cDNA Clone EIF4E

• Synonyms: AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F
• Plasmid: pGEX6P-1 CASP3 EIF4E
• Parent Plasmid: pGEX6P1
• Expressed: GST-EIF4E
• Genbank: NM_001968.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCTGACGAGGTCGACATGGCGACTGTCGAACCGGAAACCACCCCTACTCCTAATCCCCCGACTACAGAAGAGGAGAAAACGGAATCTAATCAGGAGGTTGCTAACCCAGAACACTATATTAAACATCCCCTACAGAACAGATGGGCACTCTGGTTTTTTAAAAATGATAAAAGCAAAACTTGGCAAGCAAACCTGCGGCTGATCTCCAAGTTTGATACTGTTGAAGACTTTTGGGCTCTGTACAACCATATCCAGTTGTCTAGTAATTTAATGCCTGGCTGTGACTACTCACTTTTTAAGGATGGTATTGAGCCTATGTGGGAAGATGAGAAAAACAAACGGGGAGGACGATGGCTAATTACATTGAACAAACAGCAGAGACGAAGTGACCTCGATCGCTTTTGGCTAGAGACACTTCTGTGCCTTATTGGAGAATCTTTTGATGACTACAGTGATGATGTATGTGGCGCTGTTGTTAATGTTAGAGCTAAAGGTGATAAGATAGCAATATGGACTACTGAATGTGAAAACAGAGAAGCTGTTACACATATAGGGAGGGTATACAAGGAAAGGTTAGGACTTCCTCCAAAGATAGTGATTGGTTATCAGTCCCACGCAGACACAGCTACTAAGAGCGGCTCCACCACTAAAAATAGGTTTGTTGTTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDEVDMATVEPETTPTPNPP
TTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISK
FDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLI
TLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAI
WTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRF
VV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44452
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CASP3 cleavage site for analysis of blunt cleavage, this unfortunately appears twice in the GST tag and upon further analysis is more common than expected in the majority of proteins studied in-house. Further work on Casp3 discontinued, Precission site means this clone is still useful.