cDNA Clone EPS8L2

• Synonyms: epidermal growth factor receptor kinase substrate 8-like protein 2 , EPS8-like 2
• Plasmid: pCMV-FLAG-EPS8L2
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-EPS8L2
• Genbank: NP_073609.2 NM_022772.3
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCatgagccagtccggggccgtgagctgctgcccgggtgccaccaa
tggcagcctgggccggtccgacggtgtggccaagatgagccccaaggacc
tgtttgagcagaggaagaagtattccaactccaacgtcatcatgcacgag
acctcgcagtaccacgtccagcacctggccacattcatcatggacaagag
cgaagccatcacgtctgtggacgacgccatccggaagctggtgcagctga
gctccaaggagaagatctggacccaggagatgctgctgcaggtgaacgac
cagtcgttgcggctgctggacatcgagtcacaggaggagctggaagactt
cccgctgcccacggtgcagcgcagccagacggtcctcaaccagctgcgct
acccgtctgtgctgctgctcgtgtgccaggactcggagcagagcaagccg
gatgtccacttcttccactgcgatgaggtggaggcagagctggtgcacga
ggacatcgagagcgcgttggccgactgccggctgggcaagaagatgcggc
cgcagaccctgaagggacaccaggagaagattcggcagcggcagtccatc
ctgcctcctccccagggcccggcgcccatccccttccagcaccgcggcgg
ggattccccggaggccaagaatcgcgtgggcccgcaggtgccactcagcg
agccaggtttccgccgtcgggagtcgcaggaggagccgcgggccgtgctg
gctcagaagatagagaaggagacgcaaattctcaactgcgccctggacga
catcgagtggtttgtggcccggctgcagaaggcagccgaggctttcaagc
agctgaaccagcggaaaaaggggaagaagaagggcaagaaggcgccagca
gagggcgtcctcacactgcgggcacggcccccctctgagggcgagttcat
cgactgcttccagaaaatcaagctggcgattaacttgctggcaaagctgc
agaagcacatccagaaccccagcgccgcggagctcgtgcacttcctcttc
gggcctctggacctgatcgtcaacacctgcagtggcccagacatcgcacg
ctccgtctcctgcccactgctctcccgagatgccgtggacttcctgcgcg
gccacctggtccctaaggagatgtcgctgtgggagtcactgggagagagc
tggatgcggccccgttccgagtggccgcgggagccacaggtgcccctcta
cgtgcccaagttccacagcggctgggagcctcctgtggatgtgctgcagg
aggccccctgggaggtggaggggctggcgtctgcccccatcgaggaggtg
agtccagtgagccgacagtccataagaaactcccagaagcacagccccac
ttcagagcccacccccccgggggatgccctaccaccagtcagctccccac
atactcacaggggctaccagccaacaccagccatggccaagtacgtcaag
atcctgtatgacttcacagcccgaaatgccaacgagctatcggtgctcaa
ggatgaggtcctagaggtgctggaggacggccggcagtggtggaagctgc
gcagccgcagcggccaggcggggtacgtgccctgcaacatcctaggcgag
gcgcgaccggaggacgccggcgccccgttcgagcaggccggtcagaagta
ctggggccccgccagcccgacccacaagctacccccaagcttcccgggga
acaaagacgagctcatgcagcacatggacgaggtcaacgacgagctcatc
cggaaaatcagcaacatcagggcgcagccacagaggcacttccgcgtgga
gcgcagccagcccgtgagccagccgctcacctacgagtcgggtccggacg
aggtccgcgcctggctggaagccaaggccttcagcccgcggatcgtggag
aacctgggcatcctgaccgggccgcagctcttctccctcaacaaggagga
gctgaagaaagtgtgcggcgaggagggcgtccgcgtgtacagccagctca
ccatgcagaaggccttcctggagaagcagcaaagtgggtcggagctggaa
gaactcatgaacaagtttcattccatgaatcagaggaggggggaggacag
ctagGAATTC

Amino Acid Sequence

MDYKDDDDKGSMSQSGAVSCCPGATNGSLGRSDGVAKMSPKDLFEQRKKY
SNSNVIMHETSQYHVQHLATFIMDKSEAITSVDDAIRKLVQLSSKEKIWT
QEMLLQVNDQSLRLLDIESQEELEDFPLPTVQRSQTVLNQLRYPSVLLLV
CQDSEQSKPDVHFFHCDEVEAELVHEDIESALADCRLGKKMRPQTLKGHQ
EKIRQRQSILPPPQGPAPIPFQHRGGDSPEAKNRVGPQVPLSEPGFRRRE
SQEEPRAVLAQKIEKETQILNCALDDIEWFVARLQKAAEAFKQLNQRKKG
KKKGKKAPAEGVLTLRARPPSEGEFIDCFQKIKLAINLLAKLQKHIQNPS
AAELVHFLFGPLDLIVNTCSGPDIARSVSCPLLSRDAVDFLRGHLVPKEM
SLWESLGESWMRPRSEWPREPQVPLYVPKFHSGWEPPVDVLQEAPWEVEG
LASAPIEEVSPVSRQSIRNSQKHSPTSEPTPPGDALPPVSSPHTHRGYQP
TPAMAKYVKILYDFTARNANELSVLKDEVLEVLEDGRQWWKLRSRSGQAG
YVPCNILGEARPEDAGAPFEQAGQKYWGPASPTHKLPPSFPGNKDELMQH
MDEVNDELIRKISNIRAQPQRHFRVERSQPVSQPLTYESGPDEVRAWLEA
KAFSPRIVENLGILTGPQLFSLNKEELKKVCGEEGVRVYSQLTMQKAFLE
KQQSGSELEELMNKFHSMNQRRGEDS*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU23948
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: clone has silent changes at Leu101 (ctg to ttg) and Ile258 (atc to att) compared to NCBI ref