cDNA Clone UBE2Z

• Synonyms: ubiquitin-conjugating enzyme E2Z (UBE2Z), Use1
• Plasmid: pET156P-1-UBE2Z C100S C154S C243S C254S C261S C263S C286S
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-UBE2Z C100S C154S C243S C254S C261S C263S C286S
• Genbank: NM_023079.3 GI:150417998*
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGGCGGAGAGTCCGACTGAGGAGGCGGCAACGGCGGGCGCCGG
GGCGGCGGGCCCCGGGGCGAGCAGCGTTGCTGGTGTTGTTGGCGTTAGCG
GCAGCGGCGGCGGGTTCGGGCCGCCTTTCCTGCCGGATGTGTGGGCGGCG
GCGGCGGCAGCGGGCGGGGCCGGGGGCCCGGGGAGCGGCCTGGCTCCGCT
GCCCGGGCTCCCGCCCTCAGCCGCTGCCCACGGGGCCGCGCTGCTTAGCC
ACTGGGACCCCACGCTCAGCTCCGACTGGGACGGCGAGCGCACCGCGCCG
CAGTcTCTACTCCGGATCAAGCGGGATATCATGTCCATTTATAAGGAGCC
TCCTCCAGGAATGTTCGTTGTACCTGATACTGTTGACATGACTAAGATTC
ATGCATTGATCACAGGCCCATTTGACACTCCTTATGAAGGGGGTTTCTTC
CTGTTCGTGTTTCGGTcTCCGCCCGACTATCCCATCCACCCACCTCGGGT
CAAACTGATGACAACGGGCAATAACACAGTGAGGTTTAACCCCAACTTCT
ACCGCAATGGGAAAGTCTGCTTGAGTATTCTAGGTACATGGACTGGACCT
GCCTGGAGCCCAGCCCAGAGCATCTCCTCAGTGCTCATCTCTATCCAGTC
CCTGATGACTGAGAACCCCTATCACAATGAGCCCGGCTTTGAACAGGAGA
GACATCCAGGAGACAGCAAAAACTATAATGAATcTATCCGGCACGAGACC
ATCAGAGTTGCAGTCTcTGACATGATGGAAGGAAAGTcTCCCTcTCCTGA
ACCCCTACGAGGGGTGATGGAGAAGTCCTTTCTGGAGTATTACGACTTCT
ATGAGGTGGCCTcCAAAGATCGCCTGCACCTTCAAGGCCAAACTATGCAG
GACCCTTTTGGAGAGAAGCGGGGCCACTTTGACTACCAGTCCCTCTTGAT
GCGCCTGGGACTGATACGTCAGAAAGTGCTGGAGAGGCTCCATAATGAGA
ATGCAGAAATGGACTCTGATAGCAGTTCATCTGGGACAGAGACAGACCTT
CATGGGAGCCTGAGGGTTTAGGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMAESPTEEAATAGAGAAGPGASSVAGV
VGVSGSGGGFGPPFLPDVWAAAAAAGGAGGPGSGLAPLPGLPPSAAAHGA
ALLSHWDPTLSSDWDGERTAPQSLLRIKRDIMSIYKEPPPGMFVVPDTVD
MTKIHALITGPFDTPYEGGFFLFVFRSPPDYPIHPPRVKLMTTGNNTVRF
NPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPG
FEQERHPGDSKNYNESIRHETIRVAVSDMMEGKSPSPEPLRGVMEKSFLE
YYDFYEVASKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLER
LHNENAEMDSDSSSSGTETDLHGSLRV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23976
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_023079.3 CDS: c846t (silent). insert re-subconed from 23921 to fresh backbone following mutagenesis