cDNA Clone HPK1 (mouse)

• Synonyms: MAP4K1, Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1
• Plasmid: pCMV FLAG HPK1 (mouse) S452A
• Parent Plasmid: pCMV5 FLAG2
• Expressed: FLAG HPK1 S452A
• Genbank: DU43971
• Tag: FLAG
• Species: Mouse

Sequence of Insert

GCGGCCGCGATGGCCCTTGTGGACCCCGACATTTTTAATAAGGACCCTCG
AGAACACTATGATCTACTGCAACGACTGGGTGGTGGAACTTATGGAGAAG
TATTCAAGGCTCGCGACAAGGTGTCTAAGGACCTGGTGGCTTTGAAGATG
GTGAAGATGGAGCCTGATGATGACGTCGCCACCCTTCAGAAGGAGATTCT
CATGCTGAAAACTTGCCGGCACGCCAACATCGTGGCTTACCATGGCAGTT
ACCTCTGGCTGCAGAAGCTGTGGATCTGCATGGAATTCTGTGGAGCTGGT
TCTCTCCAGGACATCTACCAAGTGACTGGCTCTCTGTCCGAGCTCCAGAT
CAGCTATGTGTGCCGGGAAGTGCTTCAGGGACTGGCCTACCTGCATTCAG
AGAAGAAGATACATCGAGACATCAAGGGAGCCAACATCCTCATCAACGAC
TGCGGGGAGGTTAAGCTGGCTGACTTTGGGATCTCAGCCCAGATTGGGGC
GACACTGGCCCGGCGCCTTTCTTTCATTGGGACACCGTACTGGATGGCTC
CAGAGGTGGCAGCGGTGGCCCTGAAGGGTGGATACAATGAGCTGTGCGAC
ATCTGGTCTCTTGGCATCACAGCCATCGAGCTGGCTGAGCTTCAGCCCCC
TCTCTTTGATGTGCACCCTCTCAGGGTTCTCTTCCTCATGACCAAGAGCG
GGTACCAGCCTCCTCGGCTGAAGGAAAAGAGCAGATGGTCATCTTCCTTC
CACAACTTTGTCAAAGTCACCCTCACTAAGAACTCCAAGAAACGCCCCAG
TGCCACCAAGATGCTCAGTCATCAGCTGGTGTCCCAGCCAGGGCTGAACA
GAGGCCTGATCTTGGATCTTCTTGACAAAATGAAGAATCCTGGGAAGGGG
CTGCCTGTTGATATTGAAGATGAGGAGCCTGAGCCACCCCCTGCCATCCC
TCGGCGGATCAGATCTACCTACCGGGCCAGCTCACTAGGGATCCCAGATG
CAGACTGCTGCCGGAGGCAAATGGAGTTCCAGAGGCCCAGATGCGTGGAC
TGCAGACCTCAGGCTGAAACTGTTCGCTTATATCCCCCTGCACACTTCGG
GAGCACCAGTCCCAGGAGTCAGCTGTCAGATTCTGATGACGACTATGATG
ATGTGGACATCCCTGCCCCTTCAGAGAACATACCTCCTCCACTGCCCCCC
AAGCCCAAGTTTCGGTCTCCATCAGATGATGGTTCTGGAGGAATTAGGGA
CGATGGGCAATTGAGCCCAGGGGTTCTGGTTCGATGTGCAAGTGGGCCCC
CTCCACGTACTCCCCGACCTGGACCTCCCCCAGCGACCTGCAGCCCTCAC
CTCACAGCTCGCgCAGATCCCTCATTGTGGAACCCAGCTGCTCCGGAGCC
AGGCCAGCCCCCACTCGTACCCCCAAGGAAGGAAAAGATGAGGGGAAAGA
TGGAGAACGAGAAGCGCAGAGAGAAATATCCCCTTCTTGTGAAATTATTC
AATGGCTGTCCCCTTCAGATCCACAGCACAGCTGCCTGGACACATCCCTC
TACAAAAGATCAGAACCTGCTCCTGGGGGCAGAGGAAGGCATTTTCATAC
TGAACCGGAATGATCAGGAGGCCACGCTGGAAATGATCTTCCCTGGCCGC
ACAACCTGGCTCTATTGCATCAACAACCTCCTCATGTCTCTCTCAGGGAA
GACACCCTACCTGTATTCTCATAGCATCCTGGGCCTGCTTGAAAGGAAAG
ACGGCAGAACGGGAAGCCCCATTGCTCACATTAGCCCTCACCGGTTACTG
GCAAGAAAGAACATGGTCTCCAGTAAGATCCAGGACACCAAAGGCTGCCG
GGCTTGCTGTGTGGCTGAGAGTGCTAGCTCTGGGGGCCCATTCTTATGTG
GGGCATTGGAGACGTCTGTGGTTTTGCTCCAGTGGTACCAACCAATGAAC
AAATTCTTGCTTGTCCGGCAGGTGCTGTTCCCGCTGCCCACGCCGCTGCC
GGTGTTCACGCTGCTGACAACGCCGGGCTCCGAGCTGCCAGCTGTGTGCA
TCGGCGTGAGCCCGGGGCAGGCGGCAAAGTCGGTGCTCTTCCACACCGTG
CGCTTCGGTGCGCTGTCCTGCTGGCTGGACGACTCCAGCACGGAGCACAA
GGGACCAGTGCAGGTGATCCAAGTAAAGGAAGACATGGTGATGGTGTTGA
TGGATGGCTCTCTAAAGCTAGTGACCCCAGAGGGGGCACCAGCTCCTGGG
CTCCGCACCCCTGAGATCCCCATGACAGAAGCGGTCGAGGCTGTGGCTAT
GGTTGAAGATCGACTCGAGGCCTTCTGGAAGCATGGAGTTCAGGTGTGGG
CTCCAGGCTTGAAACAGCCGCTGCAGGAGCTGAGAGACCCCACCCTCACC
TTCCGTCTGCTCTGCTCCCCCAGGCCGGTAGTGGTGGAGACAAGGCCCAC
GGATGACCCTACGGCCCCCAGCAACCTCTACATCCAGGAATGAGCGGCCG
C

Amino Acid Sequence

MDYKDDDDKGIPGIPGSTRAAAMALVDPDIFNKDPREHYDLLQRLGGGTY
GEVFKARDKVSKDLVALKMVKMEPDDDVATLQKEILMLKTCRHANIVAYH
GSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYL
HSEKKIHRDIKGANILINDCGEVKLADFGISAQIGATLARRLSFIGTPYW
MAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMT
KSGYQPPRLKEKSRWSSSFHNFVKVTLTKNSKKRPSATKMLSHQLVSQPG
LNRGLILDLLDKMKNPGKGLPVDIEDEEPEPPPAIPRRIRSTYRASSLGI
PDADCCRRQMEFQRPRCVDCRPQAETVRLYPPAHFGSTSPRSQLSDSDDD
YDDVDIPAPSENIPPPLPPKPKFRSPSDDGSGGIRDDGQLSPGVLVRCAS
GPPPRTPRPGPPPATCSPHLTARADPSLWNPAAPEPGQPPLVPPRKEKMR
GKMENEKRREKYPLLVKLFNGCPLQIHSTAAWTHPSTKDQNLLLGAEEGI
FILNRNDQEATLEMIFPGRTTWLYCINNLLMSLSGKTPYLYSHSILGLLE
RKDGRTGSPIAHISPHRLLARKNMVSSKIQDTKGCRACCVAESASSGGPF
LCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLPVFTLLTTPGSELPA
VCIGVSPGQAAKSVLFHTVRFGALSCWLDDSSTEHKGPVQVIQVKEDMVM
VLMDGSLKLVTPEGAPAPGLRTPEIPMTEAVEAVAMVEDRLEAFWKHGVQ
VWAPGLKQPLQELRDPTLTFRLLCSPRPVVVETRPTDDPTAPSNLYIQE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46076
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee