cDNA Clone HECTD1

• Plasmid: pcDNA5D FRT/TO FLAG Hectd1 G1739-N2610
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: Flag HECTD1 G1739-N2610
• Genbank: Uniprot: Q9ULT8, Genbank: CCDS41939.1
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccGGGAGCCGACCTATTGGGGAGCAGGAAGAAGAAGAGTACGAAACTAAAGGAGGACGCCGGAGAACATGGGATGATGATTATGTGCTAAAGAGACAGTTTTCTGCATTGGTTCCTGCTTTTGATCCTAGACCTGGTCGTACTAATGTCCAGCAGACAACTGATCTAGAAATACCACCCCCAGGGACCCCTCATTCAGAGCTCTTGGAAGAAGTCGAATGTACTCCGTCACCTCGATTAGCTCTCACTTTGAAAGTAACAGGTCTTGGAACGACTCGTGAAGTTGAATTACCACTCACCAATTTCAGATCAACCATCTTTTACTATGTACAAAAATTGCTTCAATTGTCCTGTAATGGCAATGTGAAATCAGATAAACTTAGGCGTATTTGGGAGCCCACATACACAATCATGTACAGAGAAATGAAGGATTCTGATAAAGAAAAGGAAAATGGAAAAATGGGTTGCTGGTCTATAGAGCATGTGGAGCAGTACCTTGGCACTGATGAATTACCAAAGAATGACTTGATAACCTACCTGCAGAAGAATGCAGACGCTGCTTTCCTGCGCCACTGGAAATTAACTGGCACTAATAAAAGTATTAGGAAAAACAGAAATTGTTCTCAGCTCATAGCTGCATATAAGGATTTTTGTGAGCATGGAACAAAGTCTGGGTTAAACCAGGGGGCCATTTCTACTCTTCAAAGTAGTGATATTCTTAATTTAACAAAAGAACAACCTCAGGCCAAAGCAGGCAATGGACAGAACTCTTGTGGAGTAGAAGATGTCCTTCAGCTTCTGCGTATTCTATATATAGTTGCAAGTGACCCTTATTCAAGAATATCCCAGGAAGATGGTGATGAACAGCTTCAGTTTACTTTTCCACCAGATGAATTCACTAGCAAAAAAATTACAACAAAAATATTACAGCAGATTGAGGAACCATTGGCACTGGCAAGTGGGGCTCTGCCAGACTGGTGTGAACAATTAACCAGCAAATGTCCTTTTCTAATACCATTTGAAACTAGACAGCTTTATTTCACATGTACAGCATTTGGCGCCTCAAGAGCAATAGTATGGTTACAGAACCGACGTGAAGCCACTGTGGAGCGAACGAGAACCACAAGCAGTGTTAGGCGAGATGACCCTGGAGAGTTTCGAGTTGGTCGTCTCAAGCATGAAAGAGTAAAAGTTCCACGTGGCGAGTCACTGATGGAATGGGCTGAGAATGTCATGCAAATACATGCAGATCGGAAATCAGTTCTTGAGGTTGAATTTTTAGGAGAAGAAGGAACTGGCTTGGGACCCACATTAGAGTTTTATGCTCTGGTGGCAGCAGAATTCCAGAGAACTGACTTGGGAGCTTGGCTTTGTGATGATAATTTTCCAGATGATGAATCTCGTCACGTTGATCTTGGAGGTGGATTGAAACCTCCTGGATATTATGTGCAGAGGTCATGTGGACTGTTCACAGCACCATTTCCACAGGATAGTGATGAGCTTGAAAGGATCACGAAACTGTTTCATTTCCTTGGAATTTTCTTGGCCAAATGCATTCAAGACAATAGACTTGTGGACTTACCTATTTCTAAACCTTTTTTTAAACTTATGTGTATGGGTGACATTAAAAGCAATATGAGTAAACTGATTTATGAGTCACGAGGTGATAGAGACTTACACTGTACTGAAAGTCAGTCTGAAGCTTCTACAGAAGAAGGTCATGATTCACTCTCGGTAGGAAGCTTTGAAGAGGATTCAAAATCAGAATTTATTCTTGATCCCCCTAAACCAAAACCCCCAGCTTGGTTTAATGGAATTTTGACTTGGGAAGACTTTGAATTAGTAAACCCACACAGAGCCAGATTTTTAAAAGAAATTAAAGACCTTGCTATCAAGAGGCGCCAAATTTTAAGCAACAAAGGTCTTTCTGAAGATGAGAAGAACACAAAATTACAGGAACTAGTGCTGAAGAATCCATCAGGTTCTGGGCCTCCACTTAGCATAGAGGATTTAGGTTTAAATTTCCAGTTTTGCCCTTCCTCAAGAATATATGGTTTTACAGCTGTGGATCTCAAGCCAAGTGGTGAAGATGAGATGATAACAATGGATAATGCAGAAGAATATGTGGATTTGATGTTTGACTTTTGTATGCATACGGGTATTCAGAAACAAATGGAAGCCTTTAGAGATGGGTTTAATAAAGTTTTTCCAATGGAGAAATTAAGTTCCTTCAGCCATGAAGAAGTCCAAATGATTCTTTGTGGAAACCAGTCACCATCCTGGGCAGCAGAGGATATTATCAATTACACTGAACCTAAGCTGGGTTATACACGTGACAGCCCTGGTTTCCTGAGGTTTGTGAGGGTTTTATGTGGCATGTCTTCTGATGAAAGGAAAGCATTCTTGCAGTTTACCACTGGTTGTTCAACTCTACCCCCAGGTGGACTGGCTAACCTGCATCCCAGGCTCACGGTTGTACGCAAGGTTGATGCTACTGATGCAAGCTATCCATCAGTCAATACATGTGTGCATTACCTTAAGTTGCCTGAATATTCTTCCGAGGAGATCATGAGAGAGCGCCTGCTAGCTGCTACAATGGAGAAAGGCTTTCATCTCAATTGAgcggccgc

Amino Acid Sequence

MDYKDDDDKGSGSRPIGEQEEEEYETKGGRRRTWDDDYVLKRQFSALVPAFDPRPGRTNVQQTTDLEIPPPGTPHSELLEEVECTPSPRLALTLKVTGLGTTREVELPLTNFRSTIFYYVQKLLQLSCNGNVKSDKLRRIWEPTYTIMYREMKDSDKEKENGKMGCWSIEHVEQYLGTDELPKNDLITYLQKNADAAFLRHWKLTGTNKSIRKNRNCSQLIAAYKDFCEHGTKSGLNQGAISTLQSSDILNLTKEQPQAKAGNGQNSCGVEDVLQLLRILYIVASDPYSRISQEDGDEQLQFTFPPDEFTSKKITTKILQQIEEPLALASGALPDWCEQLTSKCPFLIPFETRQLYFTCTAFGASRAIVWLQNRREATVERTRTTSSVRRDDPGEFRVGRLKHERVKVPRGESLMEWAENVMQIHADRKSVLEVEFLGEEGTGLGPTLEFYALVAAEFQRTDLGAWLCDDNFPDDESRHVDLGGGLKPPGYYVQRSCGLFTAPFPQDSDELERITKLFHFLGIFLAKCIQDNRLVDLPISKPFFKLMCMGDIKSNMSKLIYESRGDRDLHCTESQSEASTEEGHDSLSVGSFEEDSKSEFILDPPKPKPPAWFNGILTWEDFELVNPHRARFLKEIKDLAIKRRQILSNKGLSEDEKNTKLQELVLKNPSGSGPPLSIEDLGLNFQFCPSSRIYGFTAVDLKPSGEDEMITMDNAEEYVDLMFDFCMHTGIQKQMEAFRDGFNKVFPMEKLSSFSHEEVQMILCGNQSPSWAAEDIINYTEPKLGYTRDSPGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU25182
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental Plasmid DU41457