cDNA Clone UBE2S

• Synonyms: E2-EPF; E2EPF; EPF5
• Plasmid: pGEX6P UBE2S 1-192 -TEV-Ubiquitin 1-75-HA
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-UBE2S 1-192 -TEV-Ubiquitin 1-75-HA
• Genbank: NM_014501.2 GI:112382376 with silent changes c249t, g279a, g435a
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGAACTCCAACGTGGAGAACCTACCCCCGCACATCATCCGCCTGGTGTACAAGGAGGTGACGACACTGACCGCAGACCCACCCGATGGCATCAAGGTCTTTCCCAACGAGGAGGACCTCACCGACCTCCAGGTCACCATCGAGGGCCCTGAGGGGACCCCATATGCTGGAGGTCTGTTCCGCATGAAACTCCTGCTGGGGAAGGACTTCCCTGCCTCCCCACCCAAGGGCTACTTCCTGACCAAGATTTTCCACCCGAACGTGGGCGCCAATGGCGAAATCTGCGTCAACGTGCTCAAGAGGGACTGGACGGCTGAGCTGGGCATCCGACACGTACTGCTGACCATCAAGTGCCTGCTGATCCACCCTAACCCCGAGTCTGCACTCAACGAGGAGGCGGGCCGCCTGCTCTTGGAGAACTACGAGGAGTATGCAGCTCGGGCCCGTCTGCTCACAGAGATCCACGGGGGCGCCGGCGGGCCCAGCGGCAGGGCCGAAGCCGGTCGGGCCCTGGCCAGTGGCACTGAAGCTTCCTCCACCGACCCTGGGGCCCCAGGGGGCCCGGGAGGGGCTGAGGAAAACCTGTATTTTCAGATGCAAATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTCCGTCTCAGAGGTTACCCATACGATGTGCCAGATTACGCCTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNSNVENLPPHIIRLVYKE
VTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKD
FPASPPKGYFLTKIFHPNVGANGEICVNVLKRDWTAELGIRHVLLTIKCL
LIHPNPESALNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGR
ALASGTEASSTDPGAPGGPGGAEENLYFQMQIFVKTLTGKTITLEVEPSD
TIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVL
RLRGYPYDVPDYA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45481
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee