cDNA Clone HUNK

• Plasmid: pFBDual GST-TEV HUNK
• Parent Plasmid: pFastbacDual GST TEV2
• Expressed: GST-TEV HUNK
• Genbank: NM_014586 with silent changes to kill BamH1 sites and t1413c (silent) and t1548c (silent)
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCGGCGGCGGCGGGGGACGGGCTCCTGGGGGAGCCGGCGGCGCCTGGGGGCGGCGGCGGCGCGGAGGACGCGGCCAGGCCCGCGGCGGCCTGCGAGGGAAGTTTCCTGCCTGCCTGGGTGAGCGGCGTGCCCCGCGAGCGGCTCCGCGACTTCCAGCACCACAAGCGCGTGGGCAACTACCTCATCGGCAGCAGGAAGCTGGGCGAGGGCTCCTTTGCCAAGGTGCGCGAGGGGCTGCACGTGCTGACCGGGGAGAAGGTGGCCATAAAAGTCATTGATAAGAAGAGAGCCAAAAAGGACACCTATGTCACCAAAAACCTGCGGCGAGAGGGTCAGATCCAGCAGATGATCCGCCACCCCAATATCACTCAGCTCCTTGATATTTTAGAAACGGAAAACAGCTACTACCTGGTCATGGAGCTGTGCCCTGGGGGCAACCTGATGCACAAGATCTATGAGAAGAAGCGGCTGGAGGAGTCCGAAGCCCGCAGATACATCCGACAGCTCATCTCTGCCGTAGAGCACCTGCACCGGGCCGGGGTGGTCCACAGAGACTTGAAGATAGAGAATTTGCTACTAGATGAAGACAATAATATCAAGCTGATTGACTTTGGTTTGAGCAACTGCGCAGGCATCCTGGGTTACAGCGATCCGTTCAGCACACAGTGTGGCAGCCCTGCCTACGCTGCACCTGAACTGCTCGCCAGGAAGAAATACGGCCCCAAAATCGATGTCTGGTCCATAGGTGTGAACATGTATGCCATGTTGACCGGGACGCTGCCTTTCACGGTGGAGCCTTTCAGCCTGAGGGCTTTGTACCAGAAGATGGTAGACAAAGAAATGAACCCCCTCCCCACTCAGCTCTCCACAGGTGCCATCAGTTTCCTGCGCTCTCTCCTGGAACCTGATCCTGTGAAGAGGCCAAATATTCAGCAGGCACTGGCGAATCGCTGGCTTAATGAGAATTACACGGGCAAAGTGCCCTGTAATGTCACCTATCCCAACAGGATTTCTCTGGAAGATCTGAGCCCGAGCGTCGTGCTGCACATGACCGAGAAGCTGGGTTACAAGAACAGCGACGTGATCAACACTGTGCTCTCCAACCGCGCCTGCCACATCCTGGCCATCTACTTCCTCTTAAACAAGAAACTGGAGCGCTATTTGTCAGGGAAATCTGACATCCAGGACAGCCTCTGCTACAAGACCCGGCTCTACCAGATAGAAAAGTACAGGGCCCCCAAGGAGTCCTATGAGGCCTCTCTGGACACCTGGACACGAGATCTTGAATTCCATGCCGTGCAGGATAAAAAGCCCAAAGAACAAGAAAAAAGAGGGGATTTTCTTCATCGACCATTCTCCAAGAAGTTGGACAAGAACCTGCCCTCGCACAAACAGCCCTCAGGCTCGCTCATGACACAGATTCAGAACACCAAAGCCCTCCTGAAGGACCGGAAGGCCTCCAAGTCCAGCTTCCCCGACAAAGATTCCTTTGGCTGCCGCAATATTTTCCGCAAAACCTCAGATTCCAATTGTGTGGCTTCTTCCTCCATGGAGTTCATCCCCGTGCCACCGCCCAGGACCCCGAGGATTGTGAAGAAACCGGAGCCCCATCAGCCAGGGCCCGGAAGCACTGGCATCCCCCACAAGGAAGACCCCCTGATGCTGGACATGGTGCGCTCCTTCGAGTCTGTGGATCGCGACGACCACGTAGAAGTGCTGTCTCCCTCTCATCACTACAGGATTCTGAACTCCCCGGTCAGCTTGGCTCGCAGAAATTCCAGCGAGAGGACGCTGTCCCCGGGTCTGCCATCCGGAAGCATGTCGCCTCTCCATACTCCTTTGCATCCAACTCTGGTCTCTTTTGCTCACGAAGATAAGAACAGCCCCCCAAAAGAGGAGGGCCTGTGTTGCCCACCTCCGGTTCCCAGCAATGGCCCCATGCAGCCTCTGGGGAGCCCCAATTGTGTGAAAAGCCGAGGCCGGTTCCCTATGATGGGCATCGGACAGATGTTAAGGAAGCGCCATCAGAGTCTGCAGCCATCTGCAGATAGGCCCCTGGAGGCCAGCCTGCCCCCACTGCAGCCCCTAGCCCCTGTGAACCTTGCCTTTGACATGGCCGATGGGGTCAAGACCCAGTGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSMPAAAGDGLLGEPAAPGGGGG
AEDAARPAAACEGSFLPAWVSGVPRERLRDFQHHKRVGNYLIGSRKLGEG
SFAKVREGLHVLTGEKVAIKVIDKKRAKKDTYVTKNLRREGQIQQMIRHP
NITQLLDILETENSYYLVMELCPGGNLMHKIYEKKRLEESEARRYIRQLI
SAVEHLHRAGVVHRDLKIENLLLDEDNNIKLIDFGLSNCAGILGYSDPFS
TQCGSPAYAAPELLARKKYGPKIDVWSIGVNMYAMLTGTLPFTVEPFSLR
ALYQKMVDKEMNPLPTQLSTGAISFLRSLLEPDPVKRPNIQQALANRWLN
ENYTGKVPCNVTYPNRISLEDLSPSVVLHMTEKLGYKNSDVINTVLSNRA
CHILAIYFLLNKKLERYLSGKSDIQDSLCYKTRLYQIEKYRAPKESYEAS
LDTWTRDLEFHAVQDKKPKEQEKRGDFLHRPFSKKLDKNLPSHKQPSGSL
MTQIQNTKALLKDRKASKSSFPDKDSFGCRNIFRKTSDSNCVASSSMEFI
PVPPPRTPRIVKKPEPHQPGPGSTGIPHKEDPLMLDMVRSFESVDRDDHV
EVLSPSHHYRILNSPVSLARRNSSERTLSPGLPSGSMSPLHTPLHPTLVS
FAHEDKNSPPKEEGLCCPPPVPSNGPMQPLGSPNCVKSRGRFPMMGIGQM
LRKRHQSLQPSADRPLEASLPPLQPLAPVNLAFDMADGVKTQC*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45616
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental is pFBDual GST-TEV (DU37377)