cDNA Clone Parkin

• Plasmid: pGEX6P-1 parkin 1-76 TAG amber
• Parent Plasmid: pGEX6P1
• Expressed: GST-Parkin [residues 1-76] [mutate Ser65 codon to TAG]
• Genbank: O60260
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGATAGTGTTTGTCAGGTTCAACTCCAGCCATGGTTTCCCAGT
GGAGGTCGATTCTGACACCAGCATCTTCCAGCTCAAGGAGGTGGTTGCTA
AGCGACAGGGGGTTCCGGCTGACCAGTTGCGTGTGATTTTCGCAGGGAAG
GAGCTGAGGAATGACTGGACTGTGCAGAATTGTGACCTGGATCAGCAGAG
CATTGTTCACATTGTGCAGAGACCGTGGAGAAAATagGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCDLDQQSIVHIVQRPWRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43028
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dear Rachel - as discussed, please use clone DU37369 and mutate Ser65 codon to an Amber TAG stop codon. Urgent please.