cDNA Clone MIRO1

• Synonyms: ARHT1, MIRO-1, RHOT1
• Plasmid: pcDNA5frtTO eCFP Miro1 K572R
• Parent Plasmid: pcDNA5D FRT/TO FLAG CFP
• Expressed: eCFP-Miro1 K567R
• Genbank: NM_018307.3
• Tag: CFP
• Species: Human

Sequence of Insert

GGATCCATGAAGAAAGACGTGCGAATTCTGCTGGTGGGAGAACCTAGAGT
TGGGAAGACATCACTGATTATGTCTCTGGTCAGTGAAGAATTTCCAGAAG
AGGTTCCTCCCCGGGCAGAAGAAATCACCATTCCAGCTGATGTCACCCCA
GAGAGAGTTCCAACACACATTGTAGATTACTCAGAAGCAGAACAGAGTGA
TGAACAACTTCATCAAGAAATATCTCAGGCTAATGTCATCTGTATAGTGT
ATGCCGTTAACAACAAGCATTCTATTGATAAGGTAACAAGTCGATGGATT
CCTCTCATAAATGAAAGAACAGACAAAGACAGCAGGCTGCCTTTAATATT
GGTTGGGAACAAATCTGATCTGGTGGAATATAGTAGTATGGAGACCATCC
TTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGAGTGTTCA
GCGAAAAACCTGAAGAACATATCAGAGCTCTTTTATTACGCACAGAAAGC
TGTTCTTCATCCTACAGGGCCCCTGTACTGCCCAGAGGAGAAGGAGATGA
AACCAGCTTGTATAAAAGCCCTTACTCGTATATTTAAAATATCTGATCAA
GATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTCAGAGGAT
TTGTTTCAACACTCCATTAGCTCCTCAAGCTCTGGAGGATGTCAAGAATG
TAGTCAGAAAACATATAAGTGATGGTGTGGCTGACAGTGGGTTGACCCTG
AAAGGTTTTCTCTTTTTACACACACTTTTTATCCAGAGAGGGAGACACGA
AACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGACCTGGATT
TGACACCTGAATATTTGTTCCCCCTGCTGAAAATACCTCCTGATTGCACT
ACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGCACCTTTGACAA
GCATGATTTGGATAGAGACTGTGCTTTGTCACCTGATGAGCTTAAAGATT
TATTTAAAGTTTTCCCTTACATACCTTGGGGGCCAGATGTGAATAACACA
GTTTGTACCAATGAAAGAGGCTGGATAACCTACCAGGGATTCCTTTCCCA
GTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAATATTTGG
GCTATCTAGGCTATTCAATATTGACTGAGCAAGAGTCTCAAGCTTCAGCT
GTTACAGTGACAAGAGATAAAAAGATAGACCTGCAGAAAAAACAAACTCA
AAGAAATGTGTTCAGATGTAATGTAATTGGAGTGAAAAACTGTGGGAAAA
GTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCAGAAGAAA
ATTCGTGAAGATCATAAATCCTACTATGCGATTAACACTGTTTATGTATA
TGGACAAGAGAAATACTTGTTGTTGCATGATATCTCAGAATCGGAATTTC
TAACTGAAGCTGAAATCATTTGTGATGTTGTATGCCTGGTATATGATGTC
AGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACACTT
TATGGACAGCAGAATACCTTGCTTAATCGTAGCTGCAAAGTCAGACCTGC
ATGAAGTTAAACAAGAATACAGTATTTCACCTACTGATTTCTGCAGGAAA
CACAAAATGCCTCCACCACAAGCCTTCACTTGCAATACTGCTGATGCCCC
CAGTAAGGATATCTTTGTTAGATTGACAACAATGGCCATGTATCCGCACG
TGACACAAGCTGACCTCAAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTT
GGTGCTACTGTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAGCATTATT
GAAACAGCGATGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSMKKDVRILL
VGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYS
EAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDS
RLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELF
YYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAEL
NFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFI
QRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFL
QSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITY
QGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDL
QKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAI
NTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCAR
IFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTC
NTADAPSKDIFVRLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVLGFA
MYKALLKQR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44429
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Variant 3, K572R.