cDNA Clone ITCH

• Plasmid: pET15b 6HIS TEV ITCH aa483-862
• Parent Plasmid: pET15b 6His TEV1
• Expressed: 6HIS-ITCH aa483-862
• Genbank: NM_031483
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccTATGTTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTG
GTGTCAGCAACTGGCCATGCCACAGCACATAAAGATTACAGTGACAAGAA
AAACATTGTTTGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAA
GATCTGCGAAGACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGA
TTATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGT
TGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGC
TTGCAGATAAACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTT
TCGTTTTATTGGCAGATTcATTGCCATGGCTCTGTTCCATGGGAAATTCA
TAGACACGGGTTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCA
GTTGGACTCAAGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCT
CATCTGGGTTAAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACT
TCTCCGTTGACAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAA
CCTAATGGTGGCAATATTCTTGTAACAGAAGAAAATAAAGAGGAATACAT
CAGAATGGTAGCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACAC
AAGCTTTCTTTGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAA
TACTTTGATGCAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGAT
TGATTTGAATGACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGA
CCAGCAAACAAATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAAT
GAGAAGAGAATGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCC
AGTAGGAGGATTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCT
GCATTGAAAAAGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGT
TTTAATCGCCTGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGA
AAAGCTGTTGTTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAAg
cggccgc

Amino Acid Sequence

MGSSHHHHHHSSGENLYFQGHMLGSYVRDFKAKVQYFRFWCQQLAMPQHI
KITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFF
LLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMA
LFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEE
CDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSR
GVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAI
YRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGS
NGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETE
GFGQE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23900
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent mutation compared to NCBI at Phe603