cDNA Clone SYT1

• Synonyms: P65, SVP65, SYT
• Plasmid: pGEX TEVb SYT1 81-end
• Parent Plasmid: pGEX6P1
• Expressed: GST-TEVb-SYT1 81-end
• Genbank: NM_001135805.1
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAAACCTGTATTTTCAGAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGAAAGGAAAAAGGAGGGAAGAATGCCATTAACATGAAAGATGTAAAAGACTTAGGGAAGACGATGAAAGATCAGGCCCTCAAGGATGATGATGCTGAAACTGGATTGACAGATGGAGAAGAAAAAGAAGAACCCAAAGAAGAGGAGAAACTGGGAAAACTTCAGTATTCACTGGATTATGATTTCCAAAATAACCAGCTGCTGGTAGGGATCATTCAGGCTGCCGAACTGCCCGCCTTGGACATGGGGGGCACATCTGATCCTTACGTGAAAGTGTTTCTGCTACCTGATAAGAAGAAGAAATTTGAGACAAAAGTCCACCGAAAAACCCTTAATCCTGTCTTCAATGAGCAATTTACTTTCAAGGTACCATACTCGGAATTGGGTGGCAAAACCCTAGTGATGGCTGTATATGATTTTGATCGTTTCTCTAAGCATGACATCATTGGAGAATTTAAAGTCCCTATGAACACAGTGGATTTTGGCCATGTAACTGAGGAATGGCGTGACCTGCAAAGTGCTGAGAAGGAAGAGCAAGAGAAATTGGGTGATATCTGCTTCTCCCTTCGCTACGTACCTACTGCTGGTAAGCTGACTGTTGTCATTCTGGAGGCAAAGAACCTGAAGAAGATGGATGTGGGTGGCTTATCCGATCCTTATGTGAAGATTCATCTGATGCAGAATGGTAAGAGGCTGAAGAAGAAAAAGACAACAATTAAAAAGAACACACTTAACCCCTACTACAATGAGTCATTCAGCTTTGAAGTACCTTTTGAACAAATCCAGAAAGTGCAGGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGATGCCATCGGCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAACCCCAGGCGACCTATTGCCCAGTGGCACACCCTGCAGGTAGAGGAGGAAGTTGATGCCATGCTGGCCGTCAAGAAGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMENLYFQKKCLFKKKNKKK
GKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEK
LGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKK
KFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIG
EFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTV
VILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNE
SFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWS
DMLANPRRPIAQWHTLQVEEEVDAMLAVKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44756
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 81-end. Blunt cleavage using TEV.