cDNA Clone SLX4

• Synonyms: BTBD12
• Plasmid: pBabe.puro.GFP.DU HA SLX4 sprot
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-HA SLX4 sprot
• Genbank: NM_032444.2 GI:63252862 with changes: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, c2454t, t4500c. Also sprot changes.
• Tag: GFP
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCACCATGTACCCATACGATGTGCCAGATTACGCCaaactgagtgt
gaatgaggctcagctaggcttctacttgggttcactttctcatctgtctg
cctgtcctgggattgaccctcgctcctctgaagaccagcctgaaagcctt
aaaactggtcagatgatggatgagtctgatgaggactttaaagaactctg
cgctagctttttccaaagggtgaaaaaacatggaatcaaggaagtgtcag
gagaaaggaagacacaaaaggctgcctcaaacggcactcagataagaagc
aaattgaaaaggaccaaacaaactgctaccaagaccaaaacccttcaagg
ccctgcagagaagaaacctccgtctggcagccaggcccctaggactaaaa
agcaaagggtaaccaaatggcaagcaagtgaaccggcccactctgtgaat
ggggaggggggtgtgcttgcctctgctccagatccacctgtgctccggga
aacagcacaaaacacccagacgggtaaccagcaagaaccatcgccaaacc
tttccagagagaaaaccagagagaatgtgcccaacagcgactcccagcct
cctccttcctgtttgacaacagcagtgccaagtccctccaaaccccgcac
agcacaattggtcctacagcgaatgcagcagttcaagagagcagaccccg
agcgtttgagacacgcttcagaagagtgctccctcgaggctgcgcgggaa
gaaaatgtcccaaaggaccctcaagaggagatgatggcggggaatgtgta
tgggcttgggccccctgccccagagagcgacgctgcggtggccttgaccc
tgcagcaggagtttgcacgggtaggagcatcggcacatgatgatagcctg
gaggaaaagggtttgttcttctgccagatttgtcaaaagaacctctcagc
catgaacgtgacccgaagggaacagcatgtgaacaggtgcttggatgaag
ctgaaaagacactaagaccttctgtgcctcagatccctgagtgcccgatt
tgtgggaaaccgtttcttaccttaaagagcagaaccagtcacttgaagca
gtgtgctgtgaagatggaggttggcccccagctcctgcttcaggctgtgc
ggctgcagacagcacagcctgagggtagcagcagcccacccatgttcagc
ttcagtgatcacagtagaggtctgaaacggagaggacccaccagcaagaa
ggagccacggaagaggcggaaggtggacgaggcaccgtccgaggacctgc
tggtggccatggctctgtcccggtcggagatggagccgggtgcggctgta
ccagcgctcaggctggaaagtgccttttctgagaggataagaccagaagc
agagaataaaagtcgcaagaagaaacccccggtatcccccccattgttgt
tagtccaggactctgaaaccacaggccgacagatagaggaccgtgtggcc
ctgctcctctctgaggaagtggaattgtctagcacgccaccacttcctgc
cagcaggattttaaaggaagggtgggaaagagcgggccagtgtcctcctc
cacctgaacgcaagcagagctttctgtgggagggcagcgcactgactggg
gcctgggccatggaggacttctacacggccaggctggtccctcctctcgt
gccccagcggcctgcccagggccttatgcaggagcccgtgccgcctctgg
tgccacctgagcactcagagctgagcgagcgaaggtcacccgctctccac
ggcacccccactgcaggctgtggctccaggggcccgtcgccttcggccag
ccagagggagcaccaggccctgcaggacctcgtggacctggcgagggagg
gactgagcgccagcccgtggcccggcagtgggggcctggctggctcggaa
gggactgcagggttggacgtggtgcccggcggccttcctctgactgggtt
tgtggtgccatcgcaggacaagcacccggacaggggcggccgaaccttgc
tctccctcgggctgctggttgctgactttggcgccatggtcaataaccca
cacctgagtgatgtccagtttcagacggacagcggggaggtgctttacgc
ccacaagttcgtgctttatgcccgatgcccgctcctcatccagtatgtga
acaatgaaggcttctccgctgtagaggacggggttctgacccagcgtgtc
ctgctgggtgacgtgagcaccgaggccgcccgcacgttcctgcactatct
ctacactgcggacactggccttcctcctggccttagctctgagctgagct
ccctggcccacaggtttggcgtgagtgagctcgttcacctgtgcgaacag
gtgcctattgccactgactcagagggcaaaccatgggaggagaaggaagc
agagaattgcgaaagcagggccgagaatttccaggaacttttgaggtcaa
tgtgggcagatgaagaggaggaagcggagactttgttgaaatccaaggac
cacgaagaagatcaagaaaacgtaaacgaggcagagatggaagaaattta
tgaatttgcagctactcagcgaaagcttctccaggaagaaagggcagcgg
gtgccggcgaggacgctgactggctggagggtggcagtccggtttctggg
caactcctagcaggtgtccaggtgcagaaacagtgggacaaggtggagga
gatggagccgttggagccaggaagagatgaggccgccaccacctgggaga
agatgggacagtgcgctctcccgccaccccagggccagcactcaggggca
cggggagcagaggcccctgagcaggaggcgccagaggaggcgcttggcca
ttccagctgctccagcccttccagggactgccaggcagagagaaaagaag
gctctcttccgcactcagatgatgccggggattacgaacagctcttctca
tcaactcagggagagatctcagagccgtcccaaataacaagtgagcccga
ggaacaaagtggcgctgtcagggaaagggggctggaggtttctcatcgcc
tggctccctggcaggcatctccaccgcacccgtgccgcttcctattgggg
cctccccagggcgggagtccccgcgggtctcatcacacaagtgggtcgtc
cctgtcaacaccccggtcccgtggcggaacttcccaggtgggctccccaa
ccttgctgtctccagctgtgccatcaaagcagaaaagggacaggagcatc
ctcacgctgtctaaagagccagggcaccagaaaggcaaagagcgtcggtc
cgtgctggagtgcagaaataagggggtcctgatgttcccagaaaaatctc
tgtctattgacctaacccagtcaaatcctgaccattcgagctccagatct
cagaaatcttcatccaaactgaacgaagaagatgaggtcatcctcttact
ggactcggatgaggagctggagctagaacaaaccaaaatgaagtccattt
ctagtgatcctctggaagaaaagaaagctctagaaattagccctaggtcc
tgtgagctgttttccatcattgatgttgatgcagatcaggaaccttccca
gagcccaccaagaagcgaagctgtgctgcagcaggaggatgagggggcgc
tgccggagaatcggggctctttgggcaggagaggggctccctggctgttc
tgtgaccgtgagagcagccccagcgaggccagcaccacagacacctcgtg
gctggtgcccgccaccccgctggccagcagaagccgtgactgttcttccc
agacccaaatcagcagcctcaggagcgggctggccgtgcaggcggtgact
cagcacacgcccagggcctcagtaggaaacagggaagggaacgaagtcgc
acagaagttttctgtcatcaggccccagacaccaccgccccagacaccgt
cctcatgcctcactcccgtctctccaggaacttctgacggcagaaggcaa
ggccacagaagcccttcccgtccccaccccgggggccacccgcactcctc
tccgctggctccacatcccatctcaggggaccgcgcccacttcagcaggc
ggttcctgaaacactcgccgcctgggccaagcttcctgaaccagacccca
gcgggtgaagtggtggaagtcggagacagtgacgatgagcaggaggtggc
ctcccatcaggccaacagaagccccccactggacagtgaccccccaattc
caattgacgactgctgctggcacatggagcccctctcgccaattcccatt
gaccactggaacctggagcggaccggccccctgagcaccagcagccccag
ccgcaggatgaacgaggccgccgacagccgtgactgtcgctccccgggac
tcctggacaccacccccatccgaggaagctgcactacccagaggaaattg
caagagaagtcctcgggcgcgggctccctggggaacagcaggccgagctt
tctgaattcggctctgtgggacgtttgggacggggaagagcagaggcctc
cagagacccctcctccggcccagatgccaagcgctggtggagctcagaag
cccgaagggttagagacacccaaaggtgctaatcggaagaagaacttgcc
ccccaaagtgcccataacgccgatgccacagtattccattatggagacgc
cggtgctgaagaaggaactggataggtttggagtccgccctctgcctaaa
cgccagatggttctgaagctgaaggagatattccagtacactcaccagac
cctggactcagactccgaggacgagagccagtcctcacagccgctgttgc
aggcgcctcactgccagaccctcgcctcccagacctacaagccttcaagg
gcaggggtccatgcccagcaggaggccaccacaggacctggggcccatag
gcccaagggacctgctaagaccaagggcccccgacatcaaaggaagcatc
atgaaagcatcacacccccaagcaggtcgcccaccaaggaggcacctcca
ggcctcaatgatgacgcccagatcccagcctctcaagaatccgtggccac
ctctgtggatggcagtgacagctccttgagctcacagagttcttcctcct
gtgagtttggagcggcatttgagtctgcaggtgaagaggagggcgagggg
gaggtcagtgcctcgcaggcagccgtgcaggcggcggacacagacgaggc
gctgaggtgctacatccgctccaagccggccctgtaccagaaggtgctgc
tgtaccagccctttgagctgcgggagctgcaggcagagctgaggcagaac
ggcctccgtgtgtcctcgcgcaggctgttggacttcctggacacccactg
tatcaccttcaccactgccgccacccgcagggagaagctccagggcagga
ggcggcagcctcggggcaagaagaaggtggagcggaactgagcggccgc

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSTMYPY
DVPDYAKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMD
ESDEDFKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQ
TATKTKTLQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLA
SAPDPPVLRETAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTT
AVPSPSKPRTAQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDP
QEEMMAGNVYGLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFF
CQICQKNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLT
LKSRTSHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRG
LKRRGPTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLES
AFSERIRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEV
ELSSTPPLPASRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDF
YTARLVPPLVPQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGC
GSRGPSPSASQREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDV
VPGGLPLTGFVVPSQDKHPDRGGRTLLSLGLLVADFGAMVNNPHLSDVQF
QTDSGEVLYAHKFVLYARCPLLIQYVNNEGFSAVEDGVLTQRVLLGDVST
EAARTFLHYLYTADTGLPPGLSSELSSLAHRFGVSELVHLCEQVPIATDS
EGKPWEEKEAENCESRAENFQELLRSMWADEEEEAETLLKSKDHEEDQEN
VNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQ
VQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPE
QEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEIS
EPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSP
RGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEP
GHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKL
NEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSII
DVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSP
SEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRAS
VGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSR
PHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEV
GDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLER
TGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGA
GSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETP
KGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKL
KEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQ
EATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQ
IPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQA
AVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSR
RLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45382
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.