cDNA Clone
HUNK

Plasmid
pET15b 6His SUMO HUNK
Parent Plasmid
pET15b 6His SUMO1
Expressed
6His-SUMO HUNK
Genbank
NM_014586 with silent changes to kill BamH1 sites and t1413c (silent) and t1548c (silent)
Tag
6His
SUMO
Species
Human
Sequence of Insert
GGATCCATGCCGGCGGCGGCGGGGGACGGGCTCCTGGGGGAGCCGGCGGCGCCTGGGGGCGGCGGCGGCGCGGAGGACGCGGCCAGGCCCGCGGCGGCCTGCGAGGGAAGTTTCCTGCCTGCCTGGGTGAGCGGCGTGCCCCGCGAGCGGCTCCGCGACTTCCAGCACCACAAGCGCGTGGGCAACTACCTCATCGGCAGCAGGAAGCTGGGCGAGGGCTCCTTTGCCAAGGTGCGCGAGGGGCTGCACGTGCTGACCGGGGAGAAGGTGGCCATAAAAGTCATTGATAAGAAGAGAGCCAAAAAGGACACCTATGTCACCAAAAACCTGCGGCGAGAGGGTCAGATCCAGCAGATGATCCGCCACCCCAATATCACTCAGCTCCTTGATATTTTAGAAACGGAAAACAGCTACTACCTGGTCATGGAGCTGTGCCCTGGGGGCAACCTGATGCACAAGATCTATGAGAAGAAGCGGCTGGAGGAGTCCGAAGCCCGCAGATACATCCGACAGCTCATCTCTGCCGTAGAGCACCTGCACCGGGCCGGGGTGGTCCACAGAGACTTGAAGATAGAGAATTTGCTACTAGATGAAGACAATAATATCAAGCTGATTGACTTTGGTTTGAGCAACTGCGCAGGCATCCTGGGTTACAGCGATCCGTTCAGCACACAGTGTGGCAGCCCTGCCTACGCTGCACCTGAACTGCTCGCCAGGAAGAAATACGGCCCCAAAATCGATGTCTGGTCCATAGGTGTGAACATGTATGCCATGTTGACCGGGACGCTGCCTTTCACGGTGGAGCCTTTCAGCCTGAGGGCTTTGTACCAGAAGATGGTAGACAAAGAAATGAACCCCCTCCCCACTCAGCTCTCCACAGGTGCCATCAGTTTCCTGCGCTCTCTCCTGGAACCTGATCCTGTGAAGAGGCCAAATATTCAGCAGGCACTGGCGAATCGCTGGCTTAATGAGAATTACACGGGCAAAGTGCCCTGTAATGTCACCTATCCCAACAGGATTTCTCTGGAAGATCTGAGCCCGAGCGTCGTGCTGCACATGACCGAGAAGCTGGGTTACAAGAACAGCGACGTGATCAACACTGTGCTCTCCAACCGCGCCTGCCACATCCTGGCCATCTACTTCCTCTTAAACAAGAAACTGGAGCGCTATTTGTCAGGGAAATCTGACATCCAGGACAGCCTCTGCTACAAGACCCGGCTCTACCAGATAGAAAAGTACAGGGCCCCCAAGGAGTCCTATGAGGCCTCTCTGGACACCTGGACACGAGATCTTGAATTCCATGCCGTGCAGGATAAAAAGCCCAAAGAACAAGAAAAAAGAGGGGATTTTCTTCATCGACCATTCTCCAAGAAGTTGGACAAGAACCTGCCCTCGCACAAACAGCCCTCAGGCTCGCTCATGACACAGATTCAGAACACCAAAGCCCTCCTGAAGGACCGGAAGGCCTCCAAGTCCAGCTTCCCCGACAAAGATTCCTTTGGCTGCCGCAATATTTTCCGCAAAACCTCAGATTCCAATTGTGTGGCTTCTTCCTCCATGGAGTTCATCCCCGTGCCACCGCCCAGGACCCCGAGGATTGTGAAGAAACCGGAGCCCCATCAGCCAGGGCCCGGAAGCACTGGCATCCCCCACAAGGAAGACCCCCTGATGCTGGACATGGTGCGCTCCTTCGAGTCTGTGGATCGCGACGACCACGTAGAAGTGCTGTCTCCCTCTCATCACTACAGGATTCTGAACTCCCCGGTCAGCTTGGCTCGCAGAAATTCCAGCGAGAGGACGCTGTCCCCGGGTCTGCCATCCGGAAGCATGTCGCCTCTCCATACTCCTTTGCATCCAACTCTGGTCTCTTTTGCTCACGAAGATAAGAACAGCCCCCCAAAAGAGGAGGGCCTGTGTTGCCCACCTCCGGTTCCCAGCAATGGCCCCATGCAGCCTCTGGGGAGCCCCAATTGTGTGAAAAGCCGAGGCCGGTTCCCTATGATGGGCATCGGACAGATGTTAAGGAAGCGCCATCAGAGTCTGCAGCCATCTGCAGATAGGCCCCTGGAGGCCAGCCTGCCCCCACTGCAGCCCCTAGCCCCTGTGAACCTTGCCTTTGACATGGCCGATGGGGTCAAGACCCAGTGCTAAGCGGCCGC

Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSMPAAAGDGLLGEPAAPGGGGGAEDAARPAAACEGSFLPAWVSGVP
RERLRDFQHHKRVGNYLIGSRKLGEGSFAKVREGLHVLTGEKVAIKVIDK
KRAKKDTYVTKNLRREGQIQQMIRHPNITQLLDILETENSYYLVMELCPG
GNLMHKIYEKKRLEESEARRYIRQLISAVEHLHRAGVVHRDLKIENLLLD
EDNNIKLIDFGLSNCAGILGYSDPFSTQCGSPAYAAPELLARKKYGPKID
VWSIGVNMYAMLTGTLPFTVEPFSLRALYQKMVDKEMNPLPTQLSTGAIS
FLRSLLEPDPVKRPNIQQALANRWLNENYTGKVPCNVTYPNRISLEDLSP
SVVLHMTEKLGYKNSDVINTVLSNRACHILAIYFLLNKKLERYLSGKSDI
QDSLCYKTRLYQIEKYRAPKESYEASLDTWTRDLEFHAVQDKKPKEQEKR
GDFLHRPFSKKLDKNLPSHKQPSGSLMTQIQNTKALLKDRKASKSSFPDK
DSFGCRNIFRKTSDSNCVASSSMEFIPVPPPRTPRIVKKPEPHQPGPGST
GIPHKEDPLMLDMVRSFESVDRDDHVEVLSPSHHYRILNSPVSLARRNSS
ERTLSPGLPSGSMSPLHTPLHPTLVSFAHEDKNSPPKEEGLCCPPPVPSN
GPMQPLGSPNCVKSRGRFPMMGIGQMLRKRHQSLQPSADRPLEASLPPLQ
PLAPVNLAFDMADGVKTQC*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU45625
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Price
£125.00
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