cDNA Clone Human UBQLN2 ∆Pxx

• Plasmid: pGEX6P-1 HALO TEV UBQLN2 delta 477-540
• Parent Plasmid: pGEX6P1 Halo TEV
• Expressed: GST-HALO-UBQLN2 ∆Pxx
• Genbank: NM_013444.3
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

ggatccATGGCTGAGAATGGCGAGAGCAGCGGCCCCCCGCGCCCCTCCCG
CGGCCCTGCTGCGGCCCAAGGCTCGGCTGCTGCCCCGGCTGAGCCTAAAA
TCATCAAAGTCACGGTGAAGACTCCCAAAGAGAAAGAGGAGTTCGCGGTG
CCCGAGAACAGCTCGGTTCAGCAGTTTAAGGAAGCGATTTCGAAACGCTT
CAAATCCCAAACCGATCAGCTAGTGCTGATTTTTGCCGGAAAAATCTTAA
AAGATCAAGATACCTTGATCCAGCATGGCATCCATGATGGGCTGACTGTT
CACCTTGTCATCAAAAGCCAGAACCGACCTCAGGGCCAGTCCACGCAGCC
TAGCAATGCCGCGGGAACTAACACTACCTCGGCGTCGACTCCCAGGAGTA
ACTCCACACCTATTTCCACAAATAGCAACCCGTTTGGGTTGGGGAGCCTG
GGAGGACTTGCAGGCCTTAGCAGCCTGGGCTTGAGCTCGACCAACTTCTC
TGAGCTCCAGAGCCAGATGCAGCAGCAGCTTATGGCCAGCCCTGAGATGA
TGATCCAAATAATGGAAAATCCCTTTGTTCAGAGCATGCTTTCGAATCCC
GATCTGATGAGGCAGCTCATTATGGCTAATCCACAGATGCAGCAATTGAT
TCAGAGAAACCCAGAAATCAGTCACCTGCTCAACAACCCAGACATAATGA
GGCAGACACTCGAAATTGCCAGGAATCCAGCCATGATGCAAGAGATGATG
AGAAATCAAGACCTGGCTCTTAGCAATCTAGAAAGCATCCCAGGTGGCTA
TAATGCTTTACGGCGCATGTACACTGACATTCAAGAGCCGATGCTGAATG
CCGCACAAGAGCAGTTTGGGGGTAATCCATTTGCCTCCGTGGGGAGTAGT
TCCTCCTCTGGGGAAGGTACGCAGCCTTCCCGCACAGAAAATCGCGATCC
ACTACCCAATCCATGGGCACCACCGCCAGCTACCCAGAGTTCTGCAACTA
CCAGCACGACCACAAGCACTGGTAGTGGGTCTGGCAATAGTTCCAGCAAT
GCTACTGGGAACACCGTTGCTGCCGCTAATTATGTCGCCAGCATCTTTAG
TACCCCAGGCATGCAGAGCCTGCTGCAACAGATAACTGAAAACCCCCAGC
TGATTCAGAATATGCTGTCGGCGCCCTACATGAGAAGCATGATGCAGTCG
CTGAGCCAGAATCCAGATTTGGCTGCACAGATGATGCTGAATAGCCCGCT
GTTTACTGCAAATCCTCAGCTGCAGGAGCAGATGCGGCCACAGCTCCCAG
CCTTCCTGCAGCAGATGCAGAATCCAGACACACTATCAGCCATGTCAAAC
CCAAGAGCAATGCAGGCTTTAATGCAGATCCAGCAGGGGCTACAGACATT
AGCCACTGAAGCACCTGGCCTGATTCCGAGCTTCGCTGCACCTAGTGAAA
CCACGAGTCCTACATCAGAATCTGGACCCAACCAGCAGTTCATTCAGCAA
ATGGTGCAGGCCCTGGCTGGAGCAAATGCTCCACAGCTGCCGAATCCAGA
AGTCAGATTTCAGCAACAACTGGAACAGCTCAACGCAATGGGGTTCTTAA
ACCGTGAAGCAAACTTGCAGGCCCTAATAGCAACAGGAGGCGACATCAAT
GCAGCCATTGAAAGGCTGCTGGGCTCCCAGCCATCGTAAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGENLYFQGGSMAENGESSGPPRP
SRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISK
RFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQST
QPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTN
FSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQ
LIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPG
GYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENR
DPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASI
FSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNS
PLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQ
TLATEAPGLIPSFAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPN
PEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43904
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please subclone human UBQLN2 ∆Pxx (deletion of residues 477-540) to the pGEX6P-1-HALO-TEV vector.; Parental: 24005