cDNA Clone ITK

• Plasmid: pFBHTb ITK 360-612
• Parent Plasmid: pFastBac HTb
• Expressed: His-ITK (360 - 612)
• Genbank: NM_005546
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCCCCTCAGAGCTCACTTTTGTGCAAGAGATTGGCAGTGGGCAATT TGGGTTGGTGCATCTGGGCTACTGGCTCAACAAGGACAAGGTGGCTATCA AAACCATTCGGGAAGGGGCTATGTCAGAAGAGGACTTCATAGAGGAGGCT GAAGTAATGATGAAACTCTCTCATCCCAAACTGGTGCAGCTGTATGGGGT GTGCCTGGAGCAGGCCCCCATCTGCCTGGTGTTTGAGTTCATGGAGCACG GCTGCCTGTCAGATTATCTACGCACCCAGCGGGGACTTTTTGCTGCAGAG ACCCTGCTGGGCATGTGTCTGGATGTGTGTGAGGGCATGGCCTACCTGGA AGAGGCATGTGTCATCCACAGAGACTTGGCTGCCAGAAATTGTTTGGTGG GAGAAAACCAAGTCATCAAGGTGTCTGACTTTGGGATGACAAGGTTCGTT CTGGATGATCAGTACACCAGTTCCACAGGCACCAAATTCCCGGTGAAGTG GGCATCCCCAGAGGTTTTCTCTTTCAGTCGCTATAGCAGCAAGTCCGATG TGTGGTCATTTGGTGTGCTGATGTGGGAAGTTTTCAGTGAAGGCAAAATC CCGTATGAAAACCGAAGCAACTCAGAGGTGGTGGAAGACATCAGTACCGG ATTTCGGTTGTACAAGCCCCGGCTGGCCTCCACACACGTCTACCAGATTA TGAATCACTGCTGGAAAGAGAGACCAGAAGATCGGCCAGCCTTCTCCAGA CTGCTGCGTCAACTGTAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSPSELTFVQEIGSGQFGLVHLGY
WLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPI
CLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHR
DLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFS
FSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPR
LASTHVYQIMNHCWKERPEDRPAFSRLLRQL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU43898
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Insert sequenced in DU43873 but impossible to sequence beyond the BamH1 in pFBHTb ( reverse sequence OK until GGATCC)