCCTACAGCCCCAATTCCATGCACATTCAGTACTTTTGGTTGCCATGAAAAGATGCAGAGGAATCACTTGGCACGCCACCTACAAGAGAACACCCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAGTTTGAGCGTTATACCCGACTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLLNCENSCGFSQSESDC
CVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYE
CPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFP
DNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPF
QKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEYCN
TILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSH
MRMLAQAVHSLSVIPD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45369
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * This mutation derives from DU1758 used as template (Also present in other TRAF6 clones). Matches DQ891209.2 GI:123980611