cDNA Clone SLX4

• Synonyms: BTBD12
• Plasmid: pBabe.puro.GFP.DU SLX4 sprot H1020A L1022A P1024A
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-SLX4 sprot H1020A L1022A P1024A
• Genbank: NM_032444.2 GI:63252862 with changes: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, c2454t, t4500c. Also sprot changes.
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccatgaaactgagtgtgaatgaggctcagctaggcttctacttggg
ttcactttctcatctgtctgcctgtcctgggattgaccctcgctcctctg
aagaccagcctgaaagccttaaaactggtcagatgatggatgagtctgat
gaggactttaaagaactctgcgctagctttttccaaagggtgaaaaaaca
tggaatcaaggaagtgtcaggagaaaggaagacacaaaaggctgcctcaa
acggcactcagataagaagcaaattgaaaaggaccaaacaaactgctacc
aagaccaaaacccttcaaggccctgcagagaagaaacctccgtctggcag
ccaggcccctaggactaaaaagcaaagggtaaccaaatggcaagcaagtg
aaccggcccactctgtgaatggggaggggggtgtgcttgcctctgctcca
gatccacctgtgctccgggaaacagcacaaaacacccagacgggtaacca
gcaagaaccatcgccaaacctttccagagagaaaaccagagagaatgtgc
ccaacagcgactcccagcctcctccttcctgtttgacaacagcagtgcca
agtccctccaaaccccgcacagcacaattggtcctacagcgaatgcagca
gttcaagagagcagaccccgagcgtttgagacacgcttcagaagagtgct
ccctcgaggctgcgcgggaagaaaatgtcccaaaggaccctcaagaggag
atgatggcggggaatgtgtatgggcttgggccccctgccccagagagcga
cgctgcggtggccttgaccctgcagcaggagtttgcacgggtaggagcat
cggcacatgatgatagcctggaggaaaagggtttgttcttctgccagatt
tgtcaaaagaacctctcagccatgaacgtgacccgaagggaacagcatgt
gaacaggtgcttggatgaagctgaaaagacactaagaccttctgtgcctc
agatccctgagtgcccgatttgtgggaaaccgtttcttaccttaaagagc
agaaccagtcacttgaagcagtgtgctgtgaagatggaggttggccccca
gctcctgcttcaggctgtgcggctgcagacagcacagcctgagggtagca
gcagcccacccatgttcagcttcagtgatcacagtagaggtctgaaacgg
agaggacccaccagcaagaaggagccacggaagaggcggaaggtggacga
ggcaccgtccgaggacctgctggtggccatggctctgtcccggtcggaga
tggagccgggtgcggctgtaccagcgctcaggctggaaagtgccttttct
gagaggataagaccagaagcagagaataaaagtcgcaagaagaaaccccc
ggtatcccccccattgttgttagtccaggactctgaaaccacaggccgac
agatagaggaccgtgtggccctgctcctctctgaggaagtggaattgtct
agcacgccaccacttcctgccagcaggattttaaaggaagggtgggaaag
agcgggccagtgtcctcctccacctgaacgcaagcagagctttctgtggg
agggcagcgcactgactggggcctgggccatggaggacttctacacggcc
aggctggtccctcctctcgtgccccagcggcctgcccagggccttatgca
ggagcccgtgccgcctctggtgccacctgagcactcagagctgagcgagc
gaaggtcacccgctctccacggcacccccactgcaggctgtggctccagg
ggcccgtcgccttcggccagccagagggagcaccaggccctgcaggacct
cgtggacctggcgagggagggactgagcgccagcccgtggcccggcagtg
ggggcctggctggctcggaagggactgcagggttggacgtggtgcccggc
ggccttcctctgactgggtttgtggtgccatcgcaggacaagcacccgga
caggggcggccgaaccttgctctccctcgggctgctggttgctgactttg
gcgccatggtcaataacccacacctgagtgatgtccagtttcagacggac
agcggggaggtgctttacgcccacaagttcgtgctttatgcccgatgccc
gctcctcatccagtatgtgaacaatgaaggcttctccgctgtagaggacg
gggttctgacccagcgtgtcctgctgggtgacgtgagcaccgaggccgcc
cgcacgttcctgcactatctctacactgcggacactggccttcctcctgg
ccttagctctgagctgagctccctggcccacaggtttggcgtgagtgagc
tcgttcacctgtgcgaacaggtgcctattgccactgactcagagggcaaa
ccatgggaggagaaggaagcagagaattgcgaaagcagggccgagaattt
ccaggaacttttgaggtcaatgtgggcagatgaagaggaggaagcggaga
ctttgttgaaatccaaggaccacgaagaagatcaagaaaacgtaaacgag
gcagagatggaagaaatttatgaatttgcagctactcagcgaaagcttct
ccaggaagaaagggcagcgggtgccggcgaggacgctgactggctggagg
gtggcagtccggtttctgggcaactcctagcaggtgtccaggtgcagaaa
cagtgggacaaggtggaggagatggagccgttggagccaggaagagatga
ggccgccaccacctgggagaagatgggacagtgcgctctcccgccacccc
agggccagcactcaggggcacggggagcagaggcccctgagcaggaggcg
ccagaggaggcgcttggccattccagctgctccagcccttccagggactg
ccaggcagagagaaaagaaggctctcttccgcactcagatgatgccgggg
attacgaacagctcttctcatcaactcagggagagatctcagagccgtcc
caaataacaagtgagcccgaggaacaaagtggcgctgtcagggaaagggg
gctggaggtttctgctcgccgggctgcctggcaggcatctccaccgcacc
cgtgccgcttcctattggggcctccccagggcgggagtccccgcgggtct
catcacacaagtgggtcgtccctgtcaacaccccggtcccgtggcggaac
ttcccaggtgggctccccaaccttgctgtctccagctgtgccatcaaagc
agaaaagggacaggagcatcctcacgctgtctaaagagccagggcaccag
aaaggcaaagagcgtcggtccgtgctggagtgcagaaataagggggtcct
gatgttcccagaaaaatctctgtctattgacctaacccagtcaaatcctg
accattcgagctccagatctcagaaatcttcatccaaactgaacgaagaa
gatgaggtcatcctcttactggactcggatgaggagctggagctagaaca
aaccaaaatgaagtccatttctagtgatcctctggaagaaaagaaagctc
tagaaattagccctaggtcctgtgagctgttttccatcattgatgttgat
gcagatcaggaaccttcccagagcccaccaagaagcgaagctgtgctgca
gcaggaggatgagggggcgctgccggagaatcggggctctttgggcagga
gaggggctccctggctgttctgtgaccgtgagagcagccccagcgaggcc
agcaccacagacacctcgtggctggtgcccgccaccccgctggccagcag
aagccgtgactgttcttcccagacccaaatcagcagcctcaggagcgggc
tggccgtgcaggcggtgactcagcacacgcccagggcctcagtaggaaac
agggaagggaacgaagtcgcacagaagttttctgtcatcaggccccagac
accaccgccccagacaccgtcctcatgcctcactcccgtctctccaggaa
cttctgacggcagaaggcaaggccacagaagcccttcccgtccccacccc
gggggccacccgcactcctctccgctggctccacatcccatctcagggga
ccgcgcccacttcagcaggcggttcctgaaacactcgccgcctgggccaa
gcttcctgaaccagaccccagcgggtgaagtggtggaagtcggagacagt
gacgatgagcaggaggtggcctcccatcaggccaacagaagccccccact
ggacagtgaccccccaattccaattgacgactgctgctggcacatggagc
ccctctcgccaattcccattgaccactggaacctggagcggaccggcccc
ctgagcaccagcagccccagccgcaggatgaacgaggccgccgacagccg
tgactgtcgctccccgggactcctggacaccacccccatccgaggaagct
gcactacccagaggaaattgcaagagaagtcctcgggcgcgggctccctg
gggaacagcaggccgagctttctgaattcggctctgtgggacgtttggga
cggggaagagcagaggcctccagagacccctcctccggcccagatgccaa
gcgctggtggagctcagaagcccgaagggttagagacacccaaaggtgct
aatcggaagaagaacttgccccccaaagtgcccataacgccgatgccaca
gtattccattatggagacgccggtgctgaagaaggaactggataggtttg
gagtccgccctctgcctaaacgccagatggttctgaagctgaaggagata
ttccagtacactcaccagaccctggactcagactccgaggacgagagcca
gtcctcacagccgctgttgcaggcgcctcactgccagaccctcgcctccc
agacctacaagccttcaagggcaggggtccatgcccagcaggaggccacc
acaggacctggggcccataggcccaagggacctgctaagaccaagggccc
ccgacatcaaaggaagcatcatgaaagcatcacacccccaagcaggtcgc
ccaccaaggaggcacctccaggcctcaatgatgacgcccagatcccagcc
tctcaagaatccgtggccacctctgtggatggcagtgacagctccttgag
ctcacagagttcttcctcctgtgagtttggagcggcatttgagtctgcag
gtgaagaggagggcgagggggaggtcagtgcctcgcaggcagccgtgcag
gcggcggacacagacgaggcgctgaggtgctacatccgctccaagccggc
cctgtaccagaaggtgctgctgtaccagccctttgagctgcgggagctgc
aggcagagctgaggcagaacggcctccgtgtgtcctcgcgcaggctgttg
gacttcctggacacccactgtatcaccttcaccactgccgccacccgcag
ggagaagctccagggcaggaggcggcagcctcggggcaagaagaaggtgg
agcggaactgagcggccgc

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKLSV
NEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELC
ASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQG
PAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRE
TAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRT
AQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVY
GLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSA
MNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQ
CAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKK
EPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEA
ENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPA
SRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLV
PQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSAS
QREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGF
VVPSQDKHPDRGGRTLLSLGLLVADFGAMVNNPHLSDVQFQTDSGEVLYA
HKFVLYARCPLLIQYVNNEGFSAVEDGVLTQRVLLGDVSTEAARTFLHYL
YTADTGLPPGLSSELSSLAHRFGVSELVHLCEQVPIATDSEGKPWEEKEA
ENCESRAENFQELLRSMWADEEEEAETLLKSKDHEEDQENVNEAEMEEIY
EFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQWDKVEE
MEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPEEALGH
SSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEISEPSQITSEPE
EQSGAVRERGLEVSARRAAWQASPPHPCRFLLGPPQGGSPRGSHHTSGSS
LSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEPGHQKGKERRS
VLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKLNEEDEVILLL
DSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDADQEPSQ
SPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEASTTDTSW
LVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNREGNEVA
QKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSRPHPGGHPHSS
PLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEVGDSDDEQEVA
SHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLERTGPLSTSSPS
RRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGAGSLGNSRPSF
LNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANRKKNLP
PKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQT
LDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAHR
PKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVAT
SVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDEA
LRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTHC
ITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45383
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.