cDNA Clone Ubiquitin

• Synonyms: -
• Plasmid: pET3a-Ubiquitin dimer -6HIS (wild type)
• Parent Plasmid: pET3a
• Expressed: Ubiquitin-Ubiquitin-6His
• Genbank: P62988
• Tag: 6His
• Species: Human

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCAGAGGCGGCATGCAGATCTTCGTGAAGA
CCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATT
GAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCA
GCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGT
CTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTC
AGAGGTGGGCATCATCACCATCACCATTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR
GGHHHHHH**

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET3a-Ubiquitin dimer -6HIS (wild type)62.92 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU23863
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: C-ter 6HIS added onto 23442
• Low Temp: No