cDNA Clone CDK13

• Plasmid: pFBDualGST CDK13 + Cyclin K
• Parent Plasmid: pFastbacDual 6P1
• Expressed: GST-CDK13 + cyclin K (untagged)
• Genbank: Q14004.2
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCatgccgagcagctcggacacggcgctggggggaggcgggggcct gagctgggcggagaagaggttggaggaacgccgcaagcggaggcgattcc tgtcccctcagcagccgccgctgctgttgccgctcctgcagccgcagctc ctgcaaccgccgccgcccccgccgcctctgctcttcctggctgctcccgg cacggccgccgccgcagccgccgccgccgcggcctcctcctcttgcttca gcccgggcccccctctggaggtcaagcggctggcgagaggcaagaggcgc gcaggagggcggcagaagcggcgtcgcgggccccgcgccgggcaggaggc ggagaagcgtcgggtcttctcgctgccccagccgcagcaggacggcggtg gcggtgctagtagcggcgggggtgtgaccccgctggtggaatacgaggat gtgagctcccagtccgagcaggggctgctgctggggggggccagcgcggc aacggcggcgacggctgccgggggaacggggggcagcggcgggagtccgg cctcctcctccggcacccagcggcgcggggaggggtcggagcgcaggccc cgccgggaccgccgcagcagcagtggccgcagcaaagagcgccaccgcga gcaccggcggcgggatgggcagcgcggtggcagcgaggcctccaagtccc gcagccgccacagccacagcggcgaggaacgggccgaggtcgccaagagc ggcagcagcagcagcagcggcggTcgccggaaaagcgcttcggccacatc cagcagcagtagcagccgcaaggaccgggactcgaaggcccaccgcagcc ggactaagtcgtccaaggagccgccttcggcctacaaggaaccgcccaag gcctaccgggaggacaagaccgagcctaaggcctacaggcggcggcggtc cctcagcccactgggaggccgggacgacagcccggtgtcccacagggcct ctcagagcctgaggagccgcaagtcccccagcccggcaggaggtggcagc agcccctattctcggcggctgccgcgctccccgagcccctacagtcgccg ccgctcccccagctacagccgccacagctcctacgagcggggcggcgacg tgtcccctagtccctacagcagcagcagctggcgccgctctcgcagtccc tacagccctgtgctcagacggtctggaaaatcccgaagcagaagcccgta ttcatctaggcattcaagatctcgtagcaggcacagattgtctagatcca gaagtcgtcattctagtatttctcctagcacactaactctgaagagtagc ctggcagctgaattgaacaagaataaaaaagcacgagcagcagaggcagc aagagccgcagaagcagcgaaagctgcagaagcaactaaggctgctgagg ctgctgccaaggctgcaaaagcttcaaacacttctacacctaccaagggg aacacggaaactagtgccagtgcatcacaaacaaaccatgtgaaggatgt gaagaaaattaaaattgaacatgcaccttctccctcaagtggtggaactt taaaaaatgacaaagcaaaaacaaagccacctcttcaggtaacgaaggtg gaaaataatttgattgtagataaagccaccaagaaagcagtcatagttgg aaaggagagtaaatctgctgctacaaaggaggaatcagtatctcttaaag agaaaaccaaaccacttacaccaagcataggagccaaggagaaggagcaa catgtagctttagtcacctctacattaccaccgttacctttgcctcccat gctgcctgaagataaagaagctgatagcttacgaggaaatatttcagtaa aagcagttaaaaaagaagtagaaaagaaactccgatgtcttcttgctgat ttaccgctgccccctgagctaccaggaggagatgatctttcaaagagtcc agaggaaaagaaaacaacaacacagttacatagtaaaaggaggcctaaaa tatgtgggcctcgctatggtgaaaccaaagaaaaagatattgactgggga aaactctgcgtggataaatttgatatcatcggaattattggagaaggtac ttacggacaagtttacaaagccagggataaagacactggagaaatggtag ccttaaaaaaagtacgtctggataatgaaaaggaaggctttccaattaca gcaattcgagaaattaaaattctccggcagcttacccatcagagtattat caatatgaaggaaatagtgactgataaagaagatgctttggatttcaaga aggacaaaggtgcattttatctggtgtttgaatatatggaccatgatctg atgggactactggaatcaggcttggttcattttaatgaaaatcacataaa gtcatttatgagacagctcatggagggtctggattattgtcataagaaga actttttgcatagagatattaaatgttccaatatccttctaaataataga gggcagataaaacttgcagactttggacttgctcgattgtatagctcaga agaaagtcggccgtatactaacaaggtaattactttatggtaccgtccac ctgaactgctactgggagaagaacgatacacaccagccattgatgtatgg agctgtggctgtatccttggcgaactcttcactaaaaaacctatatttca agcaaatcaggaacttgcacaactagaattaataagccgaatatgtggga gtccatgtcctgcagtgtggcctgatgtaatcaaactaccatatttcaac accatgaaaccaaagaagcaatatcgtcgaaagttaagagaagaatttgt ttttattcctgcagctgcgctagacttatttgattacatgcttgccttgg aCcctagtaagcgctgcactgctgaacaggctcttcagtgcgagttcctc cgagatgtggaaccctcaaaaatgcctccaccagatctccctttatggca agattgtcatgagttatggagtaaaaagcgaagaagacagaagcagatgg gcatgactgatgatgtttccacaattaaagcccccaggaaggacttgtct ctgggcttggatgacagcagaaccaacacaccccagggtgtgctgccatc ttcacagctgaaatctcagggcagctcaaatgtggcacctgtaaaaacag gccctggacagcacttaaaccacagtgaattggcaattctactaaaccta ctacaatctaaaacaagtgttaatatggctgattttgtccaagtgttgaa cattaaggtaaactctgagactcaacagcagctaaataaaataaaccttc ctgctggaattttggcaacaggtgaaaaacagacagatccatcaacacca caacaggagtcttcgaaaccgttgggaggaattcagccttcttctcagac catccagcctaaaatggagactgatgctgcccaggcggctgtgcagagtg catttgcagttctgttgactcagttaataaaggctcagcagtcaaagcag aaagatgtgctactagaagagagggaaaatggatcgggacatgaagcgtc attacaactcaggccacctccagaacctagcactccggtgtcgggacaag atgacctcatccagcatcaagatatgaggatcttggagctaacgccagaa ccagaccggcctcgaattctgcctcctgaccaacgacctcccgagcctcc tgaaccaccaccagtcactgaggaagatctagattatcggacagaaaacc agcatgtacccaccaccagttcttcattaactgaccctcatgccggagtg aaggcagccctgttacagctgcttgctcagcatcagccccaggatgaccc caaaagagaaggtgggattgattatcaagcaggagacacttacgtgtcca cttcagactacaaggacaactttggTtcctcttctttctcttctgctcct tatgttagcaatgatggtctaggaagcagttctgctccaccactagaacg acgtagtttcattggaaattcagatattcagtctttggataactacagta ctgcttcatctcattctggtggtccacctcagccttctgccttttctgag tcatttcccagttcagtagctggatatggagacatttacctcaatgctgg tcccatgttgtttagtggagacaaggaccatagatttgaatatagccatg gtcctattgcagtcctggcaaacagcagtgacccttccacggggccagag agtactcatcctttgccagcaaagatgcacaactataactatggtggtaa cttacaggaaaatccgagtggccccagcctcatgcatggacagacctgga cttctcctgcccaaggacctggatattcacaaggatacaggggacatatt agcacatcaactggcagaggcagaggcagagggttaccatactgagcggc cgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMPSSSDTALGGGGGLSWAE KRLEERRKRRRFLSPQQPPLLLPLLQPQLLQPPPPPPPLLFLAAPGTAAA AAAAAAASSSCFSPGPPLEVKRLARGKRRAGGRQKRRRGPRAGQEAEKRR VFSLPQPQQDGGGGASSGGGVTPLVEYEDVSSQSEQGLLLGGASAATAAT AAGGTGGSGGSPASSSGTQRRGEGSERRPRRDRRSSSGRSKERHREHRRR DGQRGGSEASKSRSRHSHSGEERAEVAKSGSSSSSGGRRKSASATSSSSS SRKDRDSKAHRSRTKSSKEPPSAYKEPPKAYREDKTEPKAYRRRRSLSPL GGRDDSPVSHRASQSLRSRKSPSPAGGGSSPYSRRLPRSPSPYSRRRSPS YSRHSSYERGGDVSPSPYSSSSWRRSRSPYSPVLRRSGKSRSRSPYSSRH SRSRSRHRLSRSRSRHSSISPSTLTLKSSLAAELNKNKKARAAEAARAAE AAKAAEATKAAEAAAKAAKASNTSTPTKGNTETSASASQTNHVKDVKKIK IEHAPSPSSGGTLKNDKAKTKPPLQVTKVENNLIVDKATKKAVIVGKESK SAATKEESVSLKEKTKPLTPSIGAKEKEQHVALVTSTLPPLPLPPMLPED KEADSLRGNISVKAVKKEVEKKLRCLLADLPLPPELPGGDDLSKSPEEKK TTTQLHSKRRPKICGPRYGETKEKDIDWGKLCVDKFDIIGIIGEGTYGQV YKARDKDTGEMVALKKVRLDNEKEGFPITAIREIKILRQLTHQSIINMKE IVTDKEDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFNENHIKSFMR QLMEGLDYCHKKNFLHRDIKCSNILLNNRGQIKLADFGLARLYSSEESRP YTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANQE LAQLELISRICGSPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFVFIPA AALDLFDYMLALDPSKRCTAEQALQCEFLRDVEPSKMPPPDLPLWQDCHE LWSKKRRRQKQMGMTDDVSTIKAPRKDLSLGLDDSRTNTPQGVLPSSQLK SQGSSNVAPVKTGPGQHLNHSELAILLNLLQSKTSVNMADFVQVLNIKVN SETQQQLNKINLPAGILATGEKQTDPSTPQQESSKPLGGIQPSSQTIQPK METDAAQAAVQSAFAVLLTQLIKAQQSKQKDVLLEERENGSGHEASLQLR PPPEPSTPVSGQDDLIQHQDMRILELTPEPDRPRILPPDQRPPEPPEPPP VTEEDLDYRTENQHVPTTSSSLTDPHAGVKAALLQLLAQHQPQDDPKREG GIDYQAGDTYVSTSDYKDNFGSSSFSSAPYVSNDGLGSSSAPPLERRSFI GNSDIQSLDNYSTASSHSGGPPQPSAFSESFPSSVAGYGDIYLNAGPMLF SGDKDHRFEYSHGPIAVLANSSDPSTGPESTHPLPAKMHNYNYGGNLQEN PSGPSLMHGQTWTSPAQGPGYSQGYRGHISTSTGRGRGRGLPY*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU46224
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee