cDNA Clone KIAA2022

• Plasmid: pcDNA5 FRT/TO GFP-NLS KIAA2022 L281E V283E
• Parent Plasmid: pcDNA5 FRT/TO GFP NLS
• Expressed: GFP-NLS-KIAA2022 L281E V283E
• Genbank: NM_001008537.2 with silent changes to kill internal BamH1 sites
• Tag: GFP
• Tag: NLS
• Species: Human

Sequence of Insert

GGATCCATGGATAACCAACAAGATAAGGCTATTGTTGCCTCAGCCAACGGAGAAAACACTCTGATTAATGGGGTCAAAGAAAATGACTCAGAGGACCAGGATGTGGCAATGAAGTCATTTGCAGCTCTAGAAGCTGCTGCACCTATCCAGCCTACACCGGTGGCACAAAAAGAGACCCTGATGTATCCCAGAGGTCTCCTGCCTCTACCCTCTAAGAAGCCCTGTATGCAGAGCCCGCCCTCTCCTTTGGGCCTGATTGAAGCACCCGAACATGCTGCTAATAGTGCTTCTGTGAATGCCATCTCCCTCACATCTGGCATTGCAAAAGGCCTGAACACATGGTCACTTCCCAATGAATGTGAGAAAGCTCCATTTGCCATAATGGAGCCTGCAGGCATGTCAGCTCTGAATGGGGACTGTCTCATGCAGCCAAGTCGGACTTGCTTAGGCTGCTTCATGGAATCCAAGGATGCAGTAGATCCTGAGCCAGGGATCAGTCTGAAAGTTGGTGATCTAAATAGGGATTATGAAACGTGTGCAGTCTCTGATATAGGGATTCAGTGTATTAATGCTGGAGAAAATATGAAATATGGAGAGCAGCTGCTCTCAGACCAGCTCCTAGGCTTCCCCCTGCATAAGTCAAGGGCAGGAGACAGACGAGAAACTGAGAAACCTGACATTGACTTGGAGGACCCGGCTCAGAAAAGCTATTATGAGGCATTACTGTTAGACAAGTGCAATACAGAAGAAGCTTTGCTTGCAAATTCCAATCAGGATTGGGGTTACTTCGAGACTTTTATTAGTGAAAGTAAGATTGAACTGCTTGACCTCTGTTCCAAAAATGAGGAGTCTGAGAACCTATTCTCTGAAGAAGATGTGGATAACTACATGTTTGATGATGATGAATCAACACTAGGCAGTGATGTCTGCTCCCTGAAAATTCGATATGAATCCTTTCAGGACAATGTTCGAGACAAGACTACTCTTTTGATGCAGGAAGATGCCCAATTCAACTTTTTTCCCAGCGTCTTTACTACCTGCCCCAAGCGAGAGTCTAAGAGTGGGGCCCTGAAGCAGAGCAGTGATTTTTCCCAATTCAAGGTCCCTGATGTGAGCATCATCTGGGGGGAGGAAGATAAAAACTTGGACAAGAAGAAAGGCAAAGAGGAAGGACAGGAAGACAAAGGTGTAGAGAAGAAAGATGGAAAGGATAATGGAGAAAAGCCTGCCTTAAATAAACCATGCAGTGGGACTGAAGTAGAGCAACTTAAGAATCCAAAGCAGGGCCATCTTGCTAATTCCCTGGAGACATCAGGGAGTTTCAGTGATGATAGTTCCTTCATTGAGATCTCATATGATGCTATGGGTGAGATCAAGGACTGTAGTCGCTATATGGCTCGGGACACTAATTCTGGCAGCTCCTCCTCCCAACAGAACTATGGGCTGCGAGCCAAGAGAAAAGTCAGATACAGTGAAGATTATCTATATGATGTTGACTCACTAGAGGGTGAAAAAGTAAATGAGAGGAAGGAATGGCTGCCAGTTGGTTCCAAAGAGGAAGATGATGATGAATGGTGTCCCAAAAAGAGAAGAAAAGTAACCCGTAAGGAGCCCCCTGTTATTATCAAATATATCATCATTAATCGCTTTAAAGGTGAGAAGAACATGCTGGTGAAGTTGGGTAAGGTGGATGCCAGTGAGACAACAGTGAATTTGAGTGAGAATCAGCTCAACAAATATGCCAAGCTGGCACCCTTGAAAGGCTTCTGGCAAAAGAAGAAGAAGCAGAGAAACACCAACACGGACTCCATCAAGACACCTTTTTCCCAAAAGCAAAGCTTTGAACCAGGTAGCTTTGAGGTGTCATTTCTGCCACCTGCTCGCAAACGAAAATCTAAACTTGGCAACAGGCACAGGATTCAAAGAATCCCATCCATTGAAATTTCAGCAAGTAGTAAACAGATTTCATTATGTAATGATCAGAGGCACGCTAGTAATCATAAAGAAGATGGAGGCCTAAAAGGTACACTGAAGTCAGCACCTCTGGGTGCTCCTAGCTGTGCAAATGGATCACATTTAAATGACATCACAGGCCCTGACTCAGTGAAAGTCAAAGCCCAAGACACAGAGTTTAAGGGGCCAGAGAGGAAAGTGCTCAATAAAATCAAATTTAAAAGTGAAGCTAGGTTAAAATCTAAGAAAGTCAAAGCTGCTGGGCAAGAAAGCAAGCCAATTGTTCAAATGAGCCCTCTCTTGGAAAACCAATCCTCAAAGGCTAATTTAAAGAATGAAGTTATTCCTGGGACATCAAACAGTTCCCGTCTATCTGAATTTCATGAGGCAAAGGCTGCTAAGAGTTCCACTTTTCTACCAACGACATGCTCTTCTGAAATGCCTTTATCATCTGCTAATGTTACCACTAATATACCTGTTATCCCGGGAGGGTATCTGCAGACATTGTTAGATGCTTCTGACTTGTCAAATAACACTAGTATCTCATACTTCAGCCACCATTCTCCAGAGCAAAATGAAGGCAGCCTCACTCAAACCGAAAAATCATTTGTACCCCTCCAGCCTACCCAGGACTGTGTGCTCACCTCATCCTCTGACTCTGAGCTGCAGCAGTCATCTCATAACTTCAAAATGGAATCAAGCAACTATAGAAATGTGTGGCCCAACAAGGCTACTTCTGGCACCCAGGAATTCATGGCTGAAGTCTCAAGGGAGATAGCCCCAACCCAATCCAGTGAATTTGGAGCCTCCCAAGTAGTCTCCATGGAAAATAACCTCACACCTACAACATACAATCCAATCTGTCTCAATAGTGGTGGCAGTAATTGCAACAAGGTCCTGTATGACTCCATGCAAGATACCCAACTCCCATCTGATGACTCTTACCAATTATGTCACTTTAATAATGGAGAGATCTGCTTTCCTTTCCAGCAGGGGCCAGTCAATATGGATGATGGTCGGCTCTTTAGCTTTGATTCAATGGCCCCACTCTCTGTCAGCTCAAGTAATTATTGCTCCTTAAGCTTGAAGTCCTGTGAAAAGGATGGCGATGATGATATCACTGATGACTTCCTGGCCCATTGCAGCCCTAAGCTGGTGATCCAGCAAAGCATTGATGAGATAGCACCACTAAAGGAGTCCACTGACCTCCTGGATATATCCAACTTCACCCCTGACAAATTCCGCCACTCTTCCCTTTCAGAGATGTCCCCACCGGACACCCCTAGTCTTTCCCCTCAAATTACCAGATGTGAGAGTATGAAGACACTAGGAACACTAAAGGGGTTCCAAGAGGGTGTCCCAGGACCATTGGACAGTGTGGAAAAAATCAAGTGGGACTGCAGTACCCTTTCACGGCAGGTCCAAATGGAGGATGGATTTACTTTAAATAATCACCAGTTTCAGTTCCATATGTTCAATGATGAGGATTCTGTCAGCCTGCTCCAAAAAAACCCTTGCCTGTCAACATTTAATGATCCATCTGGTCAAATTAGTACCAACAACAAAGTGTCAAAATCAAGAAAGAAAAGTTCACCCAGCAAGAGTGGGGCTATGAACCAAAGCTCTTCTCAGAAAAACACCAGGAAAAAATCCCTCAAAGGTAACAACAAGGGGATTGAAAAACCACCTGGCAAAAACTCCCGCCAGGTCCCTAAGTCCACAAAGAAAGGGAAATACATGGCTGCCATCAATGGAGAGAAAATGCAAATTGGCATTGGCCGTGGGGGAAGCCAAACCAACACCATATCCTCCACTGGGAAGACATTGGCTGAATGTATCCAACATGGTGGCCCTATGGCCTCTATGAAGATGCCAAGTCAAAAGGGACTTTCTGGAGATTGGGCCTTGGGGAAGGAGAGCAGCCCAGGCTGGAGTGATATGAGCATGGGCACCAACACCAACAGCCTTCTGGATGATGACCAACGGGAATTTCAGGAGCCTTCCTATATCTTGTCCAACATTGCCTCTGGTATGGCAGATGTGCAGAGATTCATGATGGCCTCCATAGAGCCCCTTTGGGAACCCATGGAGCACCATGGAGATCCCAACATATTCTACTCCCCTGAGTCCAATAGTCTAAAATTAAAAACCCTCAAAATATTGGCTGGGACACCACAGGAGTCTAAGAAAAAGATCAACAGTGGGTCCCAAGGAGCCACTAAGAATCACAGGTCAATCAAGGGTGTGAGCAAAAGCAATGGGAAAACAGCAATAGGTGATCCTGGTCGTGCAAACATGCCTGGTTATAACGAGGACTCTCGCTCTACCTTCTTTGATAAAAAGTATAGTAACATGAGCACTTTAGGCAATAACGGACCGACACACAAAAAGTTGTATCGTCACAAATCCAGCTCCAAGGCCCTGAGAGATGAGAAATGTAAGGGAAAGCACATGGAGCGAGAACAGGTCCACAAGGATGAGTCTGGGACAGCTTCTTTTGAAAAACTGAGGGATTCCGACTACAATCTCCTAAAAGCAGAAACAACCTTTTGGGTTTTACCTGTGTTTGAAGAAGAGACTCGCATTTTCCAGAAAGACATTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRK
VGSMDNQQDKAIVASANGENTLINGVKENDSEDQDVAMKSFAALEAAAPI
QPTPVAQKETLMYPRGLLPLPSKKPCMQSPPSPLGLIEAPEHAANSASVN
AISLTSGIAKGLNTWSLPNECEKAPFAIMEPAGMSALNGDCLMQPSRTCL
GCFMESKDAVDPEPGISLKVGDLNRDYETCAVSDIGIQCINAGENMKYGE
QLLSDQLLGFPLHKSRAGDRRETEKPDIDLEDPAQKSYYEALLLDKCNTE
EALLANSNQDWGYFETFISESKIELLDLCSKNEESENLFSEEDVDNYMFD
DDESTLGSDVCSLKIRYESFQDNVRDKTTLLMQEDAQFNFFPSVFTTCPK
RESKSGALKQSSDFSQFKVPDVSIIWGEEDKNLDKKKGKEEGQEDKGVEK
KDGKDNGEKPALNKPCSGTEVEQLKNPKQGHLANSLETSGSFSDDSSFIE
ISYDAMGEIKDCSRYMARDTNSGSSSSQQNYGLRAKRKVRYSEDYLYDVD
SLEGEKVNERKEWLPVGSKEEDDDEWCPKKRRKVTRKEPPVIIKYIIINR
FKGEKNMLVKLGKVDASETTVNLSENQLNKYAKLAPLKGFWQKKKKQRNT
NTDSIKTPFSQKQSFEPGSFEVSFLPPARKRKSKLGNRHRIQRIPSIEIS
ASSKQISLCNDQRHASNHKEDGGLKGTLKSAPLGAPSCANGSHLNDITGP
DSVKVKAQDTEFKGPERKVLNKIKFKSEARLKSKKVKAAGQESKPIVQMS
PLLENQSSKANLKNEVIPGTSNSSRLSEFHEAKAAKSSTFLPTTCSSEMP
LSSANVTTNIPVIPGGYLQTLLDASDLSNNTSISYFSHHSPEQNEGSLTQ
TEKSFVPLQPTQDCVLTSSSDSELQQSSHNFKMESSNYRNVWPNKATSGT
QEFMAEVSREIAPTQSSEFGASQVVSMENNLTPTTYNPICLNSGGSNCNK
VLYDSMQDTQLPSDDSYQLCHFNNGEICFPFQQGPVNMDDGRLFSFDSMA
PLSVSSSNYCSLSLKSCEKDGDDDITDDFLAHCSPKLVIQQSIDEIAPLK
ESTDLLDISNFTPDKFRHSSLSEMSPPDTPSLSPQITRCESMKTLGTLKG
FQEGVPGPLDSVEKIKWDCSTLSRQVQMEDGFTLNNHQFQFHMFNDEDSV
SLLQKNPCLSTFNDPSGQISTNNKVSKSRKKSSPSKSGAMNQSSSQKNTR
KKSLKGNNKGIEKPPGKNSRQVPKSTKKGKYMAAINGEKMQIGIGRGGSQ
TNTISSTGKTLAECIQHGGPMASMKMPSQKGLSGDWALGKESSPGWSDMS
MGTNTNSLLDDDQREFQEPSYILSNIASGMADVQRFMMASIEPLWEPMEH
HGDPNIFYSPESNSLKLKTLKILAGTPQESKKKINSGSQGATKNHRSIKG
VSKSNGKTAIGDPGRANMPGYNEDSRSTFFDKKYSNMSTLGNNGPTHKKL
YRHKSSSKALRDEKCKGKHMEREQVHKDESGTASFEKLRDSDYNLLKAET
TFWVLPVFEEETRIFQKDI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45669
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee