cDNA Clone SLX4 (BTBD12)

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO HaloP.DU SLX4 C296/299A
• Parent Plasmid: pcDNA5D FRT/TO Halo 3C
• Expressed: Halo-SLX4 C296/299A
• Genbank: NM_032444.2 GI:63252862 with changes: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, c2454t, t4500c.
• Tag: HALO
• Species: Human

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSMKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQM
MDESDEDFKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRT
KQTATKTKTLQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGV
LASAPDPPVLRETAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCL
TTAVPSPSKPRTAQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPK
DPQEEMMAGNVYGLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGL
FFAQIAQKNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPF
LTLKSRTSHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHS
RGLKRRGPTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRL
ESAFSERIRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSE
EVELSSTPPLPASRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAME
DFYTARLVPPLVPQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTA
GCGSRGPSPSASQREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGL
DVVPGGLPLTGFVVPSQDKHPDRGGRTLLSLGLLVADFGAMVNNPHLSDV
QFQTDSGEVLYAHKFVLYARCPLLIQYVNNEGFSAVEDGVLTQRVLLGDV
STEAARTFLHYLYTADTGLPPGLSSELSSLAHRFGVSELVHLCEQVPIAT
DSEGKPWEEKEAENCESRAENFQELLRSMWADEEEEAETLLKSKDHEEDQ
ENVNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAG
VQVQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEA
PEQEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGE
ISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGG
SPRGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSK
EPGHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSS
KLNEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFS
IIDVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRES
SPSEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPR
ASVGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSP
SRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVV
EVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNL
ERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSS
GAGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLE
TPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVL
KLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHA
QQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDD
AQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSAS
QAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVS
SRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45445
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.