cDNA Clone HOIP

• Synonyms: RNF31; ZIBRA
• Plasmid: pcDNA5D FRT/TO GFP HOIP siRNA resistant
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-HOIP siRNA resistant
• Genbank: NM_017999.4
• Tag: GFP
• Species: Human

Sequence of Insert

gcggccgcgATGCCGGGGGAGGAAGAGGAGCGGGCCTTCCTGGTGGCCCGCGAGGAGCTGGCGAGCGCCCTGAGGAGGGATTCCGGGCAGGCGTTTTCCCTGGAGCAGCTCCGGCCGCTACTAGCCAGCTCTCTGCCGCTAGCCGCCCGCTACCTGCAGCTGGACGCCGCACGCCTTGTCCGCTGCAACGCTCATGGGGAGCCCCGAAACTACCTCAACACCCTGTCCACGGCTCTGAACATCCTGGAGAAATACGGCCGCAACCTTCTCAGCCCTCAGCGGCCTCGGTACTGGCGTGGTGTCAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGGGGGCCGAGATGTGCTGCGATTATATGGCTACACAGAGGAGCAACCAGATGGGTTGAGCTTCCCCGAAGGGCAGGAGGAGCCAGATGAGCACCAGGTTGCTACAGTCACACTGGAAGTACTGCTGCTTCGGACAGAGCTCAGCCTGCTATTGCAGAATACTCATCCAAGACAGCAGGCACTGGAGCAGCTGTTGGAAGACAAGGTTGAAGATGATATGCTGCAGCTTTCAGAATTTGACCCCCTATTGAGAGAGATTGCTCCTGGCCCCCTCACCACACCCTCTGTCCCAGGCTCCACTCCTGGTCCCTGCTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGCCCATCCTGTAAACAGGCCCTGTGTCCAGCCTGTGACCACCTGTTCCATGGACACCCATCCCGTGCTCATCACCTCCGCCAGACCCTGCCTGGGGTCCTGCAGGGTACCCACCTGAGCCCCAGTTTACCTGCCTCAGCCCAACCACGGCCCCAGTCGACCTCCCTGCTGGCCCTGGGAGACAGCTCTCTTTCTTCCCCTAATCCTGCAAGTGCTCATTTGCCCTGGCACTGTGCTGCCTGTGCCATGCTAAATGAGCCTTGGGCAGTGCTCTGTGTGGCCTGTGATCGGCCCCGAGGCTGTAAGGGGTTGGGGTTGGGAACTGAGGGTCCCCAAGGAACTGGAGGCTTAGAGCCGGACTTAGCTCGGGGTCGGTGGGCCTGCCAGAGCTGTACCTTTGAGAATGAGGCAGCTGCTGTGCTATGTTCCATATGTGAGCGACCTCGGCTGGCCCAGCCTCCCAGCTTGGTGGTGGATTCCCGAGATGCTGGCATTTGCCTGCAACCCCTTCAGCAGGGGGATGCTTTGCTGGCCTCTGCCCAGAGTCAAGTCTGGTACTGTATTCACTGTACCTTCTGCAACTCGAGCCCTGGCTGGGTGTGTGTTATGTGCAACCGGACTAGTAGCCCCATTCCAGCACAACATGCCCCCCGGCCCTATGCCAGCTCTTTGGAAAAGGGACCCCCCAAGCCTGGGCCCCCACGACGCCTTAGTGCCCCCCTGCCCAGTTCCTGTGGAGATCCTGAGAAGCAGCGCCAAGACAAGATGCGGGAAGAAGGCCTCCAGCTAGTGAGCATGATCCGGGAAGGGGAAGCCGCAGGTGCCTGTCCAGAGGAGATCTTCTCGGCTCTGCAGTACTCGGGCACTGAGGTGCCTCTGCAGTGGTTGCGCTCAGAACTGCCCTACGTCCTGGAGATGGTGGCTGAGCTGGCTGGACAGCAGGACCCTGGGCTGGGTGCCTTTTCCTGTCAGGAGGCCCGGAGAGCCTGGCTGGATCGTCATGGCAACCTTGATGAAGCTGTGGAGGAGTGTGTGAGGACCAGGCGAAGGAAGGTGCAGGAGCTCCAGTCTCTAGGCTTTGGGCCTGAGGAGGGGTCTCTCCAGGCATTGTTCCAGCACGGAGGTGATGTGTCACGGGCCCTGACTGAGCTACAGCGCCAACGCCTAGAGCCCTTCCGCCAGCGCCTCTGGGACAGTGGCCCTGAGCCCACCCCTTCCTGGGATGGGCCAGACAAGCAGAGCCTGGTCAGGCGGCTTTTGGCAGTCTACGCACTCCCCAGCTGGGGCCGGGCAGAGCTGGCACTGTCACTGCTGCAGGAGACACCCAGGAACTATGAGTTGGGGGATGTGGTAGAAGCTGTGAGGCACAGCCAGGACCGGGCCTTCCTGCGCCGCTTGCTTGCCCAGGAGTGTGCCGTGTGTGGCTGGGCCCTGCCCCACAACCGGATGCAGGCCCTGACTTCCTGTGAGTGCACCATCTGTCCTGACTGCTTCCGCCAGCACTTCACCATCGCCTTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGTGGCCGCCCCGACCTCACCGATGACACACAGTTGCTCAGCTACTTCTCTACCCTTGACATCCAGCTTCGCGAGAGCCTAGAGCCAGATGCCTATGCGTTGTTCCATAAGAAGCTGACCGAGGGTGTGCTGATGCGGGACCCCAAGTTCTTGTGGTGTGCCCAGTGCTCCTTTGGCTTCATATATGAGCGTGAGCAGCTGGAGGCAACTTGTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCCAGTGGGAGGAGCAGCACCGAGGTCGGAGCTGTGAGGACTTCCAGAACTGGAAACGCATGAACGACCCAGAATACCAGGCCCAGGGCCTAGCAATGTATCTTCAGGAAAACGGCATTGACTGCCCCAAATGCAAGTTCTCGTACGCCCTGGCCCGAGGAGGCTGCATGCACTTTCACTGTACCCAGTGCCGCCACCAGTTCTGCAGCGGCTGCTACAATGCCTTTTACGCCAAGAATAAATGTCCAGAGCCTAACTGCAGGGTGAAAAAGTCCCTGCACGGCCACCACCCTCGAGACTGCCTCTTCTACCTGCGGGACTGGACTGCTCTCCGGCTTCAGAAGCTGCTACAGGACAATAACGTCATGTTTAATACAGAGCCTCCAGCTGGGGCCCGGGCAGTCCCTGGAGGCGGCTGCCGAGTGATAGAGCAGAAGGAGGTTCCCAATGGGCTCAGGGACGAAGCTTGTGGCAAGGAAACTCCAGCTGGCTATGCCGGCCTGTGCCAGGCACACTACAAAGAGTATCTTGTGAGCCTCATCAATGCCCACTCGCTGGACCCAGCCACCTTGTATGAGGTGGAAGAGCTGGAGACGGCCACTGAGCGCTACCTGCACGTACGCCCCCAGCCTTTGGCTGGAGAGGATCCCCCTGCTTACCAGGCCCGCTTGTTACAGAAGCTGACAGAAGAGGTACCCTTGGGACAGAGTATCCCCCGCAGGCGGAAGTAGGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSRKGTIDAAAMPGEEEERAFLVAREELASALRRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPSVPGSTPGPCFLCGSAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTLPGVLQGTHLSPSLPASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAACAMLNEPWAVLCVACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQSCTFENEAAAVLCSICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASAQSQVWYCIHCTFCNSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPKPGPPRRLSAPLPSSCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEIFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAWLDRHGNLDEAVEECVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRALTELQRQRLEPFRQRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPHNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25010
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has the following silent mutations for siRNA resistance; ; L345 CTA to TTA; E346 GAA to GAG; P347 CCT to CCG; D348 GAT to GAC; L349 CTT to TTA; A350 GCA to GCT; ; So amino acid sequence is the same; Parental Plasmid DU41455