cDNA Clone HA-HPK1-FLAG

• Plasmid: pRETROX Tight HA HPK1 FLAG
• Parent Plasmid: pRetroX tight puro
• Expressed: HA HPK1 FLAG
• Genbank: NP_001036065.1
• Tag: HA
• Tag: FLAG

Sequence of Insert

GGCGGCCGCGGCCACCATGTACCCATACGATGTGCCAGATTACGCCatgg
acgtcgtggaccctgacattttcaatagagacccccgggaccactatgac
ctgctacagcggctgggtggcggcacgtatggggaagtctttaaggctcg
agacaaggtgtcaggggacctggtggcactgaagatggtgaagatggagc
ctgatgatgatgtctccacccttcagaaggaaatcctcatattgaaaact
tgccggcacgccaacatcgtggcctaccatgggagttatctctggttgca
gaaactctggatctgcatggaattctgtggggctggttctctccaggaca
tctaccaagtgacaggctccctgtcagagctccagattagctatgtctgc
cgggaagtgctccagggactggcctatttgcactcacagaagaagataca
cagggacatcaagggagctaacatcctcatcaatgatgctggggaggtca
gattggctgactttggcatctcggcccagattggggctacactggccaga
cgcctctctttcattgggacaccctactggatggctccggaagtggcagc
tgtggccctgaagggaggatacaatgagctgtgtgacatctggtccctgg
gcatcacggccatcgaactggccgagctacagccaccgctctttgatgtg
caccctctcagagttctcttcctcatgaccaagagtggctaccagcctcc
ccgactgaaggaaaaaggcaaatggtcggctgccttccacaacttcatca
aagtcactctgactaagagtcccaagaaacgacccagcgccaccaagatg
ctcagtcatcaactggtatcccagcctgggctgaatcgaggcctgatcct
ggatcttcttgacaaactgaagaatcccgggaaaggaccctccattgggg
acattgaggatgaggagcccgagctaccccctgctatccctcggcggatc
agatccacccaccgctccagctctctggggatcccagatgcagactgctg
tcggcggcacatggagttcaggaagctccgaggaatggagaccagacccc
cagccaacaccgctcgcctacagcctcctcgagacctcaggagcagcagc
cccaggaagcaactgtcagagtcgtctgacgatgactatgacgacgtgga
catccccacccctgcagaggacacacctcctccacttccccccaagccca
agttccgttctccatcagacgagggtcctgggagcatgggggatgatggg
cagctgagcccgggggtgctggtccggtgtgccagtgggcccccaccaaa
cagcccccgtcctgggcctcccccatccaccagcagcccccacctcaccg
cccattcagaaccctcactctggaacccaccctcccgggagcttgacaag
cccccacttctgccccccaagaaggaaaagatgaagagaaagggatgtgc
ccttctcgtaaagttgttcaatggctgccccctccggatccacagcacgg
ccgcctggacacatccctccaccaaggaccagcacctgctcctgggggca
gaggaaggcatctttatcctgaaccggaatgaccaggaggccacgctgga
aatgctctttcctagccggactacgtgggtgtactccatcaacaacgttc
tcatgtctctctcaggaaagaccccccacctgtattctcatagcatcctt
ggcctgctggaacggaaagagaccagagcaggaaaccccatcgctcacat
tagcccccaccgcctactggcaaggaagaacatggtttccaccaagatcc
aggacaccaaaggctgccgggcgtgctgtgtggcggagggtgcgagctct
gggggcccgttcctgtgcggtgcattggagacgtccgttgtcctgcttca
gtggtaccagcccatgaacaaattcctgcttgtccggcaggtgctgttcc
cactgccgacgcctctgtccgtgttcgcgctgctgaccgggccaggctct
gagctgcccgctgtgtgcatcggcgtgagccccgggcggccggggaagtc
ggtgctcttccacacggtgcgctttggcgcgctctcttgctggctgggcg
agatgagcaccgagcacaggggacccgtgcaggtgacccaggtagaggaa
gatatggtgatggtgttgatggatggctctgtgaagctggtgaccccgga
ggggtccccagtccggggacttcgcacacctgagatccccatgaccgaag
cggtggaggccgtggctatggttggaggtcagcttcaggccttctggaag
catggagtgcaggtgtgggctctaggctcggatcagctgctacaggagct
gagagaccctaccctcactttccgtctgcttggctcccccaggcctgtag
tggtggagacacgcccagtggatgatcctactgctcccagcaacctctac
atccaggaaGACTACAAGGACGATGACGATAAGTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAMDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSG
DLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWIC
MEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKG
ANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKG
GYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEK
GKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDK
LKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHME
FRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPA
EDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPG
PPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKL
FNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPS
RTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRL
LARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPM
NKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHT
VRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVR
GLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTL
TFRLLGSPRPVVVETRPVDDPTAPSNLYIQEDYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU43719
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please insert a FLAG tag at the C-terminus of the HA-HPK1 insert in pRETRO-X-Tight. Thanks!