cDNA Clone MUS81

• Plasmid: pcDNA5-FRT/TO-FLAG MUS81 sprot L66A Q67A
• Parent Plasmid: pcDNA5 FRT/TO FLAG
• Expressed: FLAG-MUS81 sprot L66A Q67A
• Genbank: NM_025128.4
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCCCGGTCCGCCTGGGCCGGAAGCGCCCGCTGCCTGCCTGTCCCAACCCGCTCTTCGTTCGCTGGCTGACCGAGTGGCGGGACGAGGCGACCCGCAGCAGGCACCGCACGCGCTTCGTATTTCAGAAGGCGCTGCGTTCCCTCCGACGGTACCCACTGCCGCTGCGCAGCGGGAAGGAAGCTAAGATCGCAGCGCACTTCGGAGACGGGCTCTGCCGGATGCTGGACGAGCGGCTGCAGCGGCACCGAACATCGGGCGGTGACCATGCCCCGGACTCACCATCTGGAGAGAACAGTCCAGCCCCGCAGGGGCGACTTGCGGAAGTCCAGGACTCTTCCATGCCAGTTCCTGCCCAGCCCAAAGCGGGAGGCTCTGGCAGCTACTGGCCAGCTCGGCACTCAGGAGCCCGAGTGATACTGCTGGTGCTCTACCGGGAGCACCTGAATCCTAATGGTCACCACTTCTTAACCAAGGAGGAGCTGCTGCAGAGGTGTGCTCAGAAGTCCCCCAGGGTAGCCCCTGGGAGTGCCCCACCCTGGCCAGCCCTCCGCTCCCTCCTTCACAGGAACCTGGTCCTCAGGACACACCAGCCAGCCAGGTACTCATTGACCCCAGAGGGCCTGGAGCTGGCCCAGAAGTTGGCCGAGTCAGAAGGCCTGAGCTTGCTGAATGTGGGCATCGGGCCCAAGGAGCCCCCTGGGGAGGAGACAGCAGTGCCAGGAGCAGCTTCAGCAGAGCTTGCCAGTGAAGCAGGGGTCCAGCAGCAGCCACTGGAGCTGAGGCCTGGAGAGTACAGGGTGCTGTTGTGTGTGGACATTGGCGAGACCCGGGGGGGCGGGCACAGGCCGGAGCTGCTCCGAGAGCTACAGCGGCTGCACGTGACCCACACGGTGCGCAAGCTGCACGTTGGAGATTTTGTGTGGGTGGCTCAGGAGACCAACCCTAGAGACCCAGCAAACCCTGGGGAGTTGGTACTGGATCACATTGTGGAGCGCAAGCGACTGGATGACCTTTGCAGCAGCATCATCGACGGCCGCTTCCGGGAGCAGAAGTTCCGACTGAAGCGCTGTGGTCTGGAGCGCCGGGTATACCTGGTGGAAGAGCATGGTTCCGTCCACAACCTCAGCCTTCCTGAGAGCACACTGCTGCAGGCTGTCACCAACACTCAGGTCATTGATGGCTTTTTTGTGAAGCGCACAGCAGACATTAAGGAGTCAGCCGCCTACCTGGCCCTCTTGACTCGGGGCCTGCAGAGACTCTACCAGGGCCACACCCTACGCAGCCGCCCCTGGGGAACCCCTGGGAACCCTGAATCAGGGGCCATGACCTCTCCAAACCCTCTCTGCTCACTCCTCACCTTCAGTGACTTCAACGCAGGAGCCATCAAGAATAAGGCCCAGTCGGTGCGAGAAGTGTTTGCCCGGCAGCTGATGCAGGTGCGCGGAGTGAGTGGGGAGAAGGCAGCTGCATTAGTAGACAGGTACAGCACCCCTGCCAGCCTCCTGGCCGCCTATGATGCCTGTGCCACCCCCAAGGAACAAGAGACACTGCTGAGCACCATCAAATGCGGAAGGTTGCAGAGGAATCTGGGGCCTGCTCTGAGCAGGACCTTATCCCAGCTCTACTGCAGCTACGGCCCCTTGACCTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMAAPVRLGRKRPLPACPNPLFVRWLTEWRDEATRSRHRT
RFVFQKALRSLRRYPLPLRSGKEAKIAAHFGDGLCRMLDERLQRHRTSGG
DHAPDSPSGENSPAPQGRLAEVQDSSMPVPAQPKAGGSGSYWPARHSGAR
VILLVLYREHLNPNGHHFLTKEELLQRCAQKSPRVAPGSAPPWPALRSLL
HRNLVLRTHQPARYSLTPEGLELAQKLAESEGLSLLNVGIGPKEPPGEET
AVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRGGGHRPELLR
ELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDD
LCSSIIDGRFREQKFRLKRCGLERRVYLVEEHGSVHNLSLPESTLLQAVT
NTQVIDGFFVKRTADIKESAAYLALLTRGLQRLYQGHTLRSRPWGTPGNP
ESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEK
AAALVDRYSTPASLLAAYDACATPKEQETLLSTIKCGRLQRNLGPALSRT
LSQLYCSYGPLT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45615
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SiRNA protected against:; MUS81 siRNA #9: 5'-CAGCCCTGGTGGATCGATA-3' ; MUS81 siRNA #10: 5'-CATTAAGTGTGGGCGTCTA-3'