cDNA Clone cyclin T1

• Synonyms: CCNT1
• Plasmid: pET156P Cyclin T1
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-cyclin T1
• Genbank: NM_001240.2 GI:17978465 with silent changes t453c, c1341g, c2034t
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGAGGGAGAGAGGAAGAACAACAACAAACGGTGGTATTTCACTCGAGAACAGCTGGAAAATAGCCCATCCCGTCGTTTTGGCGTGGACCCAGATAAAGAACTTTCTTATCGCCAGCAGGCGGCCAATCTGCTTCAGGACATGGGGCAGCGTCTTAACGTCTCACAATTGACTATCAACACTGCTATAGTATACATGCATCGATTCTACATGATTCAGTCCTTCACACAGTTCCCTGGAAATTCTGTGGCTCCAGCAGCCTTGTTTCTAGCAGCTAAAGTGGAGGAGCAGCCCAAAAAATTGGAACATGTCATCAAGGTAGCACATACTTGTCTCCATCCTCAGGAATCCCTTCCTGATACTAGAAGTGAGGCTTATTTGCAACAAGTTCAAGATCTGGTCATTTTAGAAAGCATAATTTTGCAGACTTTAGGCTTTGAACTAACAATTGACCACCCACATACTCATGTAGTAAAGTGCACTCAACTTGTTCGAGCAAGCAAGGACTTAGCACAGACTTCTTACTTCATGGCAACCAACAGCCTGCATTTGACCACATTTAGCCTGCAGTACACACCTCCTGTGGTGGCCTGTGTCTGCATTCACCTGGCTTGCAAGTGGTCCAATTGGGAGATCCCAGTCTCAACTGACGGGAAGCACTGGTGGGAGTATGTTGACGCCACTGTGACCTTGGAACTTTTAGATGAACTGACACATGAGTTTCTACAGATTTTGGAGAAAACTCCCAACAGGCTCAAACGCATTTGGAATTGGAGGGCATGCGAGGCTGCCAAGAAAACAAAAGCAGATGACCGAGGAACAGATGAAAAGACTTCAGAGCAGACAATCCTCAATATGATTTCCCAGAGCTCTTCAGACACAACCATTGCAGGTTTAATGAGCATGTCAACTTCTACCACAAGTGCAGTGCCTTCCCTGCCAGTCTCCGAAGAGTCATCCAGCAACTTAACCAGTGTGGAGATGTTGCCGGGCAAGCGTTGGCTGTCCTCCCAACCTTCTTTCAAACTAGAACCTACTCAGGGTCATCGGACTAGTGAGAATTTAGCACTTACAGGAGTTGATCATTCCTTACCACAGGATGGTTCAAATGCATTTATTTCCCAGAAGCAGAATAGTAAGAGTGTGCCATCAGCTAAAGTGTCACTGAAAGAATACCGCGCGAAGCATGCAGAAGAATTGGCTGCCCAGAAGAGGCAACTGGAGAACATGGAAGCCAATGTGAAGTCACAATATGCATATGCTGCCCAGAATCTCCTTTCTCATCATGATAGCCATTCTTCAGTCATTCTAAAAATGCCCATAGAGGGTTCAGAAAACCCGGAGCGGCCTTTTCTGGAAAAGGCTGACAAAACAGCTCTCAAAATGAGAATCCCAGTGGCAGGTGGAGATAAAGCTGCGTCTTCAAAACCAGAGGAGATAAAAATGCGCATAAAAGTCCATGCTGCAGCTGATAAGCACAATTCTGTAGAGGACAGTGTTACAAAGAGCCGAGAGCACAAAGAAAAGCACAAGACTCACCCATCTAATCATCATCATCATCATAATCACCACTCACACAAGCACTCTCATTCCCAACTTCCAGTTGGTACTGGGAACAAACGTCCTGGTGATCCAAAACATAGTAGCCAGACAAGCAACTTAGCACATAAAACCTATAGCTTGTCTAGTTCTTTTTCCTCTTCCAGTTCTACTCGTAAAAGGGGACCCTCTGAAGAGACTGGAGGGGCTGTGTTTGATCATCCAGCCAAGATTGCCAAGAGTACTAAATCCTCTTCCCTAAATTTCTCCTTCCCTTCACTTCCTACAATGGGTCAGATGCCTGGGCATAGCTCAGACACAAGTGGCCTTTCCTTTTCACAGCCCAGCTGTAAAACTCGTGTCCCTCATTCGAAACTGGATAAAGGGCCCACTGGGGCCAATGGTCACAACACGACCCAGACAATAGACTATCAAGACACTGTGAATATGCTTCACTCCCTGCTCAGTGCCCAGGGTGTTCAGCCCACTCAGCCTACTGCATTTGAATTTGTTCGTCCTTATAGTGACTATCTGAATCCTCGGTCTGGTGGAATCTCCTCGAGATCTGGCAATACAGACAAACCCCGGCCACCACCTCTGCCATCAGAACCTCCTCCACCACTTCCACCCCTTCCTAAGTAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMEGERKNNNKRWYFTREQLENSPSRRF
GVDPDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRFYMIQSFTQ
FPGNSVAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLPDTRSEAYL
QQVQDLVILESIILQTLGFELTIDHPHTHVVKCTQLVRASKDLAQTSYFM
ATNSLHLTTFSLQYTPPVVACVCIHLACKWSNWEIPVSTDGKHWWEYVDA
TVTLELLDELTHEFLQILEKTPNRLKRIWNWRACEAAKKTKADDRGTDEK
TSEQTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEESSSNLTSVE
MLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGSNAFISQKQ
NSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAYAAQNLLS
HHDSHSSVILKMPIEGSENPERPFLEKADKTALKMRIPVAGGDKAASSKP
EEIKMRIKVHAAADKHNSVEDSVTKSREHKEKHKTHPSNHHHHHNHHSHK
HSHSQLPVGTGNKRPGDPKHSSQTSNLAHKTYSLSSSFSSSSSTRKRGPS
EETGGAVFDHPAKIAKSTKSSSLNFSFPSLPTMGQMPGHSSDTSGLSFSQ
PSCKTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLSAQGVQPTQ
PTAFEFVRPYSDYLNPRSGGISSRSGNTDKPRPPPLPSEPPPPLPPLPK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU45598
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Urgent cloning for AZ project