cDNA Clone ARHGEF6

• Synonyms: alpha-PIX; alphaPIX; Cool-2; COOL2; MRX46; PIXA"
• Plasmid: pCMV5.HA ARHGEF6
• Parent Plasmid: pCMV5 HA1
• Expressed: HA ARHGEF6
• Genbank: NM_004840.2 GI:47078220 with g174c (silent to kill BamH1)
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGAATCCAGAAGAACAAATCGTGACATGGCTTATATCTTTGGG
AGTTTTAGAGTCCCCTAAAAAGACCATCTGTGATCCGGAGGAGTTTTTAA
AGTCCTCGCTGAAAAATGGGGTAGTTCTGTGCAAACTGATCAACAGACTC
ATGCCTGGCTCTGTGGAAAAGTTTTGTCTcGATCCCCAAACTGAAGCTGA
CTGCATCAACAACATCAATGACTTCCTGAAAGGATGTGCAACCCTCCAAG
TGGAAATATTTGATCCTGATGACCTTTATTCAGGGGTCAATTTCTCCAAG
GTACTGAGTACTCTTTTAGCTGTCAACAAAGCAACAGAAGATCAGCTATC
AGAAAGACCATGTGGACGTTCCTCTTCTCTTAGTGCTGCTAATACTTCTC
AGACAAACCCACAGGGAGCAGTTTCTAGCACAGTTTCAGGGCTGCAAAGG
CAGTCAAAGACAGTGGAGATGACGGAAAATGGAAGTCATCAGTTGATAGT
AAAAGCAAGATTCAACTTTAAGCAGACTAATGAGGATGAACTGTCAGTTT
GTAAGGGGGACATCATTTACGTCACACGAGTTGAAGAAGGAGGCTGGTGG
GAAGGCACATTAAATGGGAGAACAGGCTGGTTCCCCAGTAATTATGTCCG
TGAAATTAAATCCAGTGAGAGACCTCTCTCCCCAAAAGCCGTCAAAGGAT
TTGAAACTGCTCCACTTACCAAGAATTATTATACTGTGGTGTTACAGAAC
ATCCTGGACACTGAAAAAGAATATGCTAAAGAACTTCAGTCTCTTCTTGT
TACTTACTTAAGACCCCTGCAGTCCAATAACAATCTGAGTACTGTGGAGG
TTACATCTTTACTGGGAAACTTCGAGGAAGTATGCACATTTCAACAGACA
CTCTGCCAAGCCTTGGAAGAATGTTCAAAGTTTCCAGAAAACCAGCACAA
AGTAGGAGGTTGTCTACTGAGTCTCATGCCTCATTTTAAATCTATGTATC
TGGCTTACTGTGCAAACCATCCTTCAGCTGTAAATGTGCTCACTCAGCAC
AGTGATGAGTTGGAACAATTCATGGAAAATCAAGGTGCATCGAGCCCAGG
TATCCTCATTTTAACAACAAACCTCAGCAAACCATTCATGCGACTGGAGA
AATATGTTACTCTCTTGCAAGAGTTAGAACGGCATATGGAGGATACTCAT
CCAGATCATCAGGATATTCTGAAAGCAATCGTAGCATTCAAAACTCTCAT
GGGGCAATGTCAAGATCTGAGGAAGAGAAAACAGCTGGAGTTACAGATAC
TGTCCGAACCTATTCAGGCATGGGAAGGAGAAGATATTAAAAACTTGGGA
AATGTGATTTTTATGTCACAAGTAATGGTGCAGTATGGAGCATGTGAGGA
AAAAGAGGAGCGGTACCTTATGTTATTTTCAAATGTCCTGATAATGTTAT
CTGCAAGTCCTCGGATGAGTGGCTTTATCTATCAGGGAAAAATACCAATA
GCAGGAACGGTGGTGACTAGATTAGATGAAATTGAAGGGAATGACTGCAC
ATTTGAAATCACTGGTAACACAGTGGAGAGAATTGTGGTCCATTGTAACA
ACAACCAGGACTTCCAGGAATGGTTGGAGCAGCTGAACAGACTGATCAGA
GGACCTGCCTCTTGCAGTTCATTATCCAAAACCTCATCGTCATCATGTAG
TGCTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTGGAGC
CTCCTCAAATTATAAAACCGTGGAGTTTAAGTTGTCTACGACCTGCACCT
CCACTTAGACCATCAGCAGCACTAGGTTATAAAGAGAGGATGTCTTATAT
CTTAAAGGAGTCTAGTAAAAGCCCTAAAACGATGAAGAAATTTCTTCATA
AAAGGAAGACTGAGAGAAAACCATCGGAGGAGGAATATGTGATTAGGAAA
AGTACAGCTGCTCTGGAAGAGGATGCTCAAATCCTTAAAGTGATCGAAGC
CTACTGCACCAGCGCAAATTTTCAACAAGGCCATGGCTCAAGTACTCGAA
AAGATTCCATTCCACAAGTCCTACTCCCTGAGGAAGAGAAACTCATCATT
GAAGAAACCAGAAGCAACGGCCAGACCATCATGGAAGAAAAGAGCCTTGT
TGATACTGTTTACGCCTTGAAGGACGAGGTCAGAGAACTGAAGCAGGAAA
ATAAAAGAATGAAGCAATGCCTGGAAGAAGAACTGAAATCAAGAAGGGAC
CTAGAAAAGCTGGTGCGGAGGCTTTTGAAGCAAACAGATGAGTGTATTCG
AGGCGAGTCCAGTAGCAAGACCTCAATTCTTCCATAAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMNPEEQIVTWLISLGVLESPKKTICDPEEFLKSSLKNG
VVLCKLINRLMPGSVEKFCLDPQTEADCINNINDFLKGCATLQVEIFDPD
DLYSGVNFSKVLSTLLAVNKATEDQLSERPCGRSSSLSAANTSQTNPQGA
VSSTVSGLQRQSKTVEMTENGSHQLIVKARFNFKQTNEDELSVCKGDIIY
VTRVEEGGWWEGTLNGRTGWFPSNYVREIKSSERPLSPKAVKGFETAPLT
KNYYTVVLQNILDTEKEYAKELQSLLVTYLRPLQSNNNLSTVEVTSLLGN
FEEVCTFQQTLCQALEECSKFPENQHKVGGCLLSLMPHFKSMYLAYCANH
PSAVNVLTQHSDELEQFMENQGASSPGILILTTNLSKPFMRLEKYVTLLQ
ELERHMEDTHPDHQDILKAIVAFKTLMGQCQDLRKRKQLELQILSEPIQA
WEGEDIKNLGNVIFMSQVMVQYGACEEKEERYLMLFSNVLIMLSASPRMS
GFIYQGKIPIAGTVVTRLDEIEGNDCTFEITGNTVERIVVHCNNNQDFQE
WLEQLNRLIRGPASCSSLSKTSSSSCSAHSSFSSTGQPRGPLEPPQIIKP
WSLSCLRPAPPLRPSAALGYKERMSYILKESSKSPKTMKKFLHKRKTERK
PSEEEYVIRKSTAALEEDAQILKVIEAYCTSANFQQGHGSSTRKDSIPQV
LLPEEEKLIIEETRSNGQTIMEEKSLVDTVYALKDEVRELKQENKRMKQC
LEEELKSRRDLEKLVRRLLKQTDECIRGESSSKTSILP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45525
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee