Synonyms
ATG1, ATG1A, hATG1, UNC51, Unc51.1
Plasmid
pBabe.hygro.FLAG.DU ULK1 K46I sprot
Parent Plasmid
pBabeD hygro FLAG
Expressed
FLAG-ULK1 K46I sprot
Genbank
NM_003565.2 GI:225637564 c2141t (P714L (exisiting SNP)
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
GGATCCCTGGAGCCCGGCCGCGGCGGCACAGAGACCGTGGGCAAGTTCGAGTTCTCCCGCAAGGACCTGATCGGCCACGGCGCCTTCGCGGTGGTCTTCAAGGGCCGCCACCGCGAGAAGCACGATTTGGAGGTCGCCGTCATCTGCATTAACAAGAAGAACCTCGCCAAGTCTCAGACGCTGCTGGGGAAGGAAATCAAAATCCTGAAGGAACTGAAACATGAAAACATCGTGGCCCTGTACGACTTCCAGGAAATGGCTAATTCTGTCTACCTGGTTATGGAGTACTGCAACGGTGGGGACCTGGCCGACTACCTGCACGCCATGCGCACGCTGAGCGAGGACACCATCAGGCTCTTCCTGCAGCAGATCGCGGGCGCCATGCGGCTTCTGCACAGCAAAGGCATCATCCACCGCGACCTGAAACCGCAGAACATCCTGCTGTCCAACCCCGCCGGCCGCCGCGCCAACCCCAACAGCATCCGCGTCAAGATCGCTGACTTCGGCTTCGCTAGATATTTGCAATCGAACATGATGGCGGCCACACTCTGCGGCTCCCCCATGTACATGGCCCCCGAGGTCATCATGTCCCAGCACTACGACGGGAAGGCGGACCTGTGGAGCATCGGCACCATCGTCTACCAGTGCCTGACGGGGAAGGCGCCCTTCCAGGCCAGCAGCCCCCAGGACCTGCGCCTGTTCTACGAGAAGAACAAGACGTTGGTCCCCACCATCCCCCGGGAGACCTCGGCCCCGCTGCGGCAGCTGCTCCTGGCCCTACTGCAACGCAACCACAAGGACCGCATGGACTTCGATGAGTTTTTTCATCACCCTTTCCTCGATGCCAGCCCCTCGGTCAGGAAATCCCCACCCGTGCCTGTGCCCTCGTACCCAAGCTCGGGGTCCGGCAGCAGCTCCAGCAGCAGCTCCACCTCCCACCTGGCCTCCCCGCCGTCCCTGGGCGAGATGCAGCAGCTGCAGAAGACCCTGGCCTCCCCGGCTGACACCGCTGGCTTCCTGCACAGCTCCCGGGACTCTGGTGGCAGCAAGGACTCTTCCTGTGACACAGATGACTTCGTCATGGTCCCCGCGCAGTTTCCAGGTGACCTGGTGGCTGAGGCGCCCAGTGCCAAACCCCCGCCAGACAGCCTGATGTGCAGTGGGAGCTCACTGGTGGCCTCTGCGGGCTTGGAGAGCCACGGCCGGACCCCATCTCCATCCCCACCCTGCAGCAGCTCCCCCAGTCCCTCAGGCCGGGCTGGCCCGTTCTCCAGCAGCAGGTGCGGCGCCTCTGTCCCCATCCCAGTCCCCACGCAGGTGCAGAACTACCAGCGCATTGAGCGAAACCTGCAGTCACCCACCCAGTTCCAAACACCTCGGTCCTCTGCCATCCGCAGGTCAGGCAGCACCAGCCCCCTGGGCTTTGCAAGGGCCAGCCCCTCGCCCCCTGCCCACGCTGAGCATGGAGGCGTCCTGGCCAGGAAGATGTCTCTGGGTGGAGGCCGGCCCTACACGCCATCTCCTCAAGTTGGAACCATCCCTGAGCGGCCAGGCTGGAGCGGGACGCCCTCCCCACAGGGAGCTGAGATGCGGGGTGGCAGGTCCCCTCGTCCAGGCTCCTCTGCACCCGAGCACTCTCCCCGCACTTCCGGGCTGGGCTGCCGCCTGCACAGCGCCCCCAACCTGTCTGACTTGCACGTCGTCCGCCCCAAGCTGCCCAAACCCCCCACGGACCCCCTGGGAGCTGTGTTCAGCCCACCACAGGCCAGCCCTCCCCAGCCGTCCCACGGCCTGCAGTCCTGCCGGAACCTGCGGGGCTCACCCAAGCTGCCCGACTTCCTGCAGCGAAACCCCCTGCCCCCCATCCTGGGCTCCCCCACCAAGGCTGTGCCCTCCTTTGACTTCCCGAAGACCCCCAGCTCCCAGAACCTGCTGGCCCTCCTAGCCCGGCAGGGCGTGGTGATGACGCCCCCTCGAAACCGGACGCTGCCCGACCTCTCGGAGGTGGGACCCTTCCATGGTCAGCCGTTGGGCCCTGGCCTGCGGCCAGGCGAGGACCCCAAGGGCCCCTTTGGCCGGTCTTTCAGCACCAGCCGCCTCACTGACCTGCTCCTTAAGGCGGCGTTTGGGACACAAGCCCCGGACCTGGGCAGCACGGAGAGCCTGCAGGAGAAGCCCATGGAGATCGCACCCTCAGCTGGCTTTGGAGGGAGCCTGCACCCAGGAGCCCGTGCTGGGGGCACCAGCAGCCCTTCCCCGGTGGTCTTCACCGTGGGCTCTCCCCCGAGCGGGAGCACGCCCCCCCAGGGCCCCCGCACCAGGATGTTCTCAGCGGGCCCCACTGGCTCTGCCAGCTCTTCTGCCCGCCACCTGGTGCCTGGGCCCTGCAGCGAGGCCCCAGCCCCTGAGCTCCCTGCTCCAGGACACGGCTGCAGCTTTGCCGACCCCATTGCTGCGAACCTGGAGGGGGCTGTGACCTTCGAGGCCCCCGACCTCCCTGAGGAGACCCTCATGGAGCAAGAGCACACGGAGATCCTGCGTGGCCTGCGCTTCACGCTGCTGTTCGTGCAGCACGTCCTGGAGATCGCAGCCCTGAAGGGCAGCGCCAGTGAGGCGGCGGGGGGCCCTGAGTACCAGCTGCAGGAGAGTGTGGTGGCCGACCAGATCAGCCTGCTGAGCCGAGAATGGGGCTTCGCGGAACAGCTGGTGCTGTACCTGAAGGTGGCCGAGCTACTGTCCTCCGGCCTGCAAAGTGCCATCGACCAGATCCGGGCCGGCAAGCTCTGCCTGTCGTCCACTGTGAAGCAGGTGGTGCGCAGGCTGAATGAGCTGTACAAGGCCAGCGTGGTGTCCTGCCAGGGCCTGAGCCTGCGGCTGCAGCGCTTCTTCCTGGACAAGCAGCGGCTCCTGGACCGCATTCACAGCATCACTGCCGAGAGGCTCATCTTCAGCCACGCTGTGCAGATGGTGCAGTCGGCTGCCCTGGACGAGATGTTCCAGCACCGTGAGGGCTGCGTCCCACGCTACCACAAGGCCCTGCTGCTCCTGGAGGGGCTGCAGCACATGCTCTCGGACCAGGCCGACATCGAGAACGTCACCAAGTGCAAGCTGTGCATTGAGCGGAGACTCTCGGCGCTGCTGACTGGCATCTGTGCCTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MDYKDDDDKGSLEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKH
DLEVAVICINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVY
LVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDL
KPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMA
PEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTL
VPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPP
VPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLH
SSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSL
VASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQ
NYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGV
LARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSS
APEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASP
PQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSS
QNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPF
GRSFSTSRLTDLLLKAAFGTQAPDLGSTESLQEKPMEIAPSAGFGGSLHP
GARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHL
VPGPCSEAPAPELPAPGHGCSFADPIAANLEGAVTFEAPDLPEETLMEQE
HTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISL
LSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRL
NELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQ
SAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIER
RLSALLTGICA*
DLEVAVICINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVY
LVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDL
KPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMA
PEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTL
VPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPP
VPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLH
SSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSL
VASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQ
NYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGV
LARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSS
APEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASP
PQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSS
QNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPF
GRSFSTSRLTDLLLKAAFGTQAPDLGSTESLQEKPMEIAPSAGFGGSLHP
GARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHL
VPGPCSEAPAPELPAPGHGCSFADPIAANLEGAVTFEAPDLPEETLMEQE
HTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISL
LSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRL
NELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQ
SAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIER
RLSALLTGICA*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU45697
Unit Source
Location
PPU
MTA
Notes
N.B. Grow at 30 degrees.
Price
£125.00
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