cDNA Clone mTOR

• Plasmid: pFBDual6P-1 mTOR 1362-end
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST-MTor (1362 - end)
• Genbank: NP_004949
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCATGGAACACAGTGACAAGGGCCCCCTGCCACTGAGAGATGACAA
TGGCATTGTTCTGCTGGGTGAGAGAGCTGCCAAGTGCCGAGCATATGCCA
AAGCACTACACTACAAAGAACTGGAGTTCCAGAAAGGCCCCACCCCTGCC
ATTCTAGAATCTCTCATCAGCATTAATAATAAGCTACAGCAGCCGGAGGC
AGCGGCCGGAGTGTTAGAATATGCCATGAAACACTTTGGAGAGCTGGAGA
TCCAGGCTACCTGGTATGAGAAACTGCACGAGTGGGAGGATGCCCTTGTG
GCCTATGACAAGAAAATGGACACCAACAAGGACGACCCAGAGCTGATGCT
GGGCCGCATGCGCTGCCTCGAGGCCTTGGGGGAATGGGGTCAACTCCACC
AGCAGTGCTGTGAAAAGTGGACCCTGGTTAATGATGAGACCCAAGCCAAG
ATGGCCCGGATGGCTGCTGCAGCTGCATGGGGTTTAGGTCAGTGGGACAG
CATGGAAGAATACACCTGTATGATCCCTCGGGACACCCATGATGGGGCAT
TTTATAGAGCTGTGCTGGCACTGCATCAGGACCTCTTCTCCTTGGCACAA
CAGTGCATTGACAAGGCCAGGGACCTGCTGGATGCTGAATTAACTGCAAT
GGCAGGAGAGAGTTACAGTCGGGCATATGGGGCCATGGTTTCTTGCCACA
TGCTGTCCGAGCTGGAGGAGGTTATCCAGTACAAACTTGTCCCCGAGCGA
CGAGAGATCATCCGCCAGATCTGGTGGGAGAGACTGCAGGGCTGCCAGCG
TATCGTAGAGGACTGGCAGAAAATCCTTATGGTGCGGTCCCTTGTGGTCA
GCCCTCATGAAGACATGAGAACCTGGCTCAAGTATGCAAGCCTGTGCGGC
AAGAGTGGCAGGCTGGCTCTTGCTCATAAAACTTTAGTGTTGCTCCTGGG
AGTTGATCCGTCTCGGCAACTTGACCATCCTCTGCCAACAGTTCACCCTC
AGGTGACCTATGCCTACATGAAAAACATGTGGAAGAGTGCCCGCAAGATC
GATGCCTTCCAGCACATGCAGCATTTTGTCCAGACCATGCAGCAACAGGC
CCAGCATGCCATCGCTACTGAGGACCAGCAGCATAAGCAGGAACTGCACA
AGCTCATGGCCCGATGCTTCCTGAAACTTGGAGAGTGGCAGCTGAATCTA
CAGGGCATCAATGAGAGCACAATCCCCAAAGTGCTGCAGTACTACAGCGC
CGCCACAGAGCACGACCGCAGCTGGTACAAGGCCTGGCATGCGTGGGCAG
TGATGAACTTCGAAGCTGTGCTACACTACAAACATCAGAACCAAGCCCGC
GATGAGAAGAAGAAACTGCGTCATGCCAGCGGGGCCAACATCACCAACGC
CACCACTGCCGCCACCACGGCCGCCACTGCCACCACCACTGCCAGCACCG
AGGGCAGCAACAGTGAGAGCGAGGCCGAGAGCACCGAGAACAGCCCCACC
CCATCGCCGCTGCAGAAGAAGGTCACTGAGGATCTGTCCAAAACCCTCCT
GATGTACACGGTGCCTGCCGTCCAGGGCTTCTTCCGTTCCATCTCCTTGT
CACGAGGCAACAACCTCCAGGATACACTCAGAGTTCTCACCTTATGGTTT
GATTATGGTCACTGGCCAGATGTCAATGAGGCCTTAGTGGAGGGGGTGAA
AGCCATCCAGATTGATACCTGGCTACAGGTTATACCTCAGCTCATTGCAA
GAATTGATACGCCCAGACCCTTGGTGGGACGTCTCATTCACCAGCTTCTC
ACAGACATTGGTCGGTACCACCCCCAGGCCCTCATCTACCCACTGACAGT
GGCTTCTAAGTCTACCACGACAGCCCGGCACAATGCAGCCAACAAGATTC
TGAAGAACATGTGTGAGCACAGCAACACCCTGGTCCAGCAGGCCATGATG
GTGAGCGAGGAGCTGATCCGAGTGGCCATCCTCTGGCATGAGATGTGGCA
TGAAGGCCTGGAAGAGGCATCTCGTTTGTACTTTGGGGAAAGGAACGTGA
AAGGCATGTTTGAGGTGCTGGAGCCCTTGCATGCTATGATGGAACGGGGC
CCCCAGACTCTGAAGGAAACATCCTTTAATCAGGCCTATGGTCGAGATTT
AATGGAGGCCCAAGAGTGGTGCAGGAAGTACATGAAATCAGGGAATGTCA
AGGACCTCACCCAAGCCTGGGACCTCTATTATCATGTGTTCCGACGAATC
TCAAAGCAGCTGCCTCAGCTCACATCCTTAGAGCTGCAATATGTTTCCCC
AAAACTTCTGATGTGCCGGGACCTTGAATTGGCTGTGCCAGGAACATATG
ACCCCAACCAGCCAATCATTCGCATTCAGTCCATAGCACCGTCTTTGCAA
GTCATCACATCCAAGCAGAGGCCCCGGAAATTGACACTTATGGGCAGCAA
CGGACATGAGTTTGTTTTCCTTCTAAAAGGCCATGAAGATCTGCGCCAGG
ATGAGCGTGTGATGCAGCTCTTCGGCCTGGTTAACACCCTTCTGGCCAAT
GACCCAACATCTCTTCGGAAAAACCTCAGCATCCAGAGATACGCTGTCAT
CCCTTTATCGACCAACTCGGGCCTCATTGGCTGGGTTCCCCACTGTGACA
CACTGCACGCCCTCATCCGGGACTACAGGGAGAAGAAGAAGATCCTTCTC
AACATCGAGCATCGCATCATGTTGCGGATGGCTCCGGACTATGACCACTT
GACTCTGATGCAGAAGGTGGAGGTGTTTGAGCATGCCGTCAATAATACAG
CTGGGGACGACCTGGCCAAGCTGCTGTGGCTGAAAAGCCCCAGCTCCGAG
GTGTGGTTTGACCGAAGAACCAATTATACCCGTTCTTTAGCGGTCATGTC
AATGGTTGGGTATATTTTAGGCCTGGGAGATAGACACCCATCCAACCTGA
TGCTGGACCGTCTGAGTGGGAAGATCCTGCACATTGACTTTGGGGACTGC
TTTGAGGTTGCTATGACCCGAGAGAAGTTTCCAGAGAAGATTCCATTTAG
ACTAACAAGAATGTTGACCAATGCTATGGAGGTTACAGGCCTGGATGGCA
ACTACAGAATCACATGCCACACAGTGATGGAGGTGCTGCGAGAGCACAAG
GACAGTGTCATGGCCGTGCTGGAAGCCTTTGTCTATGACCCCTTGCTGAA
CTGGAGGCTGATGGACACAAATACCAAAGGCAACAAGCGATCCCGAACGA
GGACGGATTCCTACTCTGCTGGCCAGTCAGTCGAAATTTTGGACGGTGTG
GAACTTGGAGAGCCAGCCCATAAGAAAACGGGGACCACAGTGCCAGAATC
TATTCATTCTTTCATTGGAGACGGTTTGGTGAAACCAGAGGCCCTAAATA
AGAAAGCTATCCAGATTATTAACAGGGTTCGAGATAAGCTCACTGGTCGG
GACTTCTCTCATGATGACACTTTGGATGTTCCAACGCAAGTTGAGCTGCT
CATCAAACAAGCGACATCCCATGAAAACCTCTGCCAGTGCTATATTGGCT
GGTGCCCTTTCTGGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMEHSDKGPLPLRDDNG
IVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAA
AGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLG
RMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSM
EEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMA
GESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRI
VEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGV
DPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQ
HAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAA
TEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITNAT
TAATTAATATTTASTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLM
YTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKA
IQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVA
SKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHE
GLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLM
EAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPK
LLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNG
HEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIP
LSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLT
LMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSM
VGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRL
TRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNW
RLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESI
HSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLI
KQATSHENLCQCYIGWCPFW*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU43771
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee