cDNA Clone DNMT1

• Plasmid: pSc-B-6HIS-DNMT1
• Parent Plasmid: pSC-B
• Genbank: NM_001130823.1
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCAATGCATCATCACCATCACCATATGCCGGCGCGTACCGCCCC
AGCCCGGGTGCCCACACTGGCCGTCCCGGCCATCTCGCTGCCCGACGATG
TCCGCAGGCGGCTCAAAGATTTGGAAAGAGACAGCTTAACAGAAAAGGAA
TGTGTGAAGGAGAAATTGAATCTCTTGCACGAATTTCTGCAAACAGAAAT
AAAGAATCAGTTATGTGACTTGGAAACCAAATTACGTAAAGAAGAATTAT
CCGAGGAGGGCTACCTGGCTAAAGTCAAATCCCTTTTAAATAAAGATTTG
TCCTTGGAGAACGGTGCTCATGCTTACAACCGGGAAGTGAATGGACGTCT
AGAAAACGGGAACCAAGCAAGAAGTGAAGCCCGTAGAGTGGGAATGGCAG
ATGCCAACAGCCCCCCCAAACCCCTTTCCAAACCTCGCACGCCCAGGAGG
AGCAAGTCCGATGGAGAGGCTAAGCGTTCAAGAGACCCTCCTGCCTCAGC
CTCCCAAGTAACTGGGATTAGAGCTGAACCTTCACCTAGCCCCAGGATTA
CAAGGAAAAGCACCAGGCAAACCACCATCACATCTCATTTTGCAAAGGGC
CCTGCCAAACGGAAACCTCAGGAAGAGTCTGAAAGAGCCAAATCGGATGA
GTCCATCAAGGAAGAAGACAAAGACCAGGATGAGAAGAGACGTAGAGTTA
CATCCAGAGAACGAGTTGCTAGACCGCTTCCTGCAGAAGAACCTGAAAGA
GCAAAATCAGGAACGCGCACTGAAAAGGAAGAAGAAAGAGATGAAAAAGA
AGAAAAGAGACTCCGAAGTCAAACCAAAGAACCAACACCCAAACAGAAAC
TGAAGGAGGAGCCGGACAGAGAAGCCAGGGCAGGCGTGCAGGCTGACGAG
GACGAAGATGGAGACGAGAAAGATGAGAAGAAGCACAGAAGTCAACCCAA
AGATCTAGCTGCCAAACGGAGGCCCGAAGAAAAAGAACCTGAAAAAGTAA
ATCCACAGATTTCTGATGAAAAAGACGAGGATGAAAAGGAGGAGAAGAGA
CGCAAAACGACCCCCAAAGAACCAACGGAGAAAAAAATGGCTCGCGCCAA
AACAGTCATGAACTCCAAGACCCACCCTCCCAAGTGCATTCAGTGCGGGC
AGTACCTGGACGACCCTGACCTCAAATATGGGCAGCACCCACCAGACGCG
GTGGATGAGCCACAGATGCTGACAAATGAGAAGCTGTCCATCTTTGATGC
CAACGAGTCTGGCTTTGAGAGTTATGAGGCGCTTCCCCAGCACAAACTGA
CCTGCTTCAGTGTGTACTGTAAGCACGGTCACCTGTGTCCCATCGACACC
GGCCTCATCGAGAAGAATATCGAACTCTTCTTTTCTGGTTCAGCAAAACC
AATCTATGATGATGACCCgTCTCTTGAAGGTGGTGTTAATGGCAAAAATC
TTGGCCCCATAAATGAATGGTGGATCACTGGCTTTGATGGAGGTGAAAAG
GCCCTCATCGGCTTCAGCACCTCATTTGCCGAATACATTCTGATGGATCC
CAGTCCCGAGTATGCGCCCATATTTGGGCTGATGCAGGAGAAGATCTACA
TCAGCAAGATTGTGGTGGAGTTCCTGCAGAGCAATTCCGACTCGACCTAT
GAGGACCTGATCAACAAGATCGAGACCACGGTTCCTCCTTCTGGCCTCAA
CTTGAACCGCTTCACAGAGGACTCCCTCCTGCGACACGCGCAGTTTGTGG
TGGAGCAGGTGGAGAGTTATGACGAGGCCGGGGACAGTGATGAGCAGCCC
ATCTTCCTGACgCCCTGCATGCGGGACCTGATCAAGCTGGCTGGGGTCAC
GCTGGGACAGAGGCGAGCCCAGGCGAGGCGGCAGACCATCAGGCATTCTA
CCAGGGAGAAGGACAGGGGACCCACGAAAGCCACCACCACCAAGCTGGTC
TACCAGATCTTCGATACTTTCTTCGCAGAGCAAATTGAAAAGGATGACAG
AGAAGACAAGGAGAACGCCTTTAAGCGCCGGCGATGTGGCGTCTGTGAGG
TGTGTCAGCAGCCTGAGTGTGGGAAATGTAAAGCCTGCAAGGACATGGTT
AAATTTGGTGGCAGTGGACGGAGCAAGCAGGCTTGCCAAGAGCGGAGGTG
TCCCAATATGGCCATGAAGGAGGCAGATGACGATGAGGAAGTCGATGATA
ACATCCCAGAGATGCCGTCACCCAAAAAAATGCACCAGGGGAAGAAGAAG
AAACAGAACAAGAATCGCATCTCTTGGGTCGGAGAAGCCGTCAAGACTGA
TGGGAAGAAGAGTTACTATAAGAAGGTGTGCATTGATGCGGAAACCCTGG
AAGTGGGGGACTGTGTCTCTGTTATTCCAGATGATTCCTCAAAACCGCTG
TATCTAGCAAGGGTCACGGCGCTGTGGGAGGACAGCAGCAACGGGCAGAT
GTTTCACGCCCACTGGTTCTGCGCTGGGACAGACACAGTCCTCGGGGCCA
CGTCGGACCCTCTGGAGCTGTTCTTGGTGGATGAATGTGAGGACATGCAG
CTTTCATATATCCACAGCAAAGTGAAAGTCATCTACAAAGCCCCCTCCGA
AAACTGGGCCATGGAGGGAGGCATGGATCCCGAGTCCCTGCTGGAGGGGG
ACGACGGGAAGACCTACTTCTACCAGCTGTGGTATGATCAAGACTACGCG
AGATTCGAGTCCCCTCCAAAAACCCAGCCAACAGAGGACAACAAGTTCAA
ATTCTGTGTGAGCTGTGCCCGTCTGGCTGAGATGAGGCAAAAAGAAATCC
CCAGGGTCCTGGAGCAGCTCGAGGACCTGGATAGCCGGGTCCTCTACTAC
TCAGCCACCAAGAACGGCATCCTGTACCGAGTTGGTGATGGTGTGTACCT
GCCCCCTGAGGCCTTCACGTTCAACATCAAGCTGTCCAGTCCCGTGAAAC
GCCCACGGAAGGAGCCCGTGGATGAGGACCTGTACCCAGAGCACTACCGG
AAATACTCCGACTACATCAAAGGCAGCAACCTGGATGCCCCTGAGCCCTA
CCGAATTGGCCGGATCAAAGAGATCTTCTGTCCCAAGAAGAGCAACGGCA
GGCCCAATGAGACTGACATCAAAATCCGGGTCAACAAGTTCTACAGGCCT
GAGAACACCCACAAGTCCACTCCAGCGAGCTACCACGCAGACATCAACCT
GCTCTACTGGAGCGACGAGGAGGCCGTGGTGGACTTCAAGGCTGTGCAGG
GCCGCTGCACCGTGGAGTATGGGGAGGACCTGCCCGAGTGCGTCCAGGTG
TACTCCATGGGCGGCCCCAACCGCTTCTACTTCCTCGAGGCCTATAATGC
AAAGAGCAAAAGCTTTGAAGATCCTCCCAACCATGCCCGTAGCCCTGGAA
ACAAAGGGAAGGGCAAGGGAAAAGGGAAGGGCAAGCCCAAGTCCCAAGCC
TGTGAGCCGAGCGAGCCAGAGATAGAGATCAAGCTGCCCAAGCTGCGGAC
CCTGGATGTGTTTTCTGGCTGCGGGGGGTTGTCGGAGGGATTCCACCAAG
CAGGCATCTCTGACACGCTGTGGGCCATCGAGATGTGGGACCCTGCGGCC
CAGGCGTTCCGGCTGAACAACCCCGGCTCCACAGTGTTCACAGAGGACTG
CAACATCCTGCTGAAGCTGGTCATGGCTGGGGAGACCACCAACTCCCGCG
GCCAGCGGCTGCCCCAGAAGGGAGACGTGGAGATGCTGTGCGGCGGGCCG
CCCTGCCAGGGCTTCAGCGGCATGAACCGCTTCAATTCGCGCACCTACTC
CAAGTTCAAAAACTCTCTGGTGGTTTCCTTCCTCAGCTACTGCGACTACT
ACCGGCCCCGGTTCTTCCTCCTGGAGAATGTCAGGAACTTTGTCTCCTTC
AAGCGCTCCATGGTCCTGAAGCTCACCCTCCGCTGCCTGGTCCGCATGGG
CTATCAGTGCACCTTCGGCGTGCTGCAGGCCGGTCAGTACGGCGTGGCCC
AGACTAGGAGGCGGGCCATCATCCTGGCCGCGGCCCCTGGAGAGAAGCTC
CCTCTGTTCCCGGAGCCACTGCACGTGTTTGCTCCCCGGGCCTGCCAGCT
GAGCGTGGTGGTGGATGACAAGAAGTTTGTGAGCAACATAACCAGGTTGA
GCTCGGGTCCTTTCCGGACCATCACGGTGCGAGACACGATGTCCGACCTG
CCGGAGGTGCGGAATGGAGCCTCGGCACTGGAGATCTCCTACAACGGGGA
GCCTCAGTCCTGGTTCCAGAGGCAGCTCCGGGGCGCACAGTACCAGCCCA
TCCTCAGGGACCACATCTGTAAGGACATGAGTGCATTGGTGGCTGCCCGC
ATGCGGCACATCCCCTTGGCCCCAGGGTCAGACTGGCGCGATCTGCCCAA
CATCGAGGTGCGGCTCTCAGACGGCACCATGGCCAGGAAGCTGCGGTATA
CCCACCATGACAGGAAGAACGGCCGCAGCAGCTCTGGGGCCCTCCGTGGG
GTCTGCTCCTGCGTGGAAGCCGGCAAAGCCTGCGACCCCGCAGCCAGGCA
GTTCAACACCCTCATCCCCTGGTGCCTGCCCCACACCGGGAACCGGCACA
ACCACTGGGCTGGCCTCTATGGAAGGCTCGAGTGGGACGGCTTCTTCAGC
ACAACCGTCACCAACCCCGAGCCCATGGGCAAGCAGGGCCGCGTGCTCCA
CCCAGAGCAGCACCGTGTGGTGAGCGTGCGGGAGTGTGCCCGCTCCCAGG
GCTTCCCTGACACCTACCGGCTCTTCGGCAACATCCTGGACAAGCACCGG
CAGGTGGGCAATGCCGTGCCACCGCCCCTGGCCAAAGCCATTGGCTTGGA
GATCAAGCTTTGTATGTTGGCCAAAGCCCGAGAGAGTGCCTCAGCTAAAA
TAAAGGAGGAGGAAGCTGCTAAGGACTAGgcggccgc

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU23865
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 5' Not1 frame 2. two silent mutations compared to NCBI entry at a1389g (silent at Pro463) and a1782g (silent at Thr594)