cDNA Clone TRPM6

• Synonyms: CHAK2, HMGX, HOMG, HOMG1, HSH
• Plasmid: pCMV5 HA TRPM6 T1724E IRES GFP
• Parent Plasmid: pCMV5D HA
• Expressed: HA-TRPM6 (T1724E)-IRES-GFP
• Genbank: NM_017662.4
• Tag: HA
• Tag: GFP
• Species: Human

Sequence of Insert

GCTAGCGCCACCATGTACCCATACGACGTGCCAGACTACGCAAAAGAACA
ACCTGTCTTGGAGCGCTTGCAGTCCCAGAAATCCTGGATTAAAGGAGTAT
TTGACAAGAGAGAATGTAGCACAATCATACCCAGCTCAAAAAATCCTCAC
AGATGTACTCCAGTATGCCAAGTCTGCCAGAATTTAATCAGGTGTTACTG
TGGCCGACTGATTGGAGACCATGCTGGGATAGATTATTCCTGGACCATCT
CAGCTGCCAAGGGTAAAGAAAGTGAACAATGGTCTGTTGAAAAGCACACA
ACGAAAAGCCCAACAGATACTTTTGGCACGATTAATTTCCAAGATGGAGA
GCACACCCATCATGCCAAGTATATTAGAACTTCTTATGATACAAAACTGG
ATCATCTGTTACATTTAATGTTGAAAGAGTGGAAAATGGAACTGCCCAAG
CTTGTGATCTCAGTCCATGGGGGCATCCAGAACTTTACTATGCCCTCTAA
ATTTAAAGAGATTTTCAGCCAAGGTTTGGTTAAAGCTGCAGAGACAACCG
GTGCGTGGATAATAACTGAAGGCATCAATACAGGAGTGTCCAAGCATGTT
GGGGATGCCTTGAAATCCCATTCCTCTCATTCCTTGAGAAAAATCTGGAC
AGTTGGAATCCCTCCTTGGGGTGTCATTGAGAACCAGAGAGACCTTATTG
GAAAAGATGTGGTGTGCCTGTACCAGACTCTGGATAACCCCCTCAGCAAG
CTCACAACACTCAACAGCATGCACTCGCACTTCATCCTGTCTGATGATGG
GACCGTGGGCAAGTATGGAAATGAAATGAAGCTCAGAAGGAACCTGGAGA
AGTACCTCTCTCTGCAGAAAATACACTGCCGCTCAAGACAAGGCGTGCCG
GTCGTGGGGCTGGTGGTGGAAGGCGGTCCCAACGTCATCCTGTCAGTGTG
GGAGACTGTCAAGGACAAGGACCCAGTGGTGGTGTGTGAGGGTACAGGTA
GGGCGGCTGACCTCCTGGCCTTCACACACAAACACCTGGCAGATGAAGGG
ATGCTGCGACCTCAGGTGAAAGAGGAGATCATCTGCATGATTCAGAACAC
TTTCAACTTTAGTCTTAAACAGTCCAAGCACCTTTTCCAAATTCTAATGG
AGTGTATGGTTCACAGGGATTGTATTACCATATTTGATGCTGACTCTGAA
GAGCAGCAAGACCTGGACTTAGCAATCCTAACAGCTTTGCTGAAGGGCAC
AAATTTATCAGCGTCAGAGCAATTAAATCTGGCAATGGCTTGGGACAGGG
TGGACATTGCCAAGAAACATATCCTAATTTATGAACAACACTGGAAGCCT
GATGCCCTGGAACAAGCAATGTCAGATGCTTTAGTGATGGATCGGGTGGA
TTTTGTGAAGCTCTTAATAGAATATGGAGTGAACCTCCATCGCTTTCTTA
CCATCCCTCGACTGGAAGAGCTCTACAATACAAAACAAGGACCTACTAAT
ACACTCTTGCATCATCTCGTCCAAGATGTGAAACAGCATACCCTTCTTTC
AGGCTACCGAATAACCTTGATTGACATTGGATTAGTAGTAGAATACCTCA
TTGGTAGAGCATATCGCAGCAACTACACTAGAAAACATTTCAGAGCCCTC
TACAACAACCTCTACAGAAAATACAAGCACCAGAGACACTCCTCAGGAAA
TAGAAATGAGTCTGCAGAAAGTACGCTGCACTCCCAGTTCATTAGAACTG
CACAGCCATACAAATTCAAGGAAAAGTCTATAGTCCTTCATAAATCAAGG
AAGAAGTCAAAAGAACAAAATGTATCAGATGACCCTGAGTCTACTGGCTT
TCTTTACCCTTACAATGACCTGCTGGTTTGGGCTGTGCTGATGAAAAGGC
AGAAGATGGCTATGTTCTTCTGGCAGCATGGAGAGGAGGCCACGGTTAAA
GCCGTGATTGCGTGTATCCTCTACCGGGCAATGGCCCATGAAGCTAAGGA
GAGTCACATGGTGGATGATGCCTCAGAAGAGTTGAAGAATTACTCAAAAC
AGTTTGGCCAGCTGGCTCTGGACTTGTTGGAGAAGGCATTCAAGCAGAAT
GAGCGCATGGCCATGACGCTGTTGACGTATGAACTCAGGAACTGGAGCAA
TTCGACCTGCCTTAAACTGGCCGTGTCGGGAGGATTACGACCCTTTGTTT
CACATACTTGTACCCAGATGCTACTGACAGACATGTGGATGGGGAGGCTG
AAAATGAGGAAAAACTCTTGGTTAAAGATTATTATAAGCATTATTTTACC
ACCCACCATTTTGACACTGGAATTTAAAAGCAAAGCTGAGATGTCACATG
TTCCCCAGTCCCAGGACTTCCAATTTATGTGGTATTACAGTGACCAGAAC
GCCAGCAGTTCCAAAGAAAGTGCTTCTGTGAAAGAGTATGATTTGGAAAG
GGGCCATGATGAGAAACTGGATGAAAATCAGCATTTTGGTTTGGAAAGTG
GGCACCAACACCTTCCGTGGACCAGGAAAGTCTATGAGTTCTACAGTGCT
CCAATTGTCAAGTTTTGGTTTTATACGATGGCGTATTTGGCATTCCTCAT
GCTGTTCACTTACACCGTGTTGGTGGAGATGCAGCCCCAGCCCAGCGTGC
AGGAGTGGCTTGTTAGCATTTACATCTTCACCAATGCTATTGAGGTGGTC
AGGGAGATCTGTATTTCAGAACCTGGGAAGTTTACCCAAAAGGTGAAGGT
ATGGATTAGTGAGTACTGGAACTTAACAGAAACTGTGGCCATTGGCCTGT
TTTCAGCTGGCTTCGTCCTTCGATGGGGTGACCCTCCTTTTCACACAGCG
GGAAGACTGATCTACTGCATAGACATCATATTCTGGTTCTCACGGCTCCT
GGACTTCTTTGCTGTGAATCAACATGCAGGTCCATATGTGACCATGATTG
CAAAAATGACAGCAAACATGTTCTATATTGTGATCATCATGGCCATAGTC
CTGCTGAGCTTTGGAGTGGCACGCAAGGCCATCCTTTCGCCAAAAGAGCC
ACCATCTTGGAGTCTAGCTCGAGATATTGTATTTGAGCCATACTGGATGA
TATACGGAGAAGTCTATGCTGGAGAAATAGATGTTTGTTCAAGCCAGCCA
TCCTGCCCTCCTGGTTCTTTTCTTACTCCATTCTTGCAAGCTGTCTACCT
CTTCGTGCAATATATCATCATGGTGAACCTGTTGATTGCTTTCTTCAACA
ACGTTTACTTAGATATGGAATCCATTTCAAATAACCTGTGGAAATACAAC
CGCTATCGCTACATCATGACCTACCACGAGAAGCCCTGGCTGCCCCCACC
TCTCATCCTGCTGAGCCACGTGGGCCTTCTCCTCCGCCGCCTGTGCTGTC
ATCGAGCTCCTCACGACCAAGAAGAGGGTGACGTTGGATTAAAACTCTAC
CTCAGTAAGGAGGATCTGAAAAAACTTCATGATTTTGAGGAGCAGTGCGT
GGAAAAATACTTCCATGAGAAGATGGAAGATGTGAATTGTAGTTGTGAGG
AACGAATCCGAGTGACATCAGAAAGGGTTACAGAGATGTACTTCCAGCTG
AAAGAAATGAATGAAAAGGTGTCTTTTATAAAGGACTCCTTACTGTCTTT
GGACAGCCAGGTGGGACACCTGCAGGATCTCTCTGCCCTGACTGTGGATA
CCCTGAAAGTCCTTTCTGCTGTTGACACTTTGCAAGAGGATGAGGCTCTC
CTGGCCAAGAGAAAGCATTCTACTTGCAAAAAACTTCCCCACAGCTGGAG
CAATGTCATCTGTGCAGAGGTTCTAGGCAGCATGGAGATCGCTGGAGAGA
AGAAATACCAGTATTATAGCATGCCCTCTTCTTTGCTGAGGAGCCTGGCT
GGAGGCCGGCATCCCCCAAGAGTGCAGAGGGGGGCACTTCTTGAGATTAC
AAACAGTAAAAGAGAGGCTACAAATGTAAGAAATGACCAGGAAAGGCAAG
AAACACAAAGTAGTATAGTGGTTTCTGGGGTGTCTCCTAACAGGCAAGCA
CACTCAAAGTATGGCCAGTTCCTTCTGGTCCCCTCTAATCTAAAGCGAGT
TCCTTTTTCAGCAGAAACTGTCTTGCCTCTGTCCAGACCCTCTGTGCCAG
ATGTGCTGGCAACTGAACAGGACATCCAGACTGAGGTTCTTGTTCATCTG
ACTGGGCAGACCCCAGTTGTCTCTGACTGGGCATCAGTGGATGAACCCAA
GGAAAAGCACGAGCCTATTGCTCACTTACTGGATGGACAAGACAAGGCAG
AGCAAGTGCTACCCACTTTGAGTTGCACACCTGAACCCATGACAATGAGC
TCCCCTCTTTCCCAAGCCAAGATCATGCAAACTGGAGGTGGATATGTAAA
CTGGGCATTTTCAGAAGGTGATGAAACTGGTGTGTTTAGCATCAAGAAAA
AGTGGCAAACCTGCTTGCCCTCCACTTGTGACAGTGATTCCTCTCGGAGT
GAACAGCACCAGAAGCAGGCCCAGGACAGCTCCCTATCTGATAACTCAAC
AAGATCGGCCCAGAGTAGTGAATGCTCAGAGGTGGGACCATGGCTTCAGC
CAAACACATCCTTTTGGATCAATCCTCTCCGCAGATACAGGCCCTTCGCT
AGGAGTCATAGTTTTAGATTCCATAAGGAGGAGAAATTGATGAAGATCTG
TAAGATTAAAAATCTTTCAGGCTCTTCAGAAATAGGGCAGGGAGCATGGG
TCAAAGCGAAAATGCTAACCAAAGACAGGAGACTGTCAAAGAAAAAGAAG
AATACTCAAGGACTCCAGGTGCCAATCATAACAGTCAATGCCTGCTCTCA
GAGTGACCAGTTGAATCCAGAGCCAGGAGAAAACAGCATCTCTGAAGAGG
AGTACAGCAAGAACTGGTTCACAGTGTCCAAATTTAGTCACACAGGTGTA
GAACCTTACATACATCAGAAAATGAAAACTAAAGAAATTGGACAATGTGC
TATACAAATCAGTGATTACCTAAAGCAGTCTCAAGAGGATCTCAGCAAAA
ACTCTTTGTGGAATTCCAGGAGCACCAACCTCAATAGGAACTCCCTGCTG
AAAAGTTCAATTGGAGTTGACAAGATCTCAGCCTCCTTAAAAAGCCCTCA
AGAGCCTCACCATCATTATTCAGCCATTGAAAGGAATAATTTAATGAGGC
TTTCTCAGGAGATACCATTTACACCAGTCCAACTGTTTGCAGGAGAAGAA
ATAACCGTCTACAGGTTGGAGGAGAGTTCCCCTTTAAACCTTGATAAAAG
CATGTCCTCTTGGTCTCAGCGTGGGAGAGCGGCAATGATCCAGGTATTGT
CCCGAGAGGAGATGGATGGGGGCCTCCGTAAAGCTATGAGAGTCGTCAGC
ACTTGGTCTGAGGATGACATTCTCAAGCCGGGACAAGTTTTCATTGTCAA
GTCCTTTCTTCCTGAGGTTGTGCGGACATGGCATAAAATCTTCCAGGAGA
GCACTGTGCTTCATCTTTGCCTCAGGGAAATTCAACAACAAAGGGCTGCT
CAAAAATTGATCTATACCTTCAACCAAGTGAAACCACAAACCATACCCTA
CACACCAAGGTTCCTGGAAGTTTTCTTAATCTACTGCCATTCAGCCAACC
AGTGGTTGACCATTGAGAAGTATATGACAGGGGAGTTCCGGAAGTATAAC
AACAACAATGGTGATGAAATCACCCCCACCAACACCCTGGAGGAGCTGAT
GTTGGCTTTCTCTCACTGGACCTATGAGTACACTCGGGGAGAGCTGCTGG
TTTTAGATTTGCAAGGTGTTGGAGAAAATTTGACAGATCCATCTGTTATA
AAACCTGAAGTCAAACAATCAAGAGGAATGGTGTTTGGACCGGCCAATTT
GGGGGAAGATGCAATTAGAAACTTCATTGCAAAACATCATTGTAACTCCT
GCTGCCGGAAGCTCAAACTCCCGGATTTAAAAAGAAATGACTATTCCCCT
GAAAGGATAAATTCCACCTTTGGACTTGAGATAAAAATAGAATCAGCTGA
GGAGCCTCCAGCAAGGGAGACGGGTAGAAATTCCCCAGAAGATGATATGC
AACTATAAAAGGGAATTCGAATTCTGCAGATCGAGAATTCCGCCCCCCCC
CCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGT
TTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGG
GCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTC
CCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAG
TTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGC
AGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCC
ACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTG
TGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCTCCTAAGCGTATTCA
ACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGAT
CTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAA
ACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACAC
GATGATAATATGGCCACAACCATGAGCAAGGGCGAGGAACTGTTCACTGG
CGTGGTCCCAATTCTCGTGGAACTGGATGGCGATGTGAATGGGCACAAAT
TTTCTGTCAGCGGAGAGGGTGAAGGTGATGCCACATACGGAAAGCTCACC
CTGAAATTCATCTGCACCACTGGAAAGCTCCCTGTGCCATGGCCAACACT
GGTCACTACGTTAACCTATGGCGTGCAGTGCTTTTCCAGATACCCAGACC
ATATGAAGCAGCATGACTTTTTCAAGAGCGCCATGCCCGAGGGCTATGTG
CAGGAGAGAACCATCTTTTTCAAAGATGACGGGAACTACAAGACCCGCGC
TGAAGTCAAGTTCGAAGGTGACACCCTGGTGAATAGAATCGAGCTGAAGG
GCATTGACTTTAAGGAGGATGGAAACATTCTCGGCCACAAGCTGGAATAC
AACTATAACTCCCACAATGTGTACATCATGGCCGACAAGCAAAAGAATGG
CATCAAGGTCAACTTCAAGATCAGACACAACATTGAGGATGGATCCGTGC
AGCTGGCCGACCATTATCAACAGAACACTCCAATCGGCGACGGCCCTGTG
CTCCTCCCAGACAACCATTACCTGTCCACCCAGTCTGCCCTGTCTAAAGA
TCCCAACGAAAAGAGAGACCACATGGTCCTGCTGGAGTTTGTGACCGCTG
CTGGGATCACACATGGCATGGACGAGCTGTACAAGTGAGTCGACCCGGGC
GGCCGC

Amino Acid Sequence

MYPYDVPDYAKEQPVLERLQSQKSWIKGVFDKRECSTIIPSSKNPHRCTP
VCQVCQNLIRCYCGRLIGDHAGIDYSWTISAAKGKESEQWSVEKHTTKSP
TDTFGTINFQDGEHTHHAKYIRTSYDTKLDHLLHLMLKEWKMELPKLVIS
VHGGIQNFTMPSKFKEIFSQGLVKAAETTGAWIITEGINTGVSKHVGDAL
KSHSSHSLRKIWTVGIPPWGVIENQRDLIGKDVVCLYQTLDNPLSKLTTL
NSMHSHFILSDDGTVGKYGNEMKLRRNLEKYLSLQKIHCRSRQGVPVVGL
VVEGGPNVILSVWETVKDKDPVVVCEGTGRAADLLAFTHKHLADEGMLRP
QVKEEIICMIQNTFNFSLKQSKHLFQILMECMVHRDCITIFDADSEEQQD
LDLAILTALLKGTNLSASEQLNLAMAWDRVDIAKKHILIYEQHWKPDALE
QAMSDALVMDRVDFVKLLIEYGVNLHRFLTIPRLEELYNTKQGPTNTLLH
HLVQDVKQHTLLSGYRITLIDIGLVVEYLIGRAYRSNYTRKHFRALYNNL
YRKYKHQRHSSGNRNESAESTLHSQFIRTAQPYKFKEKSIVLHKSRKKSK
EQNVSDDPESTGFLYPYNDLLVWAVLMKRQKMAMFFWQHGEEATVKAVIA
CILYRAMAHEAKESHMVDDASEELKNYSKQFGQLALDLLEKAFKQNERMA
MTLLTYELRNWSNSTCLKLAVSGGLRPFVSHTCTQMLLTDMWMGRLKMRK
NSWLKIIISIILPPTILTLEFKSKAEMSHVPQSQDFQFMWYYSDQNASSS
KESASVKEYDLERGHDEKLDENQHFGLESGHQHLPWTRKVYEFYSAPIVK
FWFYTMAYLAFLMLFTYTVLVEMQPQPSVQEWLVSIYIFTNAIEVVREIC
ISEPGKFTQKVKVWISEYWNLTETVAIGLFSAGFVLRWGDPPFHTAGRLI
YCIDIIFWFSRLLDFFAVNQHAGPYVTMIAKMTANMFYIVIIMAIVLLSF
GVARKAILSPKEPPSWSLARDIVFEPYWMIYGEVYAGEIDVCSSQPSCPP
GSFLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLDMESISNNLWKYNRYRY
IMTYHEKPWLPPPLILLSHVGLLLRRLCCHRAPHDQEEGDVGLKLYLSKE
DLKKLHDFEEQCVEKYFHEKMEDVNCSCEERIRVTSERVTEMYFQLKEMN
EKVSFIKDSLLSLDSQVGHLQDLSALTVDTLKVLSAVDTLQEDEALLAKR
KHSTCKKLPHSWSNVICAEVLGSMEIAGEKKYQYYSMPSSLLRSLAGGRH
PPRVQRGALLEITNSKREATNVRNDQERQETQSSIVVSGVSPNRQAHSKY
GQFLLVPSNLKRVPFSAETVLPLSRPSVPDVLATEQDIQTEVLVHLTGQT
PVVSDWASVDEPKEKHEPIAHLLDGQDKAEQVLPTLSCTPEPMTMSSPLS
QAKIMQTGGGYVNWAFSEGDETGVFSIKKKWQTCLPSTCDSDSSRSEQHQ
KQAQDSSLSDNSTRSAQSSECSEVGPWLQPNTSFWINPLRRYRPFARSHS
FRFHKEEKLMKICKIKNLSGSSEIGQGAWVKAKMLTKDRRLSKKKKNTQG
LQVPIITVNACSQSDQLNPEPGENSISEEEYSKNWFTVSKFSHTGVEPYI
HQKMKTKEIGQCAIQISDYLKQSQEDLSKNSLWNSRSTNLNRNSLLKSSI
GVDKISASLKSPQEPHHHYSAIERNNLMRLSQEIPFTPVQLFAGEEITVY
RLEESSPLNLDKSMSSWSQRGRAAMIQVLSREEMDGGLRKAMRVVSTWSE
DDILKPGQVFIVKSFLPEVVRTWHKIFQESTVLHLCLREIQQQRAAQKLI
YTFNQVKPQTIPYTPRFLEVFLIYCHSANQWLTIEKYMTGEFRKYNNNNG
DEITPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEV
KQSRGMVFGPANLGEDAIRNFIAKHHCNSCCRKLKLPDLKRNDYSPERIN
STFGLEIKIESAEEPPARETGRNSPEDDMQL*
.......IRES........MATTMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44506
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: T1724E; ; All TRPM6 plasmids MUST be grown at 30 degrees C or less to prevent recombination.