cDNA Clone Ubr1

• Synonyms: ubiquitin protein ligase E3 component n-recognin 1
• Plasmid: pEFIRES-P-eYFP-C1-Ubr1 (leu 253 Ile)
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eYFP-Ubr1 (leu 253 Ile)
• Genbank: Gene 197131 NM_174916.2
• Tag: YFP
• Species: Human

Sequence of Insert

GTCGACgtATGGCGGACGAGGAGGCTGGAGGTACTGAGAGGATGGAAATC
AGCGCGGAGTTACCCCAGACCCCTCAGCGTCTGGCATCTTGGTGGGATCA
GCAAGTTGATTTTTATACTGCTTTCTTGCATCATTTGGCACAATTGGTGC
CAGAAATTTACTTTGCTGAAATGGACCCAGACTTGGAAAAGCAGGAGGAA
AGTGTACAAATGTCAATATTCACTCCACTGGAATGGTACTTATTTGGAGA
AGATCCAGATATTTGCTTAGAGAAATTGAAGCACAGTGGAGCATTTCAGC
TTTGTGGGAGGGTTTTCAAAAGTGGAGAGACAACCTATTCTTGCAGGGAT
TGTGCAATTGATCCAACATGTGTACTCTGTATGGACTGCTTCCAGGACAG
TGTTCATAAAAATCATCGTTACAAGATGCATACTTCTACTGGAGGAGGGT
TCTGTGACTGTGGAGACACAGAGGCATGGAAAACTGGCCCTTTTTGTGTA
AATCATGAACCTGGAAGAGCAGGTACTATAAAAGAGAATTCACGCTGTCC
GTTGAATGAAGAGGTAATTGTCCAAGCCAGGAAAATATTTCCTTCAGTGA
TAAAATATGTCGTAGAAATGACTATATGGGAAGAGGAAAAAGAACTGCCT
CCTGAACTCCAGATAAGGGAGAAAAATGAAAGATACTATTGTGTCCTTTT
CAATGATGAACACCATTCATATGACCACGTCATATACAGCCTACAAAGAG
CTCTTGACTGTGAGaTCGCAGAGGCCCAGTTGCATACCACTGCCATTGAC
AAAGAGGGTCGTCGGGCTGTTAAAGCGGGAGCTTATGCTGCTTGCCAGGA
AGCAAAGGAAGATATAAAGAGTCATTCAGAAAATGTCTCTCAACATCCAC
TTCATGTAGAAGTATTACACTCAGAGATTATGGCTCATCAGAAATTTGCT
TTGCGTCTTGGTTCCTGGATGAACAAAATTATGAGCTATTCAAGTGACTT
TAGGCAGATCTTTTGCCAAGCATGCCTTAGAGAAGAACCTGACTCGGAGA
ATCCCTGTCTCATAAGCAGGTTAATGCTTTGGGATGCAAAGCTTTATAAA
GGTGCCCGTAAGATCCTTCATGAATTGATCTTCAGCAGTTTTTTTATGGA
GATGGAATACAAAAAACTCTTTGCTATGGAATTTGTGAAGTATTATAAAC
AACTGCAGAAAGAATATATCAGTGATGATCATGACAGAAGTATCTCTATA
ACTGCACTTTCAGTTCAGATGTTTACTGTTCCTACTCTGGCTCGACATCT
TATTGAAGAGCAGAATGTTATCTCTGTCATTACTGAAACTCTGCTAGAAG
TTTTACCTGAGTACTTGGACAGGAACAATAAATTCAACTTCCAGGGTTAT
AGCCAGGACAAATTGGGAAGAGTATATGCAGTAATATGTGACCTAAAGTA
TATCCTGATCAGCAAACCCACAATATGGACAGAAAGATTAAGAATGCAGT
TCCTTGAAGGTTTTCGATCTTTTTTGAAGATTCTTACCTGTATGCAGGGA
ATGGAAGAAATCCGAAGACAGGTTGGGCAACACATTGAAGTGGATCCTGA
TTGGGAGGCTGCCATTGCTATACAGATGCAATTGAAGAATATTTTACTCA
TGTTCCAAGAGTGGTGTGCTTGTGATGAAGAACTCTTACTTGTGGCTTAT
AAAGAATGTCACAAAGCTGTGATGAGGTGCAGTACCAGTTTCATATCTAG
TAGCAAGACAGTAGTACAATCGTGTGGACATAGTTTGGAAACAAAGTCCT
ACAGAGTATCTGAGGATCTTGTAAGCATACATCTGCCACTCTCTAGGACC
CTTGCTGGTCTTCATGTACGTTTAAGCAGGCTGGGTGCTGTTTCAAGACT
GCATGAATTTGTGTCTTTTGAGGACTTTCAAGTAGAGGTACTAGTGGAAT
ATCCTTTACGTTGTCTGGTGTTGGTTGCCCAGGTTGTTGCTGAGATGTGG
CGAAGAAATGGACTGTCTCTTATTAGCCAGGTGTTTTATTACCAAGATGT
TAAGTGCAGAGAAGAAATGTATGATAAAGATATCATCATGCTTCAGATTG
GTGCATCTTTAATGGATCCCAATAAGTTCTTGTTACTGGTACTTCAGAGG
TATGAACTTGCCGAGGCTTTTAACAAGACCATATCTACAAAAGACCAGGA
TTTGATTAAACAATATAATACACTAATAGAAGAAATGCTTCAGGTCCTCA
TCTATATTGTGGGTGAGCGTTATGTACCTGGAGTGGGAAATGTGACCAAA
GAAGAGGTCACAATGAGAGAAATCATTCACTTGCTTTGCATTGAACCCAT
GCCACACAGTGCCATTGCCAAAAATTTACCTGAGAATGAAAATAATGAAA
CTGGCTTAGAGAATGTCATAAACAAAGTGGCCACATTTAAGAAACCAGGT
GTATCAGGCCATGGAGTTTATGAACTAAAAGATGAATCACTGAAAGACTT
CAATATGTACTTTTATCATTACTCCAAAACCCAGCATAGCAAGGCTGAAC
ATATGCAGAAGAAAAGGAGAAAACAAGAAAACAAAGATGAAGCATTGCCG
CCACCACCACCTCCTGAATTCTGCCCTGCTTTCAGCAAAGTGATTAACCT
TCTCAACTGTGATATCATGATGTACATTCTCAGGACCGTATTTGAGCGGG
CAATAGACACAGATTCTAACTTGTGGACCGAAGGGATGCTCCAAATGGCT
TTTCATATTCTGGCATTGGGTTTACTAGAAGAGAAGCAACAGCTTCAAAA
AGCTCCTGAAGAAGAAGTAACATTTGACTTTTATCATAAGGCTTCAAGAT
TGGGAAGTTCAGCCATGAATATACAAATGCTTTTGGAAAAACTCAAAGGA
ATTCCCCAGTTAGAAGGCCAGAAGGACATGATAACGTGGATACTTCAGAT
GTTTGACACAGTGAAGCGATTAAGAGAAAAATCTTGTTTAATTGTAGCAA
CCACATCAGGATCGGAATCTATTAAGAATGATGAGATTACTCATGATAAA
GAAAAAGCAGAACGAAAAAGAAAAGCTGAAGCTGCTAGGCTACATCGCCA
GAAGATCATGGCTCAGATGTCTGCCTTACAGAAAAACTTCATTGAAACTC
ATAAACTCATGTATGACAATACATCAGAAATGCCTGGGAAAGAAGATTCC
ATTATGGAGGAAGAGAGCACCCCAGCAGTCAGTGACTACTCTAGAATTGC
TTTGGGTCCTAAACGGGGTCCATCTGTTACTGAAAAGGAGGTGCTGACGT
GCATCCTTTGCCAAGAAGAACAGGAGGTGAAAATAGAAAATAATGCCATG
GTATTATCGGCCTGTGTCCAGAAATCTACTGCCTTAACCCAGCACAGGGG
AAAACCCATAGAACTCTCAGGAGAAGCCCTAGACCCACTTTTCATGGATC
CAGACTTGGCATATGGAACTTATACAGGAAGCTGTGGTCATGTAATGCAC
GCAGTGTGCTGGCAGAAGTATTTTGAAGCTGTACAGCTGAGCTCTCAGCA
GCGCATTCATGTTGACCTTTTTGACTTGGAAAGTGGAGAATATCTTTGCC
CTCTTTGCAAATCTCTGTGCAATACTGTGATCCCCATTATTCCTTTGCAA
CCTCAAAAGATAAACAGTGAGAATGCAGATGCTCTTGCTCAACTTTTGAC
CCTGGCACGGTGGATACAGACTGTTCTGGCCAGAATATCAGGTTATAATA
TAAGACATGCTAAAGGAGAAAACCCAATTCCTATTTTCTTTAATCAAGGA
ATGGGAGATTCTACTTTGGAGTTCCATTCCATCCTGAGTTTTGGCGTTGA
GTCTTCGATTAAATATTCAAATAGCATCAAGGAAATGGTTATTCTCTTTG
CCACAACAATTTATAGAATTGGATTGAAAGTGCCACCTGATGAAAGGGAT
CCTCGAGTCCCCATGCTGACCTGGAGCACCTGCGCTTTCACTATCCAGGC
AATTGAAAATCTATTGGGAGATGAAGGAAAACCTCTGTTTGGAGCACTTC
AAAATAGGCAGCATAATGGTCTGAAAGCATTAATGCAGTTTGCAGTTGCA
CAGAGGATTACCTGTCCTCAGGTCCTGATACAGAAACATCTGGTTCGTCT
TCTATCAGTTGTTCTTCCTAACATAAAATCAGAAGATACACCATGCCTTC
TGTCTATAGATCTGTTTCATGTTTTGGTGGGTGCTGTGTTAGCATTCCCA
TCCTTGTATTGGGATGACCCTGTTGATCTGCAGCCTTCTTCAGTTAGTTC
TTCCTATAACCACCTTTATCTCTTCCATTTGATCACCATGGCACACATGC
TTCAGATACTACTTACAGTAGACACAGGCCTACCCCTTGCTCAGGTTCAA
GAAGACAGTGAAGAGGCTCATTCCGCATCTTCTTTCTTTGCAGAAATTTC
TCAATATACAAGTGGCTCCATTGGGTGTGATATTCCTGGCTGGTATTTGT
GGGTCTCACTGAAGAATGGCATCACCCCTTATCTTCGCTGTGCTGCATTG
TTTTTCCACTATTTACTTGGGGTAACTCCGCCTGAGGAACTGCATACCAA
TTCTGCAGAAGGAGAGTACAGTGCACTCTGTAGCTATCTATCTTTACCTA
CAAATTTGTTCCTGCTCTTCCAGGAATATTGGGATACTGTAAGGCCCTTG
CTCCAGAGGTGGTGTGCAGATCCTGCCTTACTAAACTGTTTGAAGCAAAA
AAACACCGTGGTCAGGTACCCTAGAAAAAGAAATAGTTTGATAGAGCTTC
CTGATGACTATAGCTGCCTCCTGAATCAAGCTTCTCATTTCAGGTGCCCA
CGGTCTGCAGATGATGAGCGAAAGCATCCTGTCCTCTGCCTTTTCTGTGG
GGCTATACTATGTTCTCAGAACATTTGCTGCCAGGAAATTGTGAACGGGG
AAGAGGTTGGAGCTTGCATTTTTCACGCACTTCACTGTGGAGCCGGAGTC
TGCATTTTCCTAAAAATCAGAGAATGCCGAGTGGTCCTGGTTGAAGGTAA
AGCCAGAGGCTGTGCCTATCCAGCTCCTTACTTGGATGAATATGGAGAAA
CAGACCCTGGCCTGAAGAGGGGCAACCCCCTTCATTTATCTCGTGAGCGG
TATCGGAAGCTCCATTTGGTCTGGCAACAACACTGCATTATAGAAGAGAT
TGCTAGGAGCCAAGAGACTAATCAGATGTTATTTGGATTCAACTGGCAGT
TACTGTGAgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRRMADE
EAGGTERMEISAELPQTPQRLASWWDQQVDFYTAFLHHLAQLVPEIYFAE
MDPDLEKQEESVQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGRVFK
SGETTYSCRDCAIDPTCVLCMDCFQDSVHKNHRYKMHTSTGGGFCDCGDT
EAWKTGPFCVNHEPGRAGTIKENSRCPLNEEVIVQARKIFPSVIKYVVEM
TIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCEIA
EAQLHTTAIDKEGRRAVKAGAYAACQEAKEDIKSHSENVSQHPLHVEVLH
SEIMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPDSENPCLISR
LMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYI
SDDHDRSISITALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLD
RNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTIWTERLRMQFLEGFRS
FLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCA
CDEELLLVAYKECHKAVMRCSTSFISSSKTVVQSCGHSLETKSYRVSEDL
VSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVSFEDFQVEVLVEYPLRCLV
LVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDP
NKFLLLVLQRYELAEAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGER
YVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVI
NKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRR
KQENKDEALPPPPPPEFCPAFSKVINLLNCDIMMYILRTVFERAIDTDSN
LWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMN
IQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSES
IKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDN
TSEMPGKEDSIMEEESTPAVSDYSRIALGPKRGPSVTEKEVLTCILCQEE
QEVKIENNAMVLSACVQKSTALTQHRGKPIELSGEALDPLFMDPDLAYGT
YTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLC
NTVIPIIPLQPQKINSENADALAQLLTLARWIQTVLARISGYNIRHAKGE
NPIPIFFNQGMGDSTLEFHSILSFGVESSIKYSNSIKEMVILFATTIYRI
GLKVPPDERDPRVPMLTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNG
LKALMQFAVAQRITCPQVLIQKHLVRLLSVVLPNIKSEDTPCLLSIDLFH
VLVGAVLAFPSLYWDDPVDLQPSSVSSSYNHLYLFHLITMAHMLQILLTV
DTGLPLAQVQEDSEEAHSASSFFAEISQYTSGSIGCDIPGWYLWVSLKNG
ITPYLRCAALFFHYLLGVTPPEELHTNSAEGEYSALCSYLSLPTNLFLLF
QEYWDTVRPLLQRWCADPALLNCLKQKNTVVRYPRKRNSLIELPDDYSCL
LNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACI
FHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKR
GNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24020
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: (leu 253 Ile) substitution to be corrected. grown at 30 degrees