cDNA Clone TRAK2

• Synonyms: ALS2CR3, CALS-C, GRIF-1, GRIF1, MILT2, OIP98
• Plasmid: pcDNA5frtTO FLAG TRAK2
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG-TRAK2
• Genbank: NM_015049.2
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGAGTCAATCCCAGAATGCAATTTTTACATCACCAACAGGTGAAGAAAACCTCATGAATAGCAATCACAGAGACTCGGAGAGCATCACTGATGTCTGCTCCAATGAGGATCTCCCTGAAGTTGAGCTGGTGAGTCTGCTAGAAGAACAACTACCACAGTATAGGCTAAAAGTAGACACTCTCTTTCTATATGAAAATCAAGACTGGACTCAGTCTCCACACCAGCGGCAGCATGCATCTGATGCTCTCTCTCCAGTCCTTGCTGAAGAGACTTTCCGTTACATGATTCTAGGCACAGACAGGGTGGAGCAGATGACCAAAACTTACAATGACATCGACATGGTTACACATCTCCTGGCAGAGAGGGATCGTGATCTGGAACTCGCTGCTCGAATTGGACAAGCTCTCTTAAAGCGGAACCATATCTTATCTGAGCAGAACGAATCCCTGGAGGAGCAATTGGGACAAGCCTTTGATCAAGTTAATCAGCTGCAGCATGAGCTATGCAAGAAAGATGAGTTACTTCGAATCGTCTCCATTGCTTCTGAAGAAAGTGAAACTGATTCCAGCTGTTCTACACCTCTTCGGTTCAATGAGTCCTTTAGCTTATCTCAAGGGTTGCTGCAGTTGGAAATGCTGCAAGAAAAGCTCAAGGAACTGGAAGAAGAGAATATGGCTCTTCGATCCAAGGCTTGTCACATAAAGACAGAAACTGTTACCTATGAAGAAAAGGAACAACAGCTTGTCAGCGACTGTGTTAAAGAACTTCGTGAAACAAATGCTCAGATGTCCAGAATGACTGAAGAATTGTCAGGGAAGAGTGATGAGCTGGTTCGATACCAAGAAGAGCTTTCCTCTCTTTTGTCACAGATTGTAGACCTTCAGCATAAACTTAAAGAACATGTGATTGAGAAGGAAGAACTAAAACTTCACCTGCAAGCTTCCAAAGATGCCCAACGGCAACTGACAATGGAGCTGCACGAGTTACAAGACAGGAATATGGAGTGTCTAGGAATGTTACATGAATCCCAAGAAGAAATAAAGGAACTTCGTAGTAGATCTGGCCCTACTGCTCATCTCTACTTCTCCCAATCATATGGAGCTTTTACTGGGGAATCTTTGGCAGCTGAGATTGAGGGGACTATGCGTAAAAAGCTGAGTTTGGATGAGGAATCTTCTCTCTTTAAACAAAAAGCCCAACAGAAGCGGGTATTTGATACCGTCAGGATTGCCAATGACACACGGGGCCGCTCTATCTCATTCCCAGCTCTGTTACCCATTCCAGGCTCCAACCGTTCAAGTGTCATCATGACAGCAAAACCTTTTGAGTCTGGTCTTCAGCAAACAGAGGACAAATCACTCCTGAACCAGGGGAGCAGCTCAGAGGAGGTTGCAGGGAGCTCCCAGAAGATGGGCCAACCAGGACCCTCAGGAGATAGTGATTTGGCTACAGCACTGCATCGCCTTAGCTTGCGTCGACAAAACTATTTAAGTGAGAAGCAGTTCTTTGCTGAAGAATGGCAGCGGAAGATCCAGGTTCTGGCAGACCAGAAGGAAGGAGTTAGTGGCTGTGTCACCCCGACAGAGAGCCTTGCCTCTCTCTGCACCACCCAGTCAGAGATCACAGACCTCAGCAGTGCCAGTTGCCTTCGAGGTTTTATGCCAGAAAAATTACAAATTGTCAAGCCCCTTGAAGGATCACAAACTCTGTATCACTGGCAGCAGCTTGCTCAACCAAACTTGGGAACCATCCTTGATCCACGACCAGGTGTCATTACTAAAGGCTTTACCCAGTTGCCCGGGGATGCTATTTATCACATCTCAGATTTAGAAGAGGATGAAGAGGAGGGTATTACTTTTCAGGTTCAGCAACCTCTTGAAGTGGAAGAGAAACTTTCAACATCCAAGCCAGTAACAGGGATCTTCCTGCCACCCATTACTTCAGCAGGTGGACCAGTTACAGTTGCAACCGCCAACCCAGGAAAGTGCCTGTCGTGCACAAACTCAACATTCACTTTCACCACCTGTAGAATATTACATCCCTCTGACATCACTCAGGTTACCCCCAGCTCTGGGTTCCCTTCATTATCCTGTGGAAGTAGCGGTAGCAGTTCATCCAACACGGCTGTGAATTCTCCTGCCTTGTCCTATAGACTCAGCATTGGTGAGTCCATCACCAACCGACGAGATTCCACTACAACCTTCAGTAGCACCATGAGCTTGGCCAAACTTCTACAAGAGCGAGGCATCTCTGCCAAAGTGTACCACAGCCCAATTTCAGAGAACCCCCTCCAGCCTCTCCCTAAATCCCTGGCTATCCCTTCCACACCACCAAATTCACCATCTCACTCACCTTGCCCTTCTCCTTTACCCTTTGAGCCTCGAGTGCATCTCTCTGAAAATTTTTTGGCCTCTCGACCAGCTGAGACATTCCTCCAGGAGATGTATGGCTTGAGACCCTCCCGGAACCCTCCTGATGTTGGCCAGTTGAAGATGAACTTAGTGGACAGGCTGAAGAGACTGGGGATAGCCAGAGTGGTCAAGAACCCTGGTGCCCAAGAGAATGGAAGATGCCAGGAGGCAGAAATTGGTCCTCAAAAACCAGATTCTGCTGTTTATTTAAATTCAGGTAGCAGTTTATTAGGTGGACTAAGGAGGAATCAGAGTCTTCCAGTCATAATGGGTAGCTTTGCTGCCCCAGTTTGCACATCCTCACCCAAAATGGGTGTCCTGAAGGAGGACTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMSQSQNAIFTSPTGEENLMNSNHRDSESITDVCSNEDLP
EVELVSLLEEQLPQYRLKVDTLFLYENQDWTQSPHQRQHASDALSPVLAE
ETFRYMILGTDRVEQMTKTYNDIDMVTHLLAERDRDLELAARIGQALLKR
NHILSEQNESLEEQLGQAFDQVNQLQHELCKKDELLRIVSIASEESETDS
SCSTPLRFNESFSLSQGLLQLEMLQEKLKELEEENMALRSKACHIKTETV
TYEEKEQQLVSDCVKELRETNAQMSRMTEELSGKSDELVRYQEELSSLLS
QIVDLQHKLKEHVIEKEELKLHLQASKDAQRQLTMELHELQDRNMECLGM
LHESQEEIKELRSRSGPTAHLYFSQSYGAFTGESLAAEIEGTMRKKLSLD
EESSLFKQKAQQKRVFDTVRIANDTRGRSISFPALLPIPGSNRSSVIMTA
KPFESGLQQTEDKSLLNQGSSSEEVAGSSQKMGQPGPSGDSDLATALHRL
SLRRQNYLSEKQFFAEEWQRKIQVLADQKEGVSGCVTPTESLASLCTTQS
EITDLSSASCLRGFMPEKLQIVKPLEGSQTLYHWQQLAQPNLGTILDPRP
GVITKGFTQLPGDAIYHISDLEEDEEEGITFQVQQPLEVEEKLSTSKPVT
GIFLPPITSAGGPVTVATANPGKCLSCTNSTFTFTTCRILHPSDITQVTP
SSGFPSLSCGSSGSSSSNTAVNSPALSYRLSIGESITNRRDSTTTFSSTM
SLAKLLQERGISAKVYHSPISENPLQPLPKSLAIPSTPPNSPSHSPCPSP
LPFEPRVHLSENFLASRPAETFLQEMYGLRPSRNPPDVGQLKMNLVDRLK
RLGIARVVKNPGAQENGRCQEAEIGPQKPDSAVYLNSGSSLLGGLRRNQS
LPVIMGSFAAPVCTSSPKMGVLKED*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44541
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Two known residue variations V142I & I278V are present which are benign according to ensembl and have been left in situ.