cDNA Clone cyclin T1 CDK9

• Synonyms: CCNT1
• Plasmid: pFBdual6P-1 Cyclin T1 + CDK9
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GSt Cyclin T1
• Genbank: NM_001240.2 GI:17978465 with silent changes t453c, c1341g, c2034t
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAGGGAGAGAGGAAGAACAACAACAAACGGTGGTATTTCACTCGAGAACAGCTGGAAAATAGCCCATCCCGTCGTTTTGGCGTGGACCCAGATAAAGAACTTTCTTATCGCCAGCAGGCGGCCAATCTGCTTCAGGACATGGGGCAGCGTCTTAACGTCTCACAATTGACTATCAACACTGCTATAGTATACATGCATCGATTCTACATGATTCAGTCCTTCACACAGTTCCCTGGAAATTCTGTGGCTCCAGCAGCCTTGTTTCTAGCAGCTAAAGTGGAGGAGCAGCCCAAAAAATTGGAACATGTCATCAAGGTAGCACATACTTGTCTCCATCCTCAGGAATCCCTTCCTGATACTAGAAGTGAGGCTTATTTGCAACAAGTTCAAGATCTGGTCATTTTAGAAAGCATAATTTTGCAGACTTTAGGCTTTGAACTAACAATTGACCACCCACATACTCATGTAGTAAAGTGCACTCAACTTGTTCGAGCAAGCAAGGACTTAGCACAGACTTCTTACTTCATGGCAACCAACAGCCTGCATTTGACCACATTTAGCCTGCAGTACACACCTCCTGTGGTGGCCTGTGTCTGCATTCACCTGGCTTGCAAGTGGTCCAATTGGGAGATCCCAGTCTCAACTGACGGGAAGCACTGGTGGGAGTATGTTGACGCCACTGTGACCTTGGAACTTTTAGATGAACTGACACATGAGTTTCTACAGATTTTGGAGAAAACTCCCAACAGGCTCAAACGCATTTGGAATTGGAGGGCATGCGAGGCTGCCAAGAAAACAAAAGCAGATGACCGAGGAACAGATGAAAAGACTTCAGAGCAGACAATCCTCAATATGATTTCCCAGAGCTCTTCAGACACAACCATTGCAGGTTTAATGAGCATGTCAACTTCTACCACAAGTGCAGTGCCTTCCCTGCCAGTCTCCGAAGAGTCATCCAGCAACTTAACCAGTGTGGAGATGTTGCCGGGCAAGCGTTGGCTGTCCTCCCAACCTTCTTTCAAACTAGAACCTACTCAGGGTCATCGGACTAGTGAGAATTTAGCACTTACAGGAGTTGATCATTCCTTACCACAGGATGGTTCAAATGCATTTATTTCCCAGAAGCAGAATAGTAAGAGTGTGCCATCAGCTAAAGTGTCACTGAAAGAATACCGCGCGAAGCATGCAGAAGAATTGGCTGCCCAGAAGAGGCAACTGGAGAACATGGAAGCCAATGTGAAGTCACAATATGCATATGCTGCCCAGAATCTCCTTTCTCATCATGATAGCCATTCTTCAGTCATTCTAAAAATGCCCATAGAGGGTTCAGAAAACCCGGAGCGGCCTTTTCTGGAAAAGGCTGACAAAACAGCTCTCAAAATGAGAATCCCAGTGGCAGGTGGAGATAAAGCTGCGTCTTCAAAACCAGAGGAGATAAAAATGCGCATAAAAGTCCATGCTGCAGCTGATAAGCACAATTCTGTAGAGGACAGTGTTACAAAGAGCCGAGAGCACAAAGAAAAGCACAAGACTCACCCATCTAATCATCATCATCATCATAATCACCACTCACACAAGCACTCTCATTCCCAACTTCCAGTTGGTACTGGGAACAAACGTCCTGGTGATCCAAAACATAGTAGCCAGACAAGCAACTTAGCACATAAAACCTATAGCTTGTCTAGTTCTTTTTCCTCTTCCAGTTCTACTCGTAAAAGGGGACCCTCTGAAGAGACTGGAGGGGCTGTGTTTGATCATCCAGCCAAGATTGCCAAGAGTACTAAATCCTCTTCCCTAAATTTCTCCTTCCCTTCACTTCCTACAATGGGTCAGATGCCTGGGCATAGCTCAGACACAAGTGGCCTTTCCTTTTCACAGCCCAGCTGTAAAACTCGTGTCCCTCATTCGAAACTGGATAAAGGGCCCACTGGGGCCAATGGTCACAACACGACCCAGACAATAGACTATCAAGACACTGTGAATATGCTTCACTCCCTGCTCAGTGCCCAGGGTGTTCAGCCCACTCAGCCTACTGCATTTGAATTTGTTCGTCCTTATAGTGACTATCTGAATCCTCGGTCTGGTGGAATCTCCTCGAGATCTGGCAATACAGACAAACCCCGGCCACCACCTCTGCCATCAGAACCTCCTCCACCACTTCCACCCCTTCCTAAGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEGERKNNNKRWYFTREQL
ENSPSRRFGVDPDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRF
YMIQSFTQFPGNSVAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLP
DTRSEAYLQQVQDLVILESIILQTLGFELTIDHPHTHVVKCTQLVRASKD
LAQTSYFMATNSLHLTTFSLQYTPPVVACVCIHLACKWSNWEIPVSTDGK
HWWEYVDATVTLELLDELTHEFLQILEKTPNRLKRIWNWRACEAAKKTKA
DDRGTDEKTSEQTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEES
SSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGS
NAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYA
YAAQNLLSHHDSHSSVILKMPIEGSENPERPFLEKADKTALKMRIPVAGG
DKAASSKPEEIKMRIKVHAAADKHNSVEDSVTKSREHKEKHKTHPSNHHH
HHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQTSNLAHKTYSLSSSFSSSS
STRKRGPSEETGGAVFDHPAKIAKSTKSSSLNFSFPSLPTMGQMPGHSSD
TSGLSFSQPSCKTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLS
AQGVQPTQPTAFEFVRPYSDYLNPRSGGISSRSGNTDKPRPPPLPSEPPP
PLPPLPK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU43929
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Urgent cloning for AZ project