cDNA Clone CAND1

• Synonyms: cullin-associated and neddylation-dissociated 1
• Plasmid: pGEX6P-2-Cand1
• Parent Plasmid: pGEX6P2
• Expressed: GST-Cand1
• Genbank: NM_018448.3.
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgccATGGCGAGCGCCTCGTACCACATTTCCAATTTGCTGGAAAAAATGACATCCAGCGACAAGGACTTTAGGTTTATGGCTACAAATGATTTGATGACGGAACTGCAGAAAGATTCCATCAAGTTGGATGATGATAGTGAAAGGAAAGTAGTGAAAATGATTTTGAAGTTATTGGAAGATAAAAATGGAGAGGTACAGAATTTAGCTGTCAAATGTCTTGGTCCTTTAGTGAGTAAAGTGAAAGAATACCAAGTAGAGACAATTGTAGATACCCTCTGCACTAACATGCTTTCTGATAAAGAACAACTTCGAGACATTTCAAGTATTGGTCTTAAAACAGTAATTGGAGAACTTCCTCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGTCTTACAAGTGCAATAGCAAAACAGGAAGATGTCTCTGTTCAGCTAGAAGCCTTGGATATTATGGCTGATATGTTGAGCAGGCAAGGAGGACTTCTTGTTAATTTCCATCCTTCAATTCTGACCTGTCTACTTCCCCAGTTGACCAGCCCTAGACTTGCAGTGAGGAAAAGAACCATTATCGCTCTTGGCCATCTGGTTATGAGCTGTGGAAATATAGTTTTTGTAGATCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGATTCTATGTCAACAACAAGAACCTACATACAATGTATTGCTGCTATTAGTAGGCAAGCTGGTCATAGAATAGGTGAATACCTTGAGAAGATAATTCCTTTGGTGGTAAAATTTTGCAATGTAGATGATGATGAATTAAGAGAGTACTGTATTCAAGCCTTTGAATCATTTGTAAGAAGATGTCCTAAGGAAGTATATCCTCATGTTTCTACCATTATAAATATTTGTCTTAAATATCTTACCTATGATCCAAATTATAATTACGATGATGAAGATGAAGATGAAAATGCAATGGATGCTGATGGTGGTGATGATGATGATCAAGGGAGTGATGATGAATACAGTGATGATGATGACATGAGTTGGAAAGTGAGACGTGCAGCTGCGAAGTGCTTGGATGCTGTAGTTAGCACAAGGCATGAAATGCTTCCAGAATTCTACAAGACCGTCTCTCCTGCACTAATATCCAGATTTAAAGAGCGTGAAGAGAATGTAAAGGCAGATGTTTTTCACGCATACCTTTCTCTTTTGAAGCAAACTCGTCCTGTACAAAGTTGGCTATGTGACCCTGATGCAATGGAGCAGGGAGAAACACCTTTAACAATGCTTCAGAGTCAGGTTCCCAACATTGTTAAAGCTCTTCACAAACAGATGAAAGAAAAAAGTGTGAAGACCCGACAGTGTTGTTTTAACATGTTAACTGAGCTGGTAAATGTATTACCTGGGGCCCTAACTCAACACATTCCTGTACTTGTACCAGGAATCATTTTCTCACTGAATGATAAATCAAGCTCATCGAATTTGAAGATCGATGCTTTGTCATGTCTATACGTAATCCTCTGTAACCATTCTCCTCAAGTCTTCCATCCTCACGTTCAGGCTTTGGTTCCTCCAGTGGTGGCTTGTGTTGGAGACCCATTTTACAAAATTACATCTGAAGCACTTCTTGTTACTCAACAGCTTGTCAAAGTAATTCGTCCTTTAGATCAGCCTTCCTCGTTTGATGCAACTCCTTATATCAAAGATCTATTTACCTGTACCATTAAGAGATTAAAAGCAGCTGACATTGATCAGGAAGTCAAGGAAAGGGCTATTTCCTGTATGGGACAAATTATTTGCAACCTTGGAGACAATTTGGGTTCTGACTTGCCTAATACACTTCAGATTTTCTTGGAGAGACTAAAGAATGAAATTACCAGGTTAACTACAGTAAAGGCATTGACACTGATTGCTGGGTCACCTTTGAAGATAGATTTGAGGCCTGTTCTGGGAGAAGGGGTTCCTATCCTTGCTTCATTTCTTAGAAAAAACCAGAGAGCTTTGAAACTGGGTACTCTTTCTGCCCTTGATATTCTAATAAAAAACTATAGTGACAGCTTGACAGCTGCCATGATTGATGCAGTTCTAGATGAGCTCCCACCTCTTATCAGCGAAAGTGATATGCATGTTTCACAAATGGCCATCAGTTTTCTTACCACTTTGGCAAAAGTATATCCCTCCTCCCTTTCAAAGATAAGTGGATCCATTCTCAATGAACTTATTGGACTTGTGAGATCACCCTTATTGCAGGGGGGAGCTCTTAGTGCCATGCTAGACTTTTTCCAAGCTCTGGTTGTCACTGGAACAAATAATTTAGGATACATGGATTTGTTGCGCATGCTGACTGGTCCAGTTTACTCTCAGAGCACAGCTCTTACTCATAAGCAGTCTTATTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCATGCCCTAAAGAGGGACCAGCTGTAGTAGGTCAGTTTATTCAAGATGTCAAGAACTCAAGGTCTACAGATTCCATTCGTCTCTTAGCTCTACTTTCTCTTGGAGAAGTTGGGCATCATATTGACTTAAGTGGACAGTTGGAACTAAAATCTGTAATACTAGAAGCTTTCTCATCTCCTAGTGAAGAAGTCAAATCAGCTGCATCCTATGCATTAGGCAGCATTAGTGTGGGCAACCTTCCTGAATATCTACCGTTTGTCCTGCAAGAAATAACTAGTCAACCCAAAAGGCAGTATCTTTTACTTCATTCCTTGAAGGAAATTATTAGCTCTGCATCAGTGGTGGGCCTTAAACCATATGTTGAAAACATCTGGGCCTTATTACTAAAGCACTGTGAGTGTGCAGAGGAAGGAACCAGAAATGTTGTTGCTGAATGTCTAGGAAAACTCACTCTAATTGATCCAGAAACTCTCCTTCCACGGCTTAAGGGGTACTTGATATCAGGCTCATCATATGCCCGAAGCTCAGTGGTTACGGCTGTGAAATTTACAATTTCTGACCATCCACAACCTATTGATCCACTGTTAAAGAACTGCATAGGTGATTTCCTAAAAACTTTGGAAGACCCAGATTTGAATGTGAGAAGAGTAGCCTTGGTCACATTTAATTCAGCAGCACATAACAAGCCATCATTAATAAGGGATCTATTGGATACTGTTCTTCCACATCTTTACAATGAAACAAAAGTTAGAAAGGAGCTTATAAGAGAGGTAGAAATGGGTCCATTTAAACATACGGTTGATGATGGTCTGGATATTAGAAAGGCAGCATTTGAGTGTATGTACACACTTCTAGACAGTTGTCTTGATAGACTTGATATCTTTGAATTTCTAAATCATGTTGAAGATGGTTTGAAGGACCATTATGATATTAAGATGCTGACATTTTTAATGTTGGTGAGACTGTCTACCCTTTGTCCAAGTGCAGTACTGCAGAGGTTGGACCGACTTGTTGAGCCATTACGTGCAACATGTACAACTAAGGTAAAGGCAAACTCAGTAAAGCAGGAGTTTGAAAAACAAGATGAATTAAAGCGATCTGCCATGAGAGCAGTAGCAGCACTACTAACCATTCCAGAAGCAGAGAAGAGTCCACTGATGAGTGAATTCCAGTCACAGATCAGTTCTAACCCTGAGCTGGCGGCTATCTTTGAAAGTATCCAGAAAGATTCATCATCTACTAACTTGGAATCAATGGACACTAGTTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGIPGSTRAAAMASASYHI
SNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLE
DKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSI
GLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIM
ADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGN
IVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIP
LVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYD
PNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLD
AVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPV
QSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNML
TELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNH
SPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSS
FDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDL
PNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASF
LRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHV
SQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDF
FQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTR
ACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELK
SVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYL
LLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKL
TLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCI
GDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKV
RKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNH
VEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVK
ANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPEL
AAIFESIQKDSSSTNLESMDTS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24006
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Prescission cleavage