cDNA Clone FANCI

• Synonyms: Fanconi anemia, complementation group I, KIAA1794
• Plasmid: pcDNA5D FRT/TO-Flag-FANCI K898E K900E K980E
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: Flag-FANCI K898E K900E K980E
• Genbank: NM_001113378.1.
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGACCAGAAGATTTTATCTCTAGCAGCAGAAAAAACAGCAGACAAACT
GCAAGAATTTCTTCAAACCCTGAGAGAAGGTGATTTGACTAATCTCCTTC
AGAATCAAGCAGTGAAAGGAAAAGTTGCTGGAGCACTCCTGAGAGCCATC
TTCAAAGGTTCCCCCTGCTCTGAGGAAGCTGGAACACTTAGGAGACGTAA
GATATACACTTGTTGTATCCAGTTGGTGGAATCGGGGGATTTGCAGAAAG
AAATAGCGTCTGAGATCATAGGATTACTGATGCTGGAGGCTCACCATTTT
CCAGGACCATTATTGGTTGAATTAGCCAATGAGTTTATTAGTGCTGTCAG
AGAAGGCAGCCTAGTGAATGGAAAATCTTTGGAGTTACTACCTATCATTC
TCACTGCCCTGGCTACGAAAAAGGAAAATCTGGCTTATGGAAAAGGTGTA
CTGAGTGGGGAAGAATGTAAGAAACAGTTGATTAACACCCTGTGTTCTGG
CAGGTGGGATCAGCAATATGTAATCCAACTCACCTCCATGTTCAAGGATG
TCCCTCTGACTGCAGAAGAGGTGGAATTTGTGGTGGAAAAAGCATTGAGC
ATGTTCTCCAAGATGAATCTTCAAGAAATACCACCTTTGGTCTATCAGCT
TCTGGTTCTCTCCTCCAAGGGAAGCAGAAAGAGTGTTTTGGAAGGAATCA
TAGCCTTCTTCAGTGCACTAGATAAGCAGCACAATGAGGAACAGAGTGGT
GACGAGCTATTGGATGTTGTCACTGTGCCATCAGGTGAACTTCGTCATGT
GGAAGGCACCATTATTCTACACATTGTGTTTGCCATCAAATTGGACTATG
AACTAGGCAGAGAACTCGTGAAACACTTAAAGGTAGGACAGCAAGGAGAT
TCCAATAATAACTTAAGTCCCTTCAGCATTGCTCTTCTTCTGTCTGTAAC
AAGAATACAAAGATTTCAGGACCAGGTGCTTGATCTTTTAAAGACTTCGG
TTGTAAAGAGCTTTAAGGATCTTCAACTCCTCCAAGGCTCAAAATTTCTT
CAGAATCTAGTTCCTCATAGATCTTATGTTTCAACCATGATCTTGGAAGT
AGTGAAGAATAGCGTTCATAGCTGGGACCATGTTACTCAGGGCCTCGTAG
AACTTGGTTTCATTTTGATGGATTCATATGGGCCAAAGAAGGTTCTTGAT
GGAAAAACTATTGAAACCAGCCCAAGTCTTTCTAGAATGCCAAACCAGCA
TGCATGTAAGCTCGGAGCTAATATCCTGTTGGAAACTTTTAAGATCCATG
AGATGATCAGACAAGAAATTTTGGAGCAGGTCCTCAACAGGGTTGTTACC
AGAGCATCTTCTCCCATCAGTCATTTCTTAGACCTGCTTTCAAATATCGT
CATGTATGCACCCTTAGTTCTTCAAAGTTGTTCTTCTAAAGTCACAGAAG
CTTTTGACTATTTGTCCTTTCTGCCCCTTCAGACTGTACAAAGGCTGCTT
AAGGCAGTGCAGCCCCTTCTCAAAGTCAGCATGTCAATGAGAGACTGCTT
GATACTTGTCCTTCGGAAAGCTATGTTTGCCAACCAGCTTGATGCCCGAA
AATCTGCAGTTGCTGGGTTTTTGCTGCTCCTGAAGAACTTTAAAGTTTTA
GGCAGCCTGTCATCCTCTCAGTGCAGTCAGTCTCTCAGTGTCAGTCAGGT
TCATGTGGATGTTCACAGCCATTACAATTCTGTCGCCAATGAAACTTTTT
GCCTTGAGATCATGGATAGTTTGAGGAGATGCTTAAGCCAGCAAGCTGAT
GTTCGACTCATGCTTTATGAGGGGTTTTATGATGTTCTTCGAAGGAACTC
TCAGCTGGCTAATTCAGTCATGCAAACTCTGCTCTCACAGTTAAAACAGT
TCTATGAGCCAAAACCTGATCTGCTGCCTCCTCTGAAATTAGAAGCTTGT
ATTCTGACCCAAGGAGATAAGATCTCTCTACAAGAACCACTGGATTATCT
GCTGTGTTGTATTCAGCATTGTTTGGCCTGGTATAAGAATACAGTCATAC
CCTTACAGCAGGGAGAGGAGGAAGAGGAGGAGGAAGAGGCATTCTACGAA
GACCTAGATGATATATTGGAGTCCATTACTAATAGAATGATTAAGAGTGA
GCTGGAAGACTTTGAACTGGATAAATCAGCAGATTTTTCTCAGAGCACCA
GTATTGGCATAAAAAATAATATCTGTGCTTTTCTTGTGATGGGAGTTTGT
GAGGTTTTAATAGAATACAATTTCTCCATAAGTAGTTTCAGTAAGAATAG
GTTTGAGGACATTCTGAGCTTATTTATGTGTTACAAAAAACTCTCTGACA
TTCTTAATGAAAAAGCGGGTAAAGCCAAAACTAAAATGGCCAACAAGACA
AGTGATAGTCTTTTGTCCATGAAATTTGTGTCCAGTCTTCTCACTGCTCT
TTTCAGGGATAGTATCCAAAGCCACCAAGAAAGCCTTTCTGTTCTCAGGT
CCAGCAATGAGTTTATGCGCTATGCAGTGAATGTAGCTCTGCAGAAAGTA
CAGCAGCTAAAGGAAACAGGGCATGTGAGTGGCCCTGATGGCCAAAACCC
AGAAAAGATCTTTCAGAACCTCTGTGACATAACTCGAGTCTTGCTATGGA
GATACACTTCAATTCCTACTTCAGTGGAAGAGTCGGGAAAGGAAGAGGAA
GGAAAGAGCATCTCACTGCTGTGCTTGGAGGGTTTACAGAAAATATTCAG
TGCTGTGCAACAGTTCTATCAGCCCAAGATTCAGCAGTTTCTCAGAGCTC
TGGATGTCACAGATAAGGAAGGAGAAGAGAGAGAAGATGCAGATGTCAGT
GTCACTCAGAGAACAGCATTCCAGATCCGGCAATTTCAGAGGTCCTTGTT
GAATTTACTTAGCAGTCAAGAGGAAGATTTTAATAGCGAAGAAGCCCTCC
TGCTAGTCACGGTTCTTACCAGTTTGTCCAAGTTACTGGAGCCCTCCTCT
CCTCAGTTTGTGCAGATGTTATCCTGGACATCAAAGATTTGCAAGGAAAA
CAGCCGGGAGGATGCCTTGTTTTGCAAGAGCTTGATGAACTTGCTCTTCA
GCCTGCATGTTTCGTATAAGAGTCCTGTCATTCTGCTGCGTGACTTGTCC
CAGGATATCCACGGGCATCTGGGAGATATAGACCAGGATGTAGAGGTGGA
GAAAACAAACCACTTTGCAATAGTGAATTTGAGAACGGCTGCCCCCACTG
TCTGTTTACTTGTTCTGAGTCAGGCCGAGAAGGTTCTAGAAGAAGTGGAC
TGGCTAATCACCAAGCTTAAGGGACAAGTGAGCCAAGAAACCTTATCAGA
AGAGGCCTCTTCTCAGGCAACCCTACCAAATCAGCCTGTTGAGAAAGCTA
TCATCATGCAACTGGGAACTCTGCTTACATTTTTCCACGAGCTGGTGCAG
ACAGCTCTGCCATCAGGCAGCTGTGTGGACACCTTGTTAAAGGACTTGTG
CAAAATGTACACCACACTTACAGCCCTTGTCAGATATTATCTCCAGGTGT
GTCAGAGCTCCGGAGGAATTCCAAAAAATATGGAAAAGCTGGTGAAGCTG
TCTGGTTCTCATCTGACCCCCCTGTGTTATTCTTTCATTTCTTACGTACA
GAATAAGAGTAAGAGCCTGAACTATACGGGAGAGAAAAAGGAGAAACCTG
CTGCCGTTGCCACAGCCATGGCCAGAGTTCTTCGGGAAACCAAGCCAATC
CCTAACCTCATCTTTGCCATAGAACAGTATGAAAAATTTCTCATCCACCT
TTCTAAGAAGTCCAAGGTGAACCTGATGCAGCACATGAAGCTCAGCACCT
CACGAGACTTCAAGATCAAAGGAAACATCCTAGACATGGTTCTTCGAGAG
GATGGTGAAGATGAAAATGAAGAGGGCACTGCATCAGAGCATGGGGGACA
GAACAAAGAACCAGCCAAGAAGAAAAGGAAAAAATAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVK
GKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIASEI
IGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALAT
KKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAE
EVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSA
LDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGREL
VKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFK
DLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFIL
MDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQE
ILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLS
FLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAG
FLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLEIMD
SLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKP
DLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQGE
EEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKN
NICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKA
GKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFM
RYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIP
TSVEESGKEEEGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDK
EGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSEEALLLVTVL
TSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHVSY
KSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVL
SQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLG
TLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGG
IPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATA
MARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSRDFKI
KGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK*AA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24035
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All FANCI plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Parental 41457