cDNA Clone KSR2

• Plasmid: pFBHTc KSR2
• Parent Plasmid: pFastbac HTc
• Expressed: 6HIS TEV KSR2
• Genbank: Q6VAB6.2
• Tag: 6His
• Species: Human

Sequence of Insert

gtcgacATGGACGAGGAGAACATGACCAAGTCCGAAGAGcagca
gcctctgagtttgcaaaaagccttacagcagtgcgaactggtccaaaaca
tgatagacttgagcatctccaacctggaagggcttaggaccaaatgtgct
acctccaacgacctcacacaaaaagaaatccggaccctggagagcaagct
ggtgaagtacttcagccggcagctgtcctgcaaaaagaaggtagccttgc
aggagcgcaacgcggagctggacggcttcccccagctacggcactggttc
cgaatcgtcgatgtgcgcaaggaggtcctggaggaaatctcccccggcca
gctgagcctggaggacctcttggagatgacggatgaacaggtgtgcgaga
ctgtggagaaatacggagccaaccgggaggagtgtgcccgcctcaacgcc
tccctctcctgcctcaggaatgtccacatgtcaggaggcaacctttccaa
acaagactggaccatccagtggcccacgacagagacggggaaggagaaca
atcccgtgtgccccccggagcccaccccgtggatccgcacccatctctcc
cagagccccagggtcccgtccaagtgcgtccagcactattgtcacaccag
ccccactcccggggcccCTGTGTACACCCACGTGGACAGGCTTACCGTGG
ACGCCTACCCGGGCTTGTGCCCGCCCCCGCCACTGGAGTCGGGCCACCGT
TCCCTGCCCCCATCGCCCCGGCAGCGGCACGCGGTCCGCACCCCGCCGCG
CACCCCCAACATCGTCACCACCGTGACCCCGCCGGGCACGCCGCCCATGA
GGAAGAAGAACAAGCTGAAGCCCCCGGGGACCCCACCGCCCTCCTCCCGA
AAACTGATACACTTGATCCCGGGATTCACCGCGCTGCATCGGAGCAAATC
CCACGAGTTCCAGCTGGGGCACCGCGTGGACGAGGCCCACACGCCCAAAG
CCAAGAAGAAGAGCAAACCCTTGAACCTCAAGATCCACAGCAGCGTAGGC
AGCTGCGAGAACATCCCCTCTCAGCAGCGCTCCCCGCTGCTGTCCGAGCG
CTCCCTCCGCTCCTTCTTTGTGGGACACGCACCTTTCCTGCCTTCCACCC
CTCCTGTTCACACTGAGGCCAACTTCTCTGCAAACACACTGTCAGTGCCA
CGCTGGTCCCCGCAGATCCCTCGCAGAGATCTTGGCAACTCCATCAAGCA
CAGGTTTTCCACCAAGTACTGGATGTCTCAGACGTGCACAGTCTGTGGGA
AAGGGATGCTTTTTGGCCTCAAGTGTAAAAACTGCAAGTTAAAGTGCCAC
AACAAATGCACCAAAGAAGCCCCACCCTGTCATCTTCTGATCATCCACCG
AGGAGATCCAGCAAGGTTAGTCCGGACAGAGTCCGTTCCGTGTGACATCA
ACAACCCTCTACGGAAGCCACCTCGCTATTCAGACCTGCACATCAGTCAG
ACGCTCCCCAAAACCAACAAAATCAACAAGGACCACATCCCTGTCCCTTA
CCAGCCAGACTCCAGCAGCAACCCCTCCTCCACGACGTCCTCCACGCCCT
CCTCGCCAGCACCCCCCCTCCCTCCTAGTGCCACGCCGCCTTCTCCCCTA
CACCCTTCCCCACAGTGCACACGGCAGCAGAAGAACTTCAACCTGCCAGC
ATCCCACTACTACAAATACAAGCAGCAGTTCATCTTCCCAGATGTGGTGC
CGGTGCCGGAGACGCCGACCCGGGCGCCCCAGGTCATCCTGCATCCGGTG
ACCTCGAATCCAATCTTGGAAGGAAATCCATTACTTCAAATTGAAGTGGA
GCCAACGTCGGAGAATGAAGAGGTCCATGATGAGGCCGAAGAGTCAGAGG
ATGACTTCGAGGAGATGAACCTGTCCCTCCTCTCGGCCCGGAGCTTCCCA
CGCAAGGCCAGCCAGACCAGCATCTTCCTTCAGGAGTGGGACATCCCCTT
TGAGCAGCTGGAGATCGGCGAGCTCATTGGAAAGGGCCGCTTTGGGCAAG
TGTACCACGGCCGCTGGCATGGCGAGGTGGCCATCCGGCTGATTGACATT
GAGAGGGACAACGAGGACCAGCTCAAGGCCTTCAAGCGGGAGGTGATGGC
CTACAGGCAGACACGGCATGAGAACGTGGTGCTTTTCATGGGTGCCTGCA
TGAGCCCGCCTCACCTGGCCATCATCACCAGCCTCTGTAAGGGACGGACG
CTCTATTCCGTTGTGAGGGATGCCAAAATCGTTTTGGATGTCAACAAAAC
CAGGCAGATTGCTCAAGAAATTGTGAAGGGCATGGGCTACCTCCACGCCA
AGGGAATCCTACACAAGGACCTCAAGTCAAAGAACGTCTTCTATGACAAC
GGCAAAGTGGTCATCACGGACTTTGGACTCTTCAGCATTTCTGGGGTGCT
GCAGGCTGGCAGGCGGGAGGACAAACTGCGCATCCAGAATGGCTGGCTAT
GCCACCTGGCACCAGAGATCATCCGCCAGCTGTCCCCCGACACAGAGGAG
GATAAGCTCCCCTTCTCCAAGCACTCTGACGTCTTTGCCCTTGGCACAAT
CTGGTATGAACTCCACGCCAGGGAATGGCCTTTCAAGACCCAACCAGCAG
AGGCAATAATCTGGCAAATGGGCACAGGCATGAAACCCAACCTCAGCCAG
ATTGGCATGGGAAAAGAAATCTCGGACATTCTTCTCTTCTGCTGGGCCTT
TGAACAAGAAGAGAGACCTACCTTCACCAAGCTCATGGACATGCTGGAGA
AACTGCCAAAGCGAAACCGTCGCCTGTCTCACCCTGGACATTTCTGGAAG
TCTGCAGAGCTGTGAGCGGCCGCG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDMDEENMTKSEEQQP
LSLQKALQQCELVQNMIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLV
KYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPGQL
SLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQ
DWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSP
TPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRT
PNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSH
EFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERS
LRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHR
FSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRG
DPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQ
PDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPAS
HYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEP
TSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFE
QLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAY
RQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTR
QIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQ
AGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIW
YELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFE
QEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU43928
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee