cDNA Clone HOIP

• Synonyms: RNF31
• Plasmid: pGEXTEV HOIP codon opt
• Parent Plasmid: pGEX TEV
• Expressed: GST TEV HOIP
• Genbank: NP_060469.4
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGCCTGGTGAAGAAGAAGAACGCGCATTTCTGGTTGCACGTGA
AGAACTGGCAAGCGCACTGCGTCGTGATAGCGGTCAGGCATTTAGCCTGG
AACAGCTGCGTCCGCTGCTGGCAAGCAGCCTGCCTCTGGCAGCACGTTAT
CTGCAGCTGGATGCAGCACGTCTGGTTCGTTGTAATGCACATGGTGAACC
GCGTAATTATCTGAATACCCTGAGCACCGCACTGAACATTCTGGAAAAAT
ATGGTCGTAATCTGCTGAGTCCGCAGCGTCCGCGTTATTGGCGTGGTGTG
AAATTTAACAATCCGGTTTTTCGTAGCACCGTTGATGCAGTTCAGGGTGG
TCGTGATGTTCTGCGTCTGTATGGTTATACCGAAGAACAGCCGGATGGTC
TGAGCTTTCCGGAAGGTCAAGAGGAACCGGATGAACATCAGGTTGCCACC
GTTACCCTGGAAGTTCTGCTGCTGCGTACCGAACTGAGCCTGCTGCTGCA
GAATACCCATCCGCGTCAGCAGGCACTGGAACAACTGCTGGAAGATAAAG
TTGAAGATGATATGCTGCAGCTGAGCGAATTTGATCCTCTGCTGCGTGAA
ATTGCACCGGGTCCGCTGACCACCCCGAGCGTTCCGGGTAGTACTCCGGG
TCCGTGTTTTCTGTGTGGTAGCGCACCGGGTACACTGCATTGTCCGAGCT
GTAAACAGGCACTGTGTCCGGCATGTGATCACCTGTTTCATGGTCATCCG
AGCCGTGCACATCATCTGCGTCAGACCCTGCCTGGTGTTCTGCAGGGCAC
CCATCTGAGCCCGAGCCTGCCTGCAAGTGCACAGCCTCGTCCGCAGAGCA
CCAGCCTGCTGGCACTGGGTGATAGCAGCCTGAGCAGCCCGAATCCGGCA
TCAGCACATCTGCCGTGGCATTGTGCAGCCTGTGCAATGCTGAATGAACC
GTGGGCAGTTCTGTGTGTTGCCTGTGATCGTCCGCGTGGTTGTAAAGGTC
TGGGTCTGGGCACCGAAGGTCCGCAGGGTACAGGTGGTCTGGAACCTGAT
CTGGCACGTGGTCGTTGGGCATGTCAGAGCTGTACCTTTGAAAATGAAGC
AGCAGCCGTTCTGTGTAGCATTTGCGAACGTCCGCGTCTGGCCCAGCCTC
CGAGCCTGGTTGTTGATAGCCGTGATGCAGGTATTTGTCTGCAGCCACTG
CAGCAGGGTGATGCACTGCTGGCGAGCGCACAGAGCCAGGTTTGGTATTG
TATTCATTGTACCTTTTGCAATAGCAGTCCGGGTTGGGTTTGTGTTATGT
GTAATCGTACCAGCTCACCGATTCCAGCACAGCATGCACCGCGTCCGTAT
GCGAGCAGCCTGGAAAAAGGTCCGCCTAAACCGGGTCCTCCGCGTCGTCT
GAGTGCACCGCTGCCGAGCAGCTGTGGTGATCCGGAAAAACAGCGTCAGG
ATAAAATGCGTGAAGAAGGTCTGCAACTGGTTAGCATGATTCGTGAAGGT
GAAGCAGCCGGTGCATGTCCGGAAGAAATCTTTAGTGCACTGCAGTATAG
CGGTACGGAAGTTCCTCTGCAATGGCTGCGTAGTGAACTGCCGTATGTTC
TGGAAATGGTTGCCGAACTGGCAGGTCAGCAAGATCCGGGTCTGGGTGCA
TTTAGCTGTCAAGAGGCACGTCGTGCATGGCTGGATCGTCATGGTAATCT
GGATGAAGCAGTTGAAGAATGTGTTCGTACCCGTCGTCGTAAAGTTCAAG
AACTGCAGAGCCTGGGTTTTGGTCCAGAAGAAGGTAGTCTGCAGGCCCTG
TTTCAGCATGGTGGTGATGTTAGCCGTGCCCTGACCGAACTGCAGCGCCA
GCGCCTGGAACCGTTTCGTCAGCGTCTGTGGGATAGTGGTCCGGAACCGA
CCCCGAGCTGGGATGGTCCGGATAAACAGTCACTGGTTCGTCGTCTGCTG
GCAGTTTATGCACTGCCGAGTTGGGGTCGTGCGGAACTGGCACTGTCACT
GCTGCAAGAAACCCCTCGTAATTATGAACTGGGAGATGTTGTTGAAGCGG
TTCGTCATAGCCAGGATCGTGCCTTTCTGCGTCGCCTGCTGGCCCAAGAA
TGTGCAGTTTGTGGTTGGGCACTGCCGCATAATCGTATGCAGGCGCTGAC
CAGCTGTGAATGTACCATTTGTCCGGATTGCTTTCGCCAGCATTTTACCA
TTGCACTGAAAGAAAAACACATCACCGATATGGTTTGTCCTGCATGTGGT
CGTCCGGATCTGACCGATGATACCCAGCTGCTGAGCTATTTTAGCACCCT
GGATATTCAGCTGCGCGAATCACTGGAACCAGATGCATATGCGCTGTTTC
ATAAAAAACTGACCGAAGGTGTTCTGATGCGTGATCCGAAATTTCTGTGG
TGTGCACAGTGTAGCTTTGGCTTTATCTATGAACGTGAACAGCTGGAAGC
AACCTGTCCGCAGTGTCATCAGACCTTTTGTGTTCGTTGCAAACGTCAGT
GGGAGGAACAGCATCGTGGTCGTAGCTGTGAAGATTTTCAGAATTGGAAA
CGTATGAACGATCCGGAATATCAGGCACAGGGTCTGGCAATGTATCTGCA
AGAGAATGGTATTGATTGCCCGAAATGCAAATTTAGCTATGCACTGGCAC
GCGGTGGTTGTATGCATTTTCATTGCACCCAGTGTCGTCATCAGTTTTGT
AGCGGTTGTTATAATGCCTTCTATGCCAAAAACAAATGCCCAGAACCGAA
TTGCCGTGTGAAAAAAAGCCTGCATGGCCATCATCCGCGTGATTGTCTGT
TTTATCTGCGTGATTGGACCGCACTGCGCCTGCAAAAACTGCTGCAGGAT
AATAATGTGATGTTTAATACCGAACCGCCTGCCGGTGCACGTGCGGTTCC
GGGTGGTGGTTGTCGTGTTATTGAACAGAAAGAAGTTCCGAACGGCCTGC
GTGATGAAGCATGTGGTAAAGAAACTCCGGCAGGTTATGCAGGTCTGTGT
CAGGCACATTATAAAGAATATCTGGTGAGCCTGATTAATGCCCATAGCCT
GGACCCTGCAACCCTGTATGAAGTTGAGGAACTGGAAACCGCAACCGAAC
GCTATCTGCATGTGCGTCCGCAGCCGCTGGCAGGCGAAGATCCTCCTGCA
TACCAGGCACGTCTGCTGCAGAAACTGACAGAAGAAGTGCCGCTGGGTCA
GAGCATTCCGCGTCGCCGTAAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSMPGEEEERAFLVAREELASAL
RRDSGQAFSLEQLRPLLASSLPLAARYLQLDAARLVRCNAHGEPRNYLNT
LSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRL
YGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQ
QALEQLLEDKVEDDMLQLSEFDPLLREIAPGPLTTPSVPGSTPGPCFLCG
SAPGTLHCPSCKQALCPACDHLFHGHPSRAHHLRQTLPGVLQGTHLSPSL
PASAQPRPQSTSLLALGDSSLSSPNPASAHLPWHCAACAMLNEPWAVLCV
ACDRPRGCKGLGLGTEGPQGTGGLEPDLARGRWACQSCTFENEAAAVLCS
ICERPRLAQPPSLVVDSRDAGICLQPLQQGDALLASAQSQVWYCIHCTFC
NSSPGWVCVMCNRTSSPIPAQHAPRPYASSLEKGPPKPGPPRRLSAPLPS
SCGDPEKQRQDKMREEGLQLVSMIREGEAAGACPEEIFSALQYSGTEVPL
QWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARRAWLDRHGNLDEAVEE
CVRTRRRKVQELQSLGFGPEEGSLQALFQHGGDVSRALTELQRQRLEPFR
QRLWDSGPEPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPR
NYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPHNRMQALTSCECTI
CPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRE
SLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCH
QTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMYLQENGIDC
PKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNCRVKKS
LHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRV
IEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLY
EVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRR
K*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43825
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: DNA insert is codon optimized for E coli expression