cDNA Clone KSR2

• Plasmid: pFBDualGST KSR2 + MKK1 untagged
• Parent Plasmid: pFastbacDual 6P3
• Expressed: GST KSR2
• Genbank: Q6VAB6.2
• Tag: GST
• Species: Human

Sequence of Insert

gtcgacATGGACGAGGAGAACATGACCAAGTCCGAAGAGcagcagcctct
gagtttgcaaaaagccttacagcagtgcgaactggtccaaaacatgatag
acttgagcatctccaacctggaagggcttaggaccaaatgtgctacctcc
aacgacctcacacaaaaagaaatccggaccctggagagcaagctggtgaa
gtacttcagccggcagctgtcctgcaaaaagaaggtagccttgcaggagc
gcaacgcggagctggacggcttcccccagctacggcactggttccgaatc
gtcgatgtgcgcaaggaggtcctggaggaaatctcccccggccagctgag
cctggaggacctcttggagatgacggatgaacaggtgtgcgagactgtgg
agaaatacggagccaaccgggaggagtgtgcccgcctcaacgcctccctc
tcctgcctcaggaatgtccacatgtcaggaggcaacctttccaaacaaga
ctggaccatccagtggcccacgacagagacggggaaggagaacaatcccg
tgtgccccccggagcccaccccgtggatccgcacccatctctcccagagc
cccagggtcccgtccaagtgcgtccagcactattgtcacaccagccccac
tcccggggcccCTGTGTACACCCACGTGGACAGGCTTACCGTGGACGCCT
ACCCGGGCTTGTGCCCGCCCCCGCCACTGGAGTCGGGCCACCGTTCCCTG
CCCCCATCGCCCCGGCAGCGGCACGCGGTCCGCACCCCGCCGCGCACCCC
CAACATCGTCACCACCGTGACCCCGCCGGGCACGCCGCCCATGAGGAAGA
AGAACAAGCTGAAGCCCCCGGGGACCCCACCGCCCTCCTCCCGAAAACTG
ATACACTTGATCCCGGGATTCACCGCGCTGCATCGGAGCAAATCCCACGA
GTTCCAGCTGGGGCACCGCGTGGACGAGGCCCACACGCCCAAAGCCAAGA
AGAAGAGCAAACCCTTGAACCTCAAGATCCACAGCAGCGTAGGCAGCTGC
GAGAACATCCCCTCTCAGCAGCGCTCCCCGCTGCTGTCCGAGCGCTCCCT
CCGCTCCTTCTTTGTGGGACACGCACCTTTCCTGCCTTCCACCCCTCCTG
TTCACACTGAGGCCAACTTCTCTGCAAACACACTGTCAGTGCCACGCTGG
TCCCCGCAGATCCCTCGCAGAGATCTTGGCAACTCCATCAAGCACAGGTT
TTCCACCAAGTACTGGATGTCTCAGACGTGCACAGTCTGTGGGAAAGGGA
TGCTTTTTGGCCTCAAGTGTAAAAACTGCAAGTTAAAGTGCCACAACAAA
TGCACCAAAGAAGCCCCACCCTGTCATCTTCTGATCATCCACCGAGGAGA
TCCAGCAAGGTTAGTCCGGACAGAGTCCGTTCCGTGTGACATCAACAACC
CTCTACGGAAGCCACCTCGCTATTCAGACCTGCACATCAGTCAGACGCTC
CCCAAAACCAACAAAATCAACAAGGACCACATCCCTGTCCCTTACCAGCC
AGACTCCAGCAGCAACCCCTCCTCCACGACGTCCTCCACGCCCTCCTCGC
CAGCACCCCCCCTCCCTCCTAGTGCCACGCCGCCTTCTCCCCTACACCCT
TCCCCACAGTGCACACGGCAGCAGAAGAACTTCAACCTGCCAGCATCCCA
CTACTACAAATACAAGCAGCAGTTCATCTTCCCAGATGTGGTGCCGGTGC
CGGAGACGCCGACCCGGGCGCCCCAGGTCATCCTGCATCCGGTGACCTCG
AATCCAATCTTGGAAGGAAATCCATTACTTCAAATTGAAGTGGAGCCAAC
GTCGGAGAATGAAGAGGTCCATGATGAGGCCGAAGAGTCAGAGGATGACT
TCGAGGAGATGAACCTGTCCCTCCTCTCGGCCCGGAGCTTCCCACGCAAG
GCCAGCCAGACCAGCATCTTCCTTCAGGAGTGGGACATCCCCTTTGAGCA
GCTGGAGATCGGCGAGCTCATTGGAAAGGGCCGCTTTGGGCAAGTGTACC
ACGGCCGCTGGCATGGCGAGGTGGCCATCCGGCTGATTGACATTGAGAGG
GACAACGAGGACCAGCTCAAGGCCTTCAAGCGGGAGGTGATGGCCTACAG
GCAGACACGGCATGAGAACGTGGTGCTTTTCATGGGTGCCTGCATGAGCC
CGCCTCACCTGGCCATCATCACCAGCCTCTGTAAGGGACGGACGCTCTAT
TCCGTTGTGAGGGATGCCAAAATCGTTTTGGATGTCAACAAAACCAGGCA
GATTGCTCAAGAAATTGTGAAGGGCATGGGCTACCTCCACGCCAAGGGAA
TCCTACACAAGGACCTCAAGTCAAAGAACGTCTTCTATGACAACGGCAAA
GTGGTCATCACGGACTTTGGACTCTTCAGCATTTCTGGGGTGCTGCAGGC
TGGCAGGCGGGAGGACAAACTGCGCATCCAGAATGGCTGGCTATGCCACC
TGGCACCAGAGATCATCCGCCAGCTGTCCCCCGACACAGAGGAGGATAAG
CTCCCCTTCTCCAAGCACTCTGACGTCTTTGCCCTTGGCACAATCTGGTA
TGAACTCCACGCCAGGGAATGGCCTTTCAAGACCCAACCAGCAGAGGCAA
TAATCTGGCAAATGGGCACAGGCATGAAACCCAACCTCAGCCAGATTGGC
ATGGGAAAAGAAATCTCGGACATTCTTCTCTTCTGCTGGGCCTTTGAACA
AGAAGAGAGACCTACCTTCACCAAGCTCATGGACATGCTGGAGAAACTGC
CAAAGCGAAACCGTCGCCTGTCTCACCCTGGACATTTCTGGAAGTCTGCA
GAGCTGTGAGCGGCCGCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMDEENMTKSEEQQ
PLSLQKALQQCELVQNMIDLSISNLEGLRTKCATSNDLTQKEIRTLESKL
VKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPGQ
LSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSK
QDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTS
PTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPR
TPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKS
HEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSER
SLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKH
RFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHR
GDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPY
QPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPA
SHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVE
PTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPF
EQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMA
YRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKT
RQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVL
QAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTI
WYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAF
EQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL*

• Antibiotic: Amp
• DU Number: DU43927
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee