cDNA Clone RNMT

• Plasmid: pBMN-IRES-PURO (INI) RNMT-c-GFP
• Parent Plasmid: pBMN-IRES-PURO (INI)
• Expressed: RNMT-c-GFP
• Tag: GFP

Sequence of Insert

ATGGCAAATTCTGCAAAAGCAGAAGAATATGAAAAGATGTCTCTTGAACAGGCAAAAGCGTCAGTGAATTCTGAAACAGAGTCTTCATTCAATATTAATGAAAACACAACAGCTTCTGGGACTGGGCTTTCTGAAAAGACTTCTGTCTGTAGGCAAGTAGACATAGCAAGAAAGAGAAAAGAGTTTGAAGATGATCTTGTAAAGGAAAGTTCTAGTTGTGGGAAAGACACTCCATCCAAGAAGAGAAAACTTGATCCTGAAATTGTCCCAGAGGAAAAAGATTGTGGTGATGCTGAAGGCAATTCAAAGAAAAGAAAAAGAGAAACTGAGGATGTTCCAAAAGATAAATCTTCTACTGGAGATGGCACTCAAAATAAGAGAAAAATAGCACTTGAGGATGTTCCTGAAAAGCAGAAAAATCTGGAAGAAGGACACAGCTCAACAGTGGCTGCCCATTACAATGAACTTCAGGAAGTTGGTTTGGAGAAGCGTAGTCAAAGTCGTATTTTTTACCTAAGAAACTTTAATAATTGGATGAAAAGTGTTCTCATTGGAGAATTTTTGGAAAAGGTACGACAGAAGAAAAAACGTGATATCACTGTTTTGGACCTGGGATGTGGTAAAGGTGGAGATTTGCTGAAATGGAAAAAAGGAAGAATTAACAAGCTAGTTTGTACTGATATTGCCGATGTTTCTGTCAAACAGTGTCAGCAGCGGTATGAGGACATGAAAAATCGTCGTGATAGTGAATATATTTTCAGTGCAGAATTTATAACTGCTGACAGCTCAAAGGAACTTCTGATTGACAAATTTCGTGACCCACAAATGTGTTTTGACATCTGCAGTTGTCAGTTTGTCTGTCATTACTCATTTGAGTCTTATGAGCAGGCTGACATGATGCTGAGAAATGCGTGTGAGAGACTTAGCCCTGGGGGCTATTTTATTGGTACTACTCCCAATAGCTTTGAATTGATAAGACGCCTTGAAGCTTCAGAAACAGAATCATTTGGAAATGAAATATATACTGTGAAATTTCAGAAGAAAGGAGATTATCCTTTATTTGGCTGCAAATATGACTTCAACTTGGAAGGTGTTGTGGATGTTCCTGAATTCTTGGTCTATTTTCCATTGCTAAATGAAATGGCAAAGAAGTACAATATGAAACTAGTCTACAAAAAAACATTTCTGGAATTCTACGAAGAAAAGATTAAGAACAATGAAAATAAAATGCTCTTAAAACGAATGCAGGCCTTGGAGCCATATCCTGCAAATGAGAGTTCTAAACTTGTCTCTGAGAAGGTGGATGACTATGAACATGCAGCAAAGTACATGAAGAACAGTCAAGTAAGGTTACCTTTGGGAACCTTAAGTAAATCAGAATGGGAAGCTACAAGTATTTACTTGGTGTTTGCCTTTGAGAAACAGCAGGGTACCGCGGGCCCG

Amino Acid Sequence

MANSAKAEEYEKMSLEQAKASVNSETESSFNINENTTASGTGLSEKTSVC
RQVDIARKRKEFEDDLVKESSSCGKDTPSKKRKLDPEIVPEEKDCGDAEG
NSKKRKRETEDVPKDKSSTGDGTQNKRKIALEDVPEKQKNLEEGHSSTVA
AHYNELQEVGLEKRSQSRIFYLRNFNNWMKSVLIGEFLEKVRQKKKRDIT
VLDLGCGKGGDLLKWKKGRINKLVCTDIADVSVKQCQQRYEDMKNRRDSE
YIFSAEFITADSSKELLIDKFRDPQMCFDICSCQFVCHYSFESYEQADMM
LRNACERLSPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGD
YPLFGCKYDFNLEGVVDVPEFLVYFPLLNEMAKKYNMKLVYKKTFLEFYE
EKIKNNENKMLLKRMQALEPYPANESSKLVSEKVDDYEHAAKYMKNSQVR
LPLGTLSKSEWEATSIYLVFAFEKQQGTAGPGSIATMVSKGEELFTGVVP
ILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTT
LTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVK
FEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE
KRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42607
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: original RNMT-GFP cloned in BglII/NotI into BamHI/NotI.; ; Can use XhoI/BamHI to cut out RNMT without GFP and replace