cDNA Clone PP4

• Plasmid: pFastbac GST PP4C D85N
• Parent Plasmid: pFastbac GST
• Expressed: GST-PP4 D85N
• Genbank: NP_002711
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGAGATCAGCGACCTGGACCGGCAGATCGAGCAGCTGCG
TCGCTGCGAGCTCATCAAGGAGAGCGAAGTCAAGGCCCTGTGCGCTAAGG
CCAGAGAGATCTTGGTAGAGGAGAGCAACGTGCAGAGGGTGGACTCGCCA
GTCACAGTGTGCGGCGACATCCATGGACAATTCTATGACCTCAAAGAGCT
GTTCAGAGTAGGTGGCGACGTCCCTGAGACCAACTACCTCTTCATGGGGG
ACTTTGTGAACCGTGGCTTCTATAGCGTCGAAACGTTCCTCCTGCTGCTG
GCACTTAAGGTTCGCTATCCTGATCGCATCACACTGATCCGGGGCAACCA
TGAGAGTCGCCAGATCACGCAGGTCTATGGCTTCTACGATGAGTGCCTGC
GCAAGTACGGCTCGGTGACTGTGTGGCGCTACTGCACTGAGATCTTTGAC
TACCTCAGCCTGTCAGCCATCATCGATGGCAAGATCTTCTGCGTGCACGG
GGGCCTCTCCCCCTCCATCCAGACCCTGGATCAGATTCGGACAATCGACC
GAAAGCAAGAGGTGCCTCATGATGGGCCCATGTGTGACCTCCTCTGGTCT
GACCCAGAAGACACCACAGGCTGGGGCGTGAGCCCCCGAGGAGCCGGCTA
CCTATTTGGCAGTGACGTGGTGGCCCAGTTCAACGCAGCCAATGACATTG
ACATGATCTGCCGTGCCCACCAACTGGTGATGGAAGGTTACAAGTGGCAC
TTCAACGAGACGGTGCTCACTGTGTGGTCGGCACCCAACTACTGCTACCG
CTGTGGGAATGTGGCAGCCATCTTGGAGCTGGACGAGCATCTCCAGAAAG
ATTTCATCATCTTTGAGGCTGCTCCCCAAGAGACACGGGGCATCCCCTCC
AAGAAGCCCGTGGCCGACTACTTCCTGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEISDLDRQIEQLRRCEL
IKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVG
GDVPETNYLFMGDFVNRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQ
ITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSP
SIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGS
DVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNV
AAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU43728
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST-PP4 D85N, we already have the wildtype