cDNA Clone HPK1

• Synonyms: MAP4K1
• Plasmid: pET28a DAC-Thrombin HPK1
• Parent Plasmid: pET28a DAC Thr
• Expressed: DAC-Thrombin HPK1
• Genbank: NM_001042600.1 GI:110611907 with silent changes c1569t and a2127g
• Tag: DAC
• Species: Human

Sequence of Insert

AGATCTATGGACGTCGTGGACCCTGACATTTTCAATAGAGACCCCCGGGACCACTATGACCTGCTACAGCGGCTGGGTGGCGGCACGTATGGGGAAGTCTTTAAGGCTCGAGACAAGGTGTCAGGGGACCTGGTGGCACTGAAGATGGTGAAGATGGAGCCTGATGATGATGTCTCCACCCTTCAGAAGGAAATCCTCATATTGAAAACTTGCCGGCACGCCAACATCGTGGCCTACCATGGGAGTTATCTCTGGTTGCAGAAACTCTGGATCTGCATGGAATTCTGTGGGGCTGGTTCTCTCCAGGACATCTACCAAGTGACAGGCTCCCTGTCAGAGCTCCAGATTAGCTATGTCTGCCGGGAAGTGCTCCAGGGACTGGCCTATTTGCACTCACAGAAGAAGATACACAGGGACATCAAGGGAGCTAACATCCTCATCAATGATGCTGGGGAGGTCAGATTGGCTGACTTTGGCATCTCGGCCCAGATTGGGGCTACACTGGCCAGACGCCTCTCTTTCATTGGGACACCCTACTGGATGGCTCCGGAAGTGGCAGCTGTGGCCCTGAAGGGAGGATACAATGAGCTGTGTGACATCTGGTCCCTGGGCATCACGGCCATCGAACTGGCCGAGCTACAGCCACCGCTCTTTGATGTGCACCCTCTCAGAGTTCTCTTCCTCATGACCAAGAGTGGCTACCAGCCTCCCCGACTGAAGGAAAAAGGCAAATGGTCGGCTGCCTTCCACAACTTCATCAAAGTCACTCTGACTAAGAGTCCCAAGAAACGACCCAGCGCCACCAAGATGCTCAGTCATCAACTGGTATCCCAGCCTGGGCTGAATCGAGGCCTGATCCTGGATCTTCTTGACAAACTGAAGAATCCCGGGAAAGGACCCTCCATTGGGGACATTGAGGATGAGGAGCCCGAGCTACCCCCTGCTATCCCTCGGCGGATCAGATCCACCCACCGCTCCAGCTCTCTGGGGATCCCAGATGCAGACTGCTGTCGGCGGCACATGGAGTTCAGGAAGCTCCGAGGAATGGAGACCAGACCCCCAGCCAACACCGCTCGCCTACAGCCTCCTCGAGACCTCAGGAGCAGCAGCCCCAGGAAGCAACTGTCAGAGTCGTCTGACGATGACTATGACGACGTGGACATCCCCACCCCTGCAGAGGACACACCTCCTCCACTTCCCCCCAAGCCCAAGTTCCGTTCTCCATCAGACGAGGGTCCTGGGAGCATGGGGGATGATGGGCAGCTGAGCCCGGGGGTGCTGGTCCGGTGTGCCAGTGGGCCCCCACCAAACAGCCCCCGTCCTGGGCCTCCCCCATCCACCAGCAGCCCCCACCTCACCGCCCATTCAGAACCCTCACTCTGGAACCCACCCTCCCGGGAGCTTGACAAGCCCCCACTTCTGCCCCCCAAGAAGGAAAAGATGAAGAGAAAGGGATGTGCCCTTCTCGTAAAGTTGTTCAATGGCTGCCCCCTCCGGATCCACAGCACGGCCGCCTGGACACATCCCTCCACCAAGGACCAGCACCTGCTCCTGGGGGCAGAGGAAGGCATCTTTATCCTGAACCGGAATGACCAGGAGGCCACGCTGGAAATGCTCTTTCCTAGCCGGACTACGTGGGTGTACTCCATCAACAACGTTCTCATGTCTCTCTCAGGAAAGACCCCCCACCTGTATTCTCATAGCATCCTTGGCCTGCTGGAACGGAAAGAGACCAGAGCAGGAAACCCCATCGCTCACATTAGCCCCCACCGCCTACTGGCAAGGAAGAACATGGTTTCCACCAAGATCCAGGACACCAAAGGCTGCCGGGCGTGCTGTGTGGCGGAGGGTGCGAGCTCTGGGGGCCCGTTCCTGTGCGGTGCATTGGAGACGTCCGTTGTCCTGCTTCAGTGGTACCAGCCCATGAACAAATTCCTGCTTGTCCGGCAGGTGCTGTTCCCACTGCCGACGCCTCTGTCCGTGTTCGCGCTGCTGACCGGGCCAGGCTCTGAGCTGCCCGCTGTGTGCATCGGCGTGAGCCCCGGGCGGCCGGGGAAGTCGGTGCTCTTCCACACGGTGCGCTTTGGCGCGCTCTCTTGCTGGCTGGGCGAGATGAGCACCGAGCACAGGGGGCCCGTGCAGGTGACCCAGGTAGAGGAAGATATGGTGATGGTGTTGATGGATGGCTCTGTGAAGCTGGTGACCCCGGAGGGGTCCCCAGTCCGGGGACTTCGCACACCTGAGATCCCCATGACCGAAGCGGTGGAGGCCGTGGCTATGGTTGGAGGTCAGCTTCAGGCCTTCTGGAAGCATGGAGTGCAGGTGTGGGCTCTAGGCTCGGATCAGCTGCTACAGGAGCTGAGAGACCCTACCCTCACTTTCCGTCTGCTTGGCTCCCCCAGGCCTGTAGTGGTGGAGACACGCCCAGTGGATGATCCTACTGCTCCCAGCAACCTCTACATCCAGGAATGAGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPLVPRGSGSMDVVDPDIFNRDPRDHYDLLQRLGGGTYG
EVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHG
SYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLH
SQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWM
APEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTK
SGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGL
NRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGI
PDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDD
DYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCA
SGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKM
KRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRND
QEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAG
NPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALET
SVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSP
GRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSV
KLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSD
QLLQELRDPTLTFRLLGSPRPVVVETRPVDDPTAPSNLYIQE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45597
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 21714