cDNA Clone OTUB1

• Plasmid: pcDNA frt to -Flag-OTUB1 sprot1+3
• Parent Plasmid: pcDNA5 FRT/TO FLAG1
• Expressed: Flag-OTUB1 sprot1+3
• Genbank: NP_060140.2
• Tag: FLAG

Sequence of Insert

ATGGCGGCGGAGGAACCTCAGCAGCAGAAGCAGGAGCCGCTGGGCAGCGACTCCGAAGGTGTTAACTGTCTGGCCTATGATGAAGCCATCATGGCTCAGCAGGACCGAATTCAGCAAGAGATTGCTGTGCAGAACCCTCTGGTGTCAGAGCGGCTGGAGCTCTCGGTCCTATACAAGGAGTATGCTGAAGATGACAACATCTATCAACAGAAGATCAAGGACCTCCACAAAAAGTACTCGTACATCCGCAAGACCAGGCCTGACGGCAACTGTTTCTATCGGGCTTTCGGATTCTCCCACTTGGAGGCACTGCTGGATGACAGCAAGGAGTTGCAGCGGTTCAAGGCTGTGTCTGCCAAGAGCAAGGAAGACCTGGTGTCCCAGGGCTTCACTGAATTCACAATTGAGGATTTCCACAACACGTTCATGGACCTGATTGAGCAGGTGGAGAAGCAGACCTCTGTCGCCGACCTGCTGGCCTCCTTCAATGACCAGAGCACCTCCGATTATTTAGTAGTCTACCTGCGGCTGCTCACCTCGGGCTACCTGCAGCGCGAGAGCAAATTCTTTGAACATTTCATCGAGGGTGGACGGACTGTCAAGGAGTTCTGCCAGCAGGAGGTGGAGCCCATGTGCAAGGAGAGCGACCACATCCACATCATTGCGCTGGCCCAGGCCCTCAGCGTGTCCATCCAGGTGGAGTACATGGACCGCGGCGAGGGCGGCACCACCAATCCGCACATCTTCCCTGAGGGCTCCGAGCCCAAGGTCTACCTTCTCTACCGGCCTGGACACTACGATATCCTCTACAAATAG

Amino Acid Sequence

MDYKDDDDKGSMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42801
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: pcDNA5-FRT/TO-Flag-OTUB1 both harbouring resistance to the shRNA and siRNA of OTUB1 (as previously done).