cDNA Clone TRPM6

• Synonyms: CHAK2, HMGX, HOMG, HOMG1, HSH
• Plasmid: pGEX TRPM6 200-800
• Parent Plasmid: pGEX6P1
• Expressed: GST-TRPM6 200-800
• Genbank: NM_017662.4
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCATGAGAAAAATCTGGACAGTTGGAATCCCTCCTTGGGGTGTCATTGAGAACCAGAGAGACCTTATTGGAAAAGATGTGGTGTGCCTGTACCAGACTCTGGATAACCCCCTCAGCAAGCTCACAACACTCAACAGCATGCACTCGCACTTCATCCTGTCTGATGATGGGACCGTGGGCAAGTATGGAAATGAAATGAAGCTCAGAAGGAACCTGGAGAAGTACCTCTCTCTGCAGAAAATACACTGCCGCTCAAGACAAGGCGTGCCGGTCGTGGGGCTGGTGGTGGAAGGCGGTCCCAACGTCATCCTGTCAGTGTGGGAGACTGTCAAGGACAAGGACCCAGTGGTGGTGTGTGAGGGTACAGGTAGGGCGGCTGACCTCCTGGCCTTCACACACAAACACCTGGCAGATGAAGGGATGCTGCGACCTCAGGTGAAAGAGGAGATCATCTGCATGATTCAGAACACTTTCAACTTTAGTCTTAAACAGTCCAAGCACCTTTTCCAAATTCTAATGGAGTGTATGGTTCACAGGGATTGTATTACCATATTTGATGCTGACTCTGAAGAGCAGCAAGACCTGGACTTAGCAATCCTAACAGCTTTGCTGAAGGGCACAAATTTATCAGCGTCAGAGCAATTAAATCTGGCAATGGCTTGGGACAGGGTGGACATTGCCAAGAAACATATCCTAATTTATGAACAACACTGGAAGCCTGATGCCCTGGAACAAGCAATGTCAGATGCTTTAGTGATGGATCGGGTGGATTTTGTGAAGCTCTTAATAGAATATGGAGTGAACCTCCATCGCTTTCTTACCATCCCTCGACTGGAAGAGCTCTACAATACAAAACAAGGACCTACTAATACACTCTTGCATCATCTCGTCCAAGATGTGAAACAGCATACCCTTCTTTCAGGCTACCGAATAACCTTGATTGACATTGGATTAGTAGTAGAATACCTCATTGGTAGAGCATATCGCAGCAACTACACTAGAAAACATTTCAGAGCCCTCTACAACAACCTCTACAGAAAATACAAGCACCAGAGACACTCCTCAGGAAATAGAAATGAGTCTGCAGAAAGTACGCTGCACTCCCAGTTCATTAGAACTGCACAGCCATACAAATTCAAGGAAAAGTCTATAGTCCTTCATAAATCAAGGAAGAAGTCAAAAGAACAAAATGTATCAGATGACCCTGAGTCTACTGGCTTTCTTTACCCTTACAATGACCTGCTGGTTTGGGCTGTGCTGATGAAAAGGCAGAAGATGGCTATGTTCTTCTGGCAGCATGGAGAGGAGGCCACGGTTAAAGCCGTGATTGCGTGTATCCTCTACCGGGCAATGGCCCATGAAGCTAAGGAGAGTCACATGGTGGATGATGCCTCAGAAGAGTTGAAGAATTACTCAAAACAGTTTGGCCAGCTGGCTCTGGACTTGTTGGAGAAGGCATTCAAGCAGAATGAGCGCATGGCCATGACGCTGTTGACGTATGAACTCAGGAACTGGAGCAATTCGACCTGCCTTAAACTGGCCGTGTCGGGAGGATTACGACCCTTTGTTTCACATACTTGTACCCAGATGCTACTGACAGACATGTGGATGGGGAGGCTGAAAATGAGGAAAAACTCTTGGTTAAAGATTATTATAAGCATTATTTTACCACCCACCATTTTGACACTGGAATTTAAAAGCAAAGCTGAGATGTCACATGTTCCCCAGTCCCAGGACTTCCAATTTATGTGGTATTACAGTGACCAGAACGCCAGCAGTTCCAAAGAAAGTGCTTCTGTGAAAGAGTATTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAMRKIWTVGIPPWGVIENQ
RDLIGKDVVCLYQTLDNPLSKLTTLNSMHSHFILSDDGTVGKYGNEMKLR
RNLEKYLSLQKIHCRSRQGVPVVGLVVEGGPNVILSVWETVKDKDPVVVC
EGTGRAADLLAFTHKHLADEGMLRPQVKEEIICMIQNTFNFSLKQSKHLF
QILMECMVHRDCITIFDADSEEQQDLDLAILTALLKGTNLSASEQLNLAM
AWDRVDIAKKHILIYEQHWKPDALEQAMSDALVMDRVDFVKLLIEYGVNL
HRFLTIPRLEELYNTKQGPTNTLLHHLVQDVKQHTLLSGYRITLIDIGLV
VEYLIGRAYRSNYTRKHFRALYNNLYRKYKHQRHSSGNRNESAESTLHSQ
FIRTAQPYKFKEKSIVLHKSRKKSKEQNVSDDPESTGFLYPYNDLLVWAV
LMKRQKMAMFFWQHGEEATVKAVIACILYRAMAHEAKESHMVDDASEELK
NYSKQFGQLALDLLEKAFKQNERMAMTLLTYELRNWSNSTCLKLAVSGGL
RPFVSHTCTQMLLTDMWMGRLKMRKNSWLKIIISIILPPTILTLEFKSKA
EMSHVPQSQDFQFMWYYSDQNASSSKESASVKEY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44744
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 200-800; ; All TRPM6 plasmids MUST be grown at 30 degrees C or less to prevent recombination.