cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pCMV5 HA TTBK2 ko bam
• Parent Plasmid: pCMV5 HA2
• Expressed: HA-TTBK2
• Genbank: NM_173500.3
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGAGTGGGGGAGGAGAGCAGCCGGATATCCTGAGTGTTGG
AATCCTAGTGAAAGAAAGATGGAAAGTGTTGAGAAAGATTGGGGGTGGGG
GCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAAAATGTT
GCACTGAAGGTGGAATCAGCTCAACAACCAAAACAAGTTCTGAAAATGGA
AGTTGCTGTTTTGAAAAAGCTGCAAGGGAAAGACCATGTTTGTAGATTTA
TTGGCTGTGGGAGGAATGATCGATTCAACTATGTGGTCATGCAGTTGCAG
GGTCGGAATCTGGCAGATCTTCGCCGTAGCCAGTCCCGAGGCACATTCAC
CATTAGTACCACTCTCCGGCTGGGTAGACAGATTTTGGAGTCTATTGAAA
GCATTCATTCTGTGGGATTCTTGCATCGAGACATCAAACCGTCGAACTTC
GCTATGGGTCGCTTTCCTAGTACATGTAGGAAATGTTACATGCTTGATTT
TGGCTTGGCTCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTC
GAGCTGTGGCAGGTTTTCGAGGGACAGTTCGTTATGCATCAATCAACGCA
CATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCCTTATTCTA
CATGTTGGTGGAGTTTGTGGTTGGTCAGCTGCCCTGGAGAAAAATAAAGG
ACAAGGAGCAAGTAGGCTCTATTAAGGAGAGATATGACCACAGGCTCATG
TTGAAACATCTCCCTCCAGAATTCAGCATCTTTCTAGACCATATCTCTTC
TTTGGATTATTTTACAAAACCAGACTACCAGCTTCTTACATCCGTGTTTG
ACAATAGCATCAAGACTTTTGGAGTAATTGAGAGTGACCCTTTTGACTGG
GAGAAGACTGGAAATGATGGCTCCCTAACAACCACCACTACTTCTACCAC
CCCTCAGTTGCACACTCGCTTGACCCCTGCTGCAATTGGAATTGCCAATG
CTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATACAGATGAGGTATTT
CCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCCCTGTTGGTGTGTC
ACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGTCCCCAGGAGAAGG
ATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGATAAAGCTTGGAATT
TGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCCAGGCAAATGGTCT
TCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTCCGCTCAGAGATTA
CTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTTACGTTCCATTCAC
AGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAAAGCCAGACACTGA
CAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAAGATACCAGTGCAG
GAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCCTTGCGTTCCTGCT
GTGTCCCGTACTGACCACATCTGGCACTATGATGAAGAATATCTTCCAGA
TGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAGGCAGATGGTGGTG
GCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTGCAAGCAAGAAATT
GATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGGACCTTCAGGATTT
TAGGACAAATGAGGCTGTAGGACATAAAACAACTGGAAGTCCTTCTGATG
AGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACCTCAAGATGAAAAG
CTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTC
AGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAG
AACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTT
ATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCT
TACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTT
CAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCC
ATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTT
GGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGG
GGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAA
AGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAG
ACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAA
GCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAG
CATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAA
GGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTT
TTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTG
ACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGA
AGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGG
ATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCT
ACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGG
AGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGA
AGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTT
TTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGC
AAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGC
TTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGT
GCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTT
TTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTC
ACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAG
TCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCA
GGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAA
AGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTC
CTAGGGAGTAGTAACTCCGACTCAGACCTTTTCTCCCGCCTGGCCCAAAT
TCTTCAAAATGGATCTCAGAAACCCCGGAGCACTACTCAGTGCAAGAGTC
CAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTCCCT
CGCAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGCACGTC
TAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTT
CGTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCT
TCCTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAA
TGGCCTGAAAGGCTCCGGCAGCCTCCACCACCACTCAGCCAGCACTAAAA
CCCCCCAAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGATAGCGGCCG
C

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMSGGGEQPDILSVGILVKERWKVLRKI
GGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHV
CRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILE
SIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDV
RPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWR
KIKDKEQVGSIKERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLT
SVFDNSIKTFGVIESDPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIG
IANATPIPGDLLRENTDEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRP
QEKDVWEEMDANKNKIKLGICKAATEEENSHGQANGLLNAPSLGSPIRVR
SEITQPDRDIPLVRKLRSIHSFELEKRLTLEPKPDTDKFLETCLEKMQKD
TSAGKESILPALLHKPCVPAVSRTDHIWHYDEEYLPDASKPASANTPEQA
DGGGSNGFIAVNLSSCKQEIDSKEWVIVDKEQDLQDFRTNEAVGHKTTGS
PSDEEPEVLQVLEASPQDEKLQLGPWAENDHLKKETSGVVLALSAEGPPT
AASEQYTDRLELQPGAASQFIAATPTSLMEAQAEGPLTAITIPRPSVAST
QSTSGSFHCGQQPEKKDLQPMEPTVELYSPRENFSGLVVTEGEPPSGGSR
TDLGLQIDHIGHDMLPNIRESNKSQDLGPKELPDHNRLVVREFENLPGET
EEKSILLESDNEDEKLSRGQHCIEISSLPGDLVIVEKDHSATTEPLDVTK
TQTFSVVPNQDKNNEIMKLLTVGTSEISSRDIDPHVEGQIGQVAEMQKNK
ISKDDDIMSEDLPGHQGDLSTFLHQEGKREKITPRNGELFHCVSENEHGA
PTRKDMVRSSFVTRHSRIPVLAQEIDSTLESSSPVSAKEKLLQKKAYQPD
LVKLLVEKRQFKSFLGDLSSASDKLLEEKLATVPAPFCEEEVLTPFSRLT
VDSHLSRSAEDSFLSPIISQSRKSKIPRPVSWVNTDQVNSSTSSQFFPRP
PPGKPPTRPGVEARLRRYKVLGSSNSDSDLFSRLAQILQNGSQKPRSTTQ
CKSPGSPHNPKTPPKSPVVPRRSPSASPRSSSLPRTSSSSPSRAGRPHHD
QRSSSPHLGRSKSPPSHSGSSSSRRSCQQEHCKPSKNGLKGSGSLHHHSA
STKTPQGKSKPASKLSR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44439
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: TTBK2 is difficult to shuttle due to many commonly used restriction sites being present. Have knocked out the internal BamHI site to facilitate directional cloning work with this gene. Template only, will be reamplified as BamHI/NotI insert.