cDNA Clone ABIN1

• Plasmid: pcDNA5 FRT/TO GFP Abin1 siRNA resistant
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-ABIN1 siRNA resistant
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGAAGGGAGAGGACCGTACCGGATCTACGACCCTGGGGGCAGCGTGCCCTCAGGAGAGGCATCCGCAGCTTTTGAGCGCCTAGTGAAGGAGAATTCCCGGCTGAAGGAAAAAATGCAAGGGATAAAGATGTTAGGGGAGCTTTTGGAAGAGTCCCAGATGGAAGCGACCAGGCTCCGGCAGAAGGCAGAGGAGCTAGTGAAGGACAACGAGCTGCTCCCACCACCTTCTCCCTCCTTGGGCTCCTTCGACCCCCTGGCTGAGCTCACAGGAAAGGACTCAAATGTCACAGCATCTCCCACAGCCCCTGCATGCCCCAGTGACAAGCCAGCACCAGTCCAGAAGCCTCCATCCAGTGGCACCTCCTCTGAATTTGAAGTGGTCACTCCTGAGGAGCAGAATTCACCAGAGAGCAGCAGCCATGCCAATGCGATGGCGCTGGGCCCCCTGCCCCGTGAGGACGGCAACCTGATGCTGCACCTGCAGCGCCTGGAGACCACGCTGAGTGTGTGTGCCGAGGAGCCGGACCACGGCCAGCTCTTCACCCACCTGGGCCGCATGGCCCTGGAGTTCAACCGACTGGCATCCAAGGTGCACAAGAATGAGCAGCGCACCTCCATTCTGCAGACCCTGTGTGAGCAGCTTCGGAAGGAGAACGAGGCTCTGAAGGCCAAGTTGGATAAGGGCCTGGAACAGCGGGATCAGGCTGCCGAGAGGCTGCGGGAGGAAAATTTGGAGCTCAAGAAGTTGTTGATGAGCAATGGCAACAAAGAGGGTGCGTCTGGGCGGCCAGGCTCACCGAAGATGGAAGGGACAGGCAAGAAGGCAGTGGCTGGACAGCAGCAGGCTAGTGTGACGGCAGGTAAGGTCCCAGAGGTGGTGGCCTTGGGCGCACCCGAGAAGAAGGTGAAGATGCTGGAGCAGCAGCGCAGTGAGCTGCTGGAAGTGAACAAGCAGTGGGACCAGCATTTCCGGTCCATGAAGCAGCAGTATGAGCAGAAGATCACTGAGCTGCGTCAGAAGCTGGCTGATTTGCAGAAGCAGGTGACTGACCTGGAGGCCGAGCGGGAGCAGAAGCAGCGTGACTTTGACCGCAAGCTCCTCCTGGCCAAGTCCAAGATTGAAATGGAGGAGACCGACAAGGAGCAGCTGACAGCAGAGGCCAAGGAGCTGCGCCAAAAGGTCAAGTACCTGCAGGATCAGCTGAGCCCACTCACCCGACAGCGTGAGTACCAGGAAAAGGAGATCCAGCGGCTCAACAAGGCCCTGGAGGAAGCACTGAGCATCCAAACCCCGCCATCATCTCCACCAACAGCATTTGGGAGCCCAGAAGGAGCAGGGGCCCTCCTAAGGAAACAGGAGCTGGTCACGCAGAATGAGTTGCTGAAACAGCAGGTGAAGATCTTCGAGGAGGACTTCCAGAGGGAGCGCAGTGATCGTGAGCGCATGAATGAGGAGAAGGAAGAGCTGAAGAAGCAAGTGGAGAAGCTGCAGGCCCAGGTCACCCTGTCAAATGCCCAGCTAAAAGCATTCAAAGATGAGGAGAAGGCGAGGGAGGCTCTTAGGCAGCAGAAGAGGAAAGCAAAGGCCTCAGGAGAGCGTTACCATGTGGAGCCCCACCCAGAACATCTCTGCGGGGCCTACCCCTACGCCTACCCGCCCATGCCAGCCATGGTGCCACACCATGGCTTCGAGGACTGGTCCCAGATCCGCTACCCCCCTCCCCCCATGGCCATGGAGCACCCGCCCCCACTCCCCAACTCGCGCCTCTTCCATCTGCCGGAATACACCTGGCGTCTACCTTGTGGAGGGGTTCGAAATCCAAATCAGAGCTCCCAAGTGATGGACCCTCCCACAGCCAGGCCTACAGAACCAGAGTCTCCAAAAAATGACCGTGAGGGGCCTCAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMEGRGP
YRIYDPGGSVPSGEASAAFERLVKENSRLKEKMQGIKMLGELLEESQMEA
TRLRQKAEELVKDNELLPPPSPSLGSFDPLAELTGKDSNVTASPTAPACP
SDKPAPVQKPPSSGTSSEFEVVTPEEQNSPESSSHANAMALGPLPREDGN
LMLHLQRLETTLSVCAEEPDHGQLFTHLGRMALEFNRLASKVHKNEQRTS
ILQTLCEQLRKENEALKAKLDKGLEQRDQAAERLREENLELKKLLMSNGN
KEGASGRPGSPKMEGTGKKAVAGQQQASVTAGKVPEVVALGAPEKKVKML
EQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQKLADLQKQVTDLEAER
EQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLT
RQREYQEKEIQRLNKALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELV
TQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLSNA
QLKAFKDEEKAREALRQQKRKAKASGERYHVEPHPEHLCGAYPYAYPPMP
AMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNSRLFHLPEYTWRLPCGGVR
NPNQSSQVMDPPTARPTEPESPKNDREGPQ*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25039
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has the following silent changes ; A519 change GCA to GCG, ; R520 change AGA to AGG, ; E521 change GAA to GAG, ; A522 change GCC to GCT, ; L523 change CTC to CTT, ; R524 change AGA to AGG