cDNA Clone FANCI

• Plasmid: pcDNA5-FRT/TO-Flag-FANCI S556D/S559D/S565D/S596D R1285Q
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: Flag-FANCI S556D/S559D/S565D/S596D R1285Q
• Genbank: NM_001113378.1.
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCatggaccagaagattttatctctagcagcagaaaaaacagcaga
caaactgcaagaatttcttcaaaccctgagagaaggtgatttgactaatc
tccttcagaatcaagcagtgaaaggaaaagttgctggagcactcctgaga
gccatcttcaaaggttccccctgctctgaggaagctggaacacttaggag
acgtaagatatacacttgttgtatccagttggtggaatcgggggatttgc
agaaagaaatagcgtctgagatcataggattactgatgctggaggctcac
cattttccaggaccattattggttgaattagccaatgagtttattagtgc
tgtcagagaaggcagcctagtgaatggaaaatctttggagttactaccta
tcattctcactgccctggctacgaaaaaggaaaatctggcttatggaaaa
ggtgtactgagtggggaagaatgtaagaaacagttgattaacaccctgtg
ttctggcaggtgggatcagcaatatgtaatccaactcacctccatgttca
aggatgtccctctgactgcagaagaggtggaatttgtggtggaaaaagca
ttgagcatgttctccaagatgaatcttcaagaaataccacctttggtcta
tcagcttctggttctctcctccaagggaagcagaaagagtgttttggaag
gaatcatagccttcttcagtgcactagataagcagcacaatgaggaacag
agtggtgacgagctattggatgttgtcactgtgccatcaggtgaacttcg
tcatgtggaaggcaccattattctacacattgtgtttgccatcaaattgg
actatgaactaggcagagaactcgtgaaacacttaaaggtaggacagcaa
ggagattccaataataacttaagtcccttcagcattgctcttcttctgtc
tgtaacaagaatacaaagatttcaggaccaggtgcttgatcttttaaaga
cttcggttgtaaagagctttaaggatcttcaactcctccaaggctcaaaa
tttcttcagaatctagttcctcatagatcttatgtttcaaccatgatctt
ggaagtagtgaagaatagcgttcatagctgggaccatgttactcagggcc
tcgtagaacttggtttcattttgatggattcatatgggccaaagaaggtt
cttgatggaaaaactattgaaaccagcccaagtctttctagaatgccaaa
ccagcatgcatgtaagctcggagctaatatcctgttggaaacttttaaga
tccatgagatgatcagacaagaaattttggagcaggtcctcaacagggtt
gttaccagagcatcttctcccatcagtcatttcttagacctgctttcaaa
tatcgtcatgtatgcacccttagttcttcaaagttgttcttctaaagtca
cagaagcttttgactatttgtcctttctgccccttcagactgtacaaagg
ctgcttaaggcagtgcagccccttctcaaagtcagcatgtcaatgagaga
ctgcttgatacttgtccttcggaaagctatgtttgccaaccagcttgatg
cccgaaaatctgcagttgctgggtttttgctgctcctgaagaactttaaa
gttttaggcagcctgtcatccGAtcagtgcGAtcagtctctcagtgtcGA
tcaggttcatgtggatgttcacagccattacaattctgtcgccaatgaaa
ctttttgccttgagatcatggatagtttgaggagatgcttaGAccagcaa
gctgatgttcgactcatgctttatgaggggttttatgatgttcttcgaag
gaactctcagctggctaattcagtcatgcaaactctgctctctcagttaa
aacagttctatgagccaaaacctgatctgctgcctcctctgaaattagaa
gcttgtattctgacccaaggagataagatctctctacaagaaccactgga
ttatctgctgtgttgtattcagcattgtttggcctggtataagaatacag
tcatacccttacagcagggagaggaggaagaggaggaggaagaggcattc
tacgaagacctagatgatatattggagtccattactaatagaatgattaa
gagtgagctggaagactttgaactggataaatcagcagatttttctcaga
gcaccagtattggcataaaaaataatatctgtgcttttcttgtgatggga
gtttgtgaggttttaatagaatacaatttctccataagtagtttcagtaa
gaataggtttgaggacattctgagcttatttatgtgttacaaaaaactct
ctgacattcttaatgaaaaagcgggtaaagccaaaactaaaatggccaac
aagacaagtgatagtcttttgtccatgaaatttgtgtccagtcttctcac
tgctcttttcagggatagtatccaaagccaccaagaaagcctttctgttc
tcaggtccagcaatgagtttatgcgctatgcagtgaatgtagctctgcag
aaagtacagcagctaaaggaaacagggcatgtgagtggccctgatggcca
aaacccagaaaagatctttcagaacctctgtgacataactcgagtcttgc
tatggagatacacttcaattcctacttcagtggaagagtcgggaaagaaa
gagaaaggaaagagcatctcactgctgtgcttggagggtttacagaaaat
attcagtgctgtgcaacagttctatcagcccaagattcagcagtttctca
gagctctggatgtcacagataaggaaggagaagagagagaagatgcagat
gtcagtgtcactcagagaacagcattccagatccggcaatttcagaggtc
cttgttgaatttacttagcagtcaagaggaagattttaatagcaaagaag
ccctcctgctagtcacggttcttaccagtttgtccaagttactggagccc
tcctctcctcagtttgtgcagatgttatcctggacatcaaagatttgcaa
ggaaaacagccgggaggatgccttgttttgcaagagcttgatgaacttgc
tcttcagcctgcatgtttcgtataagagtcctgtcattctgctgcgtgac
ttgtcccaggatatccacgggcatctgggagatatagaccaggatgtaga
ggtggagaaaacaaaccactttgcaatagtgaatttgagaacggctgccc
ccactgtctgtttacttgttctgagtcaggccgagaaggttctagaagaa
gtggactggctaatcaccaagcttaagggacaagtgagccaagaaacctt
atcagaagaggcctcttctcaggcaaccctaccaaatcagcctgttgaga
aagctatcatcatgcaactgggaactctgcttacatttttccacgagctg
gtgcagacagctctgccatcaggcagctgtgtggacaccttgttaaagga
cttgtgcaaaatgtacaccacacttacagcccttgtcagatattatctcc
aggtgtgtcagagctccggaggaattccaaaaaatatggaaaagctggtg
aagctgtctggttctcatctgacccccctgtgttattctttcatttctta
cgtacagaataagagtaagagcctgaactatacgggagagaaaaaggaga
aacctgctgccgttgccacagccatggccagagttcttcgggaaaccaag
ccaatccctaacctcatctttgccatagaacagtatgaaaaatttctcat
ccacctttctaagaagtccaaggtgaacctgatgcagcacatgaagctca
gcacctcacaagacttcaagatcaaaggaaacatcctagacatggttctt
cgagaggatggtgaagatgaaaatgaagagggcactgcatcagagcatgg
gggacagaacaaagaaccagccaagaagaaaaggaaaaaataaGCGGCCG
C

Amino Acid Sequence

MDYKDDDDKGSMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVK
GKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIASEI
IGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALAT
KKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAE
EVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSA
LDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGREL
VKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFK
DLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFIL
MDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQE
ILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLS
FLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAG
FLLLLKNFKVLGSLSSDQCDQSLSVDQVHVDVHSHYNSVANETFCLEIMD
SLRRCLDQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKP
DLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQGE
EEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKN
NICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKA
GKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFM
RYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIP
TSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDK
EGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALLLVTVL
TSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHVSY
KSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVL
SQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLG
TLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGG
IPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATA
MARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSQDFKI
KGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU24037
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All FANCI plasmids MUST be grown at 30 degrees C or less to prevent recombination.